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遺伝子ノックアウト細胞株 検証済リコンビナントRabMAb

Anti-Apolipoprotein E 抗体 [EPR19378] (ab183596)

Reviews (1) Submit a question References (8)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein E antibody [EPR19378] (ab183596)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein E antibody [EPR19378] (ab183596)
  • Western blot - Anti-Apolipoprotein E antibody [EPR19378] (ab183596)
  • Western blot - Anti-Apolipoprotein E antibody [EPR19378] (ab183596)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein E antibody [EPR19378] (ab183596)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein E antibody [EPR19378] (ab183596)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein E antibody [EPR19378] (ab183596)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein E antibody [EPR19378] (ab183596)
  • Immunohistochemistry (Frozen sections) - Anti-Apolipoprotein E antibody [EPR19378] (ab183596)
  • Immunoprecipitation - Anti-Apolipoprotein E antibody [EPR19378] (ab183596)
  • Anti-Apolipoprotein E antibody [EPR19378] (ab183596)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19378] to Apolipoprotein E
  • Suitable for: IHC-P, WB, IP, IHC-Fr
  • Knockout validated
  • Reacts with: Mouse, Rat

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-Apolipoprotein E antibody [EPR19378]
    Apolipoprotein E 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR19378] to Apolipoprotein E

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: IHC-P, WB, IP, IHC-Frmore details

  • 種交差性

    交差種: Mouse, Rat

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Mouse plasma; Mouse liver, brain and kidney lysates; Rat plasma; Rat liver, brain and kidney lysates. IHC-P: Mouse cerebral cortex and liver tissues; Rat cerebral cortex and spleen tissues. IHC-Fr: Mouse cortex tissue. IP: Mouse plasma.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab183596の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P
1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
1/5000. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
IP (1)
1/30.
IHC-Fr
1/500.
特記事項
IHC-P
1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
1/5000. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
IP
1/30.
IHC-Fr
1/500.

ターゲット情報

  • 機能

    Mediates the binding, internalization, and catabolism of lipoprotein particles. It can serve as a ligand for the LDL (apo B/E) receptor and for the specific apo-E receptor (chylomicron remnant) of hepatic tissues.

  • 組織特異性

    Occurs in all lipoprotein fractions in plasma. It constitutes 10-20% of very low density lipoproteins (VLDL) and 1-2% of high density lipoproteins (HDL). APOE is produced in most organs. Significant quantities are produced in liver, brain, spleen, lung, adrenal, ovary, kidney and muscle.

  • 関連疾患

    Defects in APOE are a cause of hyperlipoproteinemia type 3 (HLPP3) [MIM:107741]; also known as familial dysbetalipoproteinemia. Individuals with HLPP3 are clinically characterized by xanthomas, yellowish lipid deposits in the palmar crease, or less specific on tendons and on elbows. The disorder rarely manifests before the third decade in men. In women, it is usually expressed only after the menopause. The vast majority of the patients are homozygous for APOE*2 alleles. More severe cases of HLPP3 have also been observed in individuals heterozygous for rare APOE variants. The influence of APOE on lipid levels is often suggested to have major implications for the risk of coronary artery disease (CAD). Individuals carrying the common APOE*4 variant are at higher risk of CAD.
    Genetic variations in APOE are associated with Alzheimer disease type 2 (AD2) [MIM:104310]. It is a late-onset neurodegenerative disorder characterized by progressive dementia, loss of cognitive abilities, and deposition of fibrillar amyloid proteins as intraneuronal neurofibrillary tangles, extracellular amyloid plaques and vascular amyloid deposits. The major constituent of these plaques is the neurotoxic amyloid-beta-APP 40-42 peptide (s), derived proteolytically from the transmembrane precursor protein APP by sequential secretase processing. The cytotoxic C-terminal fragments (CTFs) and the caspase-cleaved products such as C31 derived from APP, are also implicated in neuronal death. Note=The APOE*4 allele is genetically associated with the common late onset familial and sporadic forms of Alzheimer disease. Risk for AD increased from 20% to 90% and mean age at onset decreased from 84 to 68 years with increasing number of APOE*4 alleles in 42 families with late onset AD. Thus APOE*4 gene dose is a major risk factor for late onset AD and, in these families, homozygosity for APOE*4 was virtually sufficient to cause AD by age 80. The mechanism by which APOE*4 participates in pathogenesis is not known.
    Defects in APOE are a cause of sea-blue histiocyte disease (SBHD) [MIM:269600]; also known as sea-blue histiocytosis. This disorder is characterized by splenomegaly, mild thrombocytopenia and, in the bone marrow, numerous histiocytes containing cytoplasmic granules which stain bright blue with the usual hematologic stains. The syndrome is the consequence of an inherited metabolic defect analogous to Gaucher disease and other sphingolipidoses.
    Defects in APOE are a cause of lipoprotein glomerulopathy (LPG) [MIM:611771]. LPG is an uncommon kidney disease characterized by proteinuria, progressive kidney failure, and distinctive lipoprotein thrombi in glomerular capillaries. It mainly affects people of Japanese and Chinese origin. The disorder has rarely been described in Caucasians.

  • 配列類似性

    Belongs to the apolipoprotein A1/A4/E family.

  • 翻訳後修飾

    Synthesized with the sialic acid attached by O-glycosidic linkage and is subsequently desialylated in plasma. O-glycosylated with core 1 or possibly core 8 glycans. Thr-307 is a minor glycosylation site compared to Ser-308.
    Glycated in plasma VLDL of normal subjects, and of hyperglycemic diabetic patients at a higher level (2-3 fold).
    Phosphorylation sites are present in the extracelllular medium.

  • 細胞内局在

    Secreted.
  • Information by UniProt

  • 参照データベース

    • 別名

      • AD2 antibody
      • Apo-E antibody
      • APOE antibody
      • APOE_HUMAN antibody
      • APOEA antibody
      • Apolipoprotein E antibody
      • Apolipoprotein E3 antibody
      • ApolipoproteinE antibody
      • Apoprotein antibody
      • LDLCQ5 antibody
      • LPG antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein E antibody [EPR19378] (ab183596)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein E antibody [EPR19378] (ab183596)
      Immunohistochemical analysis of paraffin-embedded (A) Liver tissue from wild-type C57BL/6JGpt mice (B) Liver tissue from APOE knockout mice staining with ab183596 at 1/10000 dilution and ready-to-use Goat Anti-Rabbit IgG H&L (HRP) secondary. Counterstaining with hematoxylin. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins. The section was incubated with ab183596 for 30 mins at room temperature. Positive staining on Brown (A) Liver tissue from wild-type C57BL/6JGpt mice and no staining on (B) Liver tissue from APOE knockout mice. The immunostaining was performed on a Leica Biosystems BOND™ RX instrument
      The tissue samples were kindly provided by GemPharmatech. C57BL/6JGpt wildtype mice and APOE-KO homozygous mice (Strain ID: T001458).
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein E antibody [EPR19378] (ab183596)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein E antibody [EPR19378] (ab183596)
      Immunohistochemical analysis of paraffin-embedded (A) Brain tissue from wild-type C57BL/6JGpt mice and (B) Brian tissue from APOE knockout mice staining with ab183596 at 1/10000 dilution and ready-to-use Goat Anti-Rabbit IgG H&L (HRP) secondary. Counterstaining with hematoxylin. The section was incubated with ab183596 for 30 mins at room temperature.Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins. Positive staining on Brown (A) Brain tissue from wild-type C57BL/6JGpt mice and no staining on (B) Brian tissue from APOE knockout mice.The immunostaining was performed on a Leica Biosystems BOND™ RX instrument.
      The tissue samples were kindly provided by GemPharmatech. C57BL/6JGpt wildtype mice and APOE-KO homozygous mice (Strain ID: T001458)
    • Western blot - Anti-Apolipoprotein E antibody [EPR19378] (ab183596)
      Western blot - Anti-Apolipoprotein E antibody [EPR19378] (ab183596)
      All lanes : Anti-Apolipoprotein E antibody [EPR19378] (ab183596) at 1/1000 dilution

      Lane 1 : Wild-type mouse brain tissue lysate (male)
      Lane 2 : APOE knockout mouse brain tissue lysate (male)
      Lane 3 : Wild-type mouse liver tissue lysate (male)
      Lane 4 : APOE knockout mouse liver tissue lysate (male)
      Lane 5 : Wild-type mouse brain tissue lysate (female)
      Lane 6 : APOE knockout mouse brain tissue lysate (female)
      Lane 7 : Wild-type mouse liver tissue lysate (female)
      Lane 8 : APOE knockout mouse liver tissue lysate (female)

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 36 kDa
      Observed band size: 36 kDa


      Exposure time: 4 seconds


      The tissue samples were kindly provided by GemPharmatech. C57BL/6JGpt wildtype mice and APOE-KO homozygous mice (Strain ID: T001458).
    • Western blot - Anti-Apolipoprotein E antibody [EPR19378] (ab183596)
      Western blot - Anti-Apolipoprotein E antibody [EPR19378] (ab183596)
      All lanes : Anti-Apolipoprotein E antibody [EPR19378] (ab183596) at 1/5000 dilution

      Lane 1 : Mouse plasma
      Lane 2 : Mouse liver lysate
      Lane 3 : Rat plasma
      Lane 4 : Rat liver lysate
      Lane 5 : Mouse brain lysate
      Lane 6 : Rat brain lysate
      Lane 7 : Rat kidney lysate
      Lane 8 : Mouse kidney lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

      Predicted band size: 36 kDa
      Observed band size: 36 kDa



      Blocking/Dilution buffer: 5% NFDM/TBST.

      Exposure time: Lane 1,2,3 and 4: 4 seconds; Lane 5,6 and 7: 15 seconds; Lane 8: 1 minute.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein E antibody [EPR19378] (ab183596)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein E antibody [EPR19378] (ab183596)

      Immunohistochemical analysis of paraffin-embedded Mouse cerebral cortex tissue labeling Apolipoprotein E with ab183596 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on gliocytes of mouse cerebral cortex is observed. Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein E antibody [EPR19378] (ab183596)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein E antibody [EPR19378] (ab183596)

      Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Apolipoprotein E with ab183596 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on hepatocytes of mouse liver is observed. Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein E antibody [EPR19378] (ab183596)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein E antibody [EPR19378] (ab183596)

      Immunohistochemical analysis of paraffin-embedded Rat cerebral cortex tissue labeling Apolipoprotein E with ab183596 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on gliocytes of rat cerebral cortex is observed. Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein E antibody [EPR19378] (ab183596)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein E antibody [EPR19378] (ab183596)

      Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling Apolipoprotein E with ab183596 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on macrophages of rat spleen is observed. Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Frozen sections) - Anti-Apolipoprotein E antibody [EPR19378] (ab183596)
      Immunohistochemistry (Frozen sections) - Anti-Apolipoprotein E antibody [EPR19378] (ab183596)

      Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cortex tissue section labeling Apolipoprotein E with ab183596 at 1/500 dilution, followed by AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution. The result showed cytoplasmic staining on mouse cortex. The nuclear counter stain is DAPI (blue).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 at 1/1000 dilution.

    • Immunoprecipitation - Anti-Apolipoprotein E antibody [EPR19378] (ab183596)
      Immunoprecipitation - Anti-Apolipoprotein E antibody [EPR19378] (ab183596)

      Apolipoprotein E was immunoprecipitated from 1mg of Mouse plasma with ab183596 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab183596 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

      Lane 1: Mouse plasma, 10µg (Input).

      Lane 2: ab183596 IP in Mouse plasma.

      Lane 3: Rabbit IgG,monoclonal [EPR25A] - Isotype Control (ab172730) instead of ab183596 in Mouse plasma.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 1 second.

       

    • Anti-Apolipoprotein E antibody [EPR19378] (ab183596)
      Anti-Apolipoprotein E antibody [EPR19378] (ab183596)

    参考文献 (8)

    ab183596 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab183596 は 8 報の論文で使用されています。

    • Luo F  et al. Hepatic TM6SF2 Is Required for Lipidation of VLDL in a Pre-Golgi Compartment in Mice and Rats. Cell Mol Gastroenterol Hepatol 13:879-899 (2022). PubMed: 34923175
    • Fote GM  et al. Isoform-dependent lysosomal degradation and internalization of apolipoprotein E requires autophagy proteins. J Cell Sci 135:N/A (2022). PubMed: 34982109
    • Sauter M  et al. Protocol to isolate and analyze mouse bone marrow derived dendritic cells (BMDC). STAR Protoc 3:101664 (2022). PubMed: 36097382
    • Sauter M  et al. Apolipoprotein E derived from CD11c+ cells ameliorates atherosclerosis. iScience 25:103677 (2022). PubMed: 35036868
    • Wu Y  et al. Features of Lipid Metabolism in Humanized ApoE Knockin Rat Models. Int J Mol Sci 22:N/A (2021). PubMed: 34361033
    • Bosch-Queralt M  et al. Diet-dependent regulation of TGFβ impairs reparative innate immune responses after demyelination. Nat Metab 3:211-227 (2021). PubMed: 33619376
    • Li Z  et al. In situ apolipoprotein E-enriched corona guides dihydroartemisinin-decorating nanoparticles towards LDLr-mediated tumor-homing chemotherapy. Asian J Pharm Sci 15:482-491 (2020). PubMed: 32952671
    • Gao J  et al. Therapeutic IDOL Reduction Ameliorates Amyloidosis and Improves Cognitive Function in APP/PS1 Mice. Mol Cell Biol 40:N/A (2020). PubMed: 31964754

    レビューと Q&A

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    Immunoprecipitation abreview for Anti-Apolipoprotein E antibody [EPR19378]

     Below Average
    Abreviews
    abreview image
    Application
    Immunoprecipitation
    Sample
    Human Cell lysate - whole cell (Melanoma cell line)
    Total protein in input
    1000 µg
    Immuno-precipitation step
    Protein A/G
    Specification
    Melanoma cell line
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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    投稿 Jun 16 2021

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