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Cardiovascular Lipids / Lipoproteins Lipoproteins/Apolipoproteins
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Anti-Apolipoprotein A I 抗体 [12C8] (ab17278)

  • Datasheet
  • SDS
Submit a review Q&A (1)References (9)

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Abpromise

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Western blot - Anti-Apolipoprotein A I antibody [12C8] (ab17278)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein A I antibody [12C8] (ab17278)

Key features and details

  • Mouse monoclonal [12C8] to Apolipoprotein A I
  • Suitable for: ELISA, WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG1

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産

製品の概要

  • 製品名

    Anti-Apolipoprotein A I antibody [12C8]
    Apolipoprotein A I 一次抗体 製品一覧
  • 製品の詳細

    Mouse monoclonal [12C8] to Apolipoprotein A I
  • 由来種

    Mouse
  • 特異性

    No cross reactivity is seen with human apolipoprotein B.
  • アプリケーション

    適用あり: ELISA, WB, IHC-Pmore details
  • 種交差性

    交差種: Human
  • 免疫原

    Full length native Apolipoprotein A1, isolated from human plasma.

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term.
  • バッファー

    pH: 7.40
    Preservative: 0.097% Sodium azide
    Constituents: 0.0268% PBS, 2.9% Sodium chloride
  • Concentration information loading...
  • 精製度

    Protein G purified
  • ポリ/モノ

    モノクローナル
  • クローン名

    12C8
  • アイソタイプ

    IgG1
  • 軽鎖の種類

    kappa
  • 研究分野

    • Cardiovascular
    • Lipids / Lipoproteins
    • Lipoproteins/Apolipoproteins
    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Other
    • Signal Transduction
    • Metabolism
    • Lipid metabolism
    • Cardiovascular
    • Atherosclerosis
    • Lipoprotein metabolism
    • Cardiovascular
    • Lipids / Lipoproteins
    • Lipoproteins/Apolipoproteins
    • Apolipoproteins
    • Cancer
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    • Metabolism of lipids and lipoproteins
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    • Lipoprotein metabolism
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    • Cancer
    • Metabolism
    • Types of disease
    • Heart disease
    • Kits/ Lysates/ Other
    • Kits
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    • ELISA Kits
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関連製品

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • Recombinant Protein

    • Recombinant Human Apolipoprotein A I (ab50239)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab17278の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ELISA
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 24 kDa.
IHC-P
Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
特記事項
ELISA
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 24 kDa.
IHC-P
Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

ターゲット情報

  • 機能

    Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility.
  • 組織特異性

    Major protein of plasma HDL, also found in chylomicrons. Synthesized in the liver and small intestine.
  • 関連疾患

    Defects in APOA1 are a cause of high density lipoprotein deficiency type 2 (HDLD2) [MIM:604091]; also known as familial hypoalphalipoproteinemia (FHA). Inheritance is autosomal dominant.
    Defects in APOA1 are a cause of the low HDL levels observed in high density lipoprotein deficiency type 1 (HDLD1) [MIM:205400]; also known as analphalipoproteinemia or Tangier disease (TGD). HDLD1 is a recessive disorder characterized by the absence of plasma HDL, accumulation of cholesteryl esters, premature coronary artery disease, hepatosplenomegaly, recurrent peripheral neuropathy and progressive muscle wasting and weakness. In HDLD1 patients, ApoA-I fails to associate with HDL probably because of the faulty conversion of pro-ApoA-I molecules into mature chains, either due to a defect in the converting enzyme activity or a specific structural defect in Tangier ApoA-I.
    Defects in APOA1 are the cause of amyloid polyneuropathy-nephropathy Iowa type (AMYLIOWA) [MIM:107680]; also known as amyloidosis van Allen type or familial amyloid polyneuropathy type III. AMYLIOWA is a hereditary generalized amyloidosis due to deposition of amyloid mainly constituted by apolipoprotein A1. The clinical picture is dominated by neuropathy in the early stages of the disease and nephropathy late in the course. Death is due in most cases to renal amyloidosis. Severe peptic ulcer disease can occurr in some and hearing loss is frequent. Cataracts is present in several, but vitreous opacities are not observed.
    Defects in APOA1 are a cause of amyloidosis type 8 (AMYL8) [MIM:105200]; also known as systemic non-neuropathic amyloidosis or Ostertag-type amyloidosis. AMYL8 is a hereditary generalized amyloidosis due to deposition of apolipoprotein A1, fibrinogen and lysozyme amyloids. Viscera are particularly affected. There is no involvement of the nervous system. Clinical features include renal amyloidosis resulting in nephrotic syndrome, arterial hypertension, hepatosplenomegaly, cholestasis, petechial skin rash.
  • 配列類似性

    Belongs to the apolipoprotein A1/A4/E family.
  • 翻訳後修飾

    Palmitoylated.
    Phosphorylation sites are present in the extracelllular medium.
  • 細胞内局在

    Secreted.
  • Target information above from: UniProt accession P02647 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 335 Human
    • Omim: 107680 Human
    • SwissProt: P02647 Human
    • Unigene: 93194 Human
    • 別名

      • Apo-AI antibody
      • ApoA I antibody
      • ApoA-I antibody
      • APOA1 antibody
      • APOA1_HUMAN antibody
      • Apolipoprotein A-I(1-242) antibody
      • Apolipoprotein A1 antibody
      • Apolipoprotein AI antibody
      • Apolipoprotein of high density lipoprotein antibody
      • ApolipoproteinAI antibody
      • Brp14 antibody
      • high density lipoprotein uptake antibody
      • Ltw1 antibody
      • Lvtw1 antibody
      • MGC117399 antibody
      • Sep1 antibody
      • Sep2 antibody
      see all

    画像

    • Western blot - Anti-Apolipoprotein A I antibody [12C8] (ab17278)
      Western blot - Anti-Apolipoprotein A I antibody [12C8] (ab17278)
      All lanes : Anti-Apolipoprotein A I antibody [12C8] (ab17278) at 5 µg/ml

      Lane 1 : Human ovary tissue lysate - total protein (ab30222)
      Lane 2 : Lung (Human) Tissue Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Lane 1 : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
      Lane 2 : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Predicted band size: 24 kDa
      Observed band size: 25 kDa why is the actual band size different from the predicted?
      Additional bands at: 260 kDa, 45 kDa, 55 kDa, 75 kDa. We are unsure as to the identity of these extra bands.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein A I antibody [12C8] (ab17278)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein A I antibody [12C8] (ab17278)
      Ab17278 staining human normal skeletal muscle. Staining is localised to the cytoplasm.
      Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
      Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS), then incubated with primary antibody for 20 minutes, and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

    プロトコール

    • Western blot protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    データシートおよび資料

    • SDS download

    • Datasheet download

      Download

    参考文献 (9)

    ab17278 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab17278 は 9 報の論文で使用されています。

    • Gori A  et al. Membrane-binding peptides for extracellular vesicles on-chip analysis. J Extracell Vesicles 9:1751428 (2020). PubMed: 32363015
    • Garg PK  et al. Cholesterol mass efflux capacity and risk of peripheral artery disease: The Multi-Ethnic Study of Atherosclerosis. Atherosclerosis 297:81-86 (2020). PubMed: 32097805
    • Zocco D  et al. Isolation of extracellular vesicles improves the detection of mutant DNA from plasma of metastatic melanoma patients. Sci Rep 10:15745 (2020). PubMed: 32978468
    • Cortelazzo A  et al. Expression and oxidative modifications of plasma proteins in autism spectrum disorders: Interplay between inflammatory response and lipid peroxidation. Proteomics Clin Appl 10:1103-1112 (2016). PubMed: 27246309
    • Amjadi F  et al. Apolipoprotein A1 as a novel anti-implantation biomarker in polycystic ovary syndrome: A case-control study. J Res Med Sci 20:1039-45 (2015). WB, IHC . PubMed: 26941806
    • Johnson LA  et al. Diabetic atherosclerosis in APOE*4 mice: synergy between lipoprotein metabolism and vascular inflammation. J Lipid Res 54:386-96 (2013). Mouse . PubMed: 23204275
    • Yvan-Charvet L  et al. Cholesterol efflux potential and antiinflammatory properties of high-density lipoprotein after treatment with niacin or anacetrapib. Arterioscler Thromb Vasc Biol 30:1430-8 (2010). PubMed: 20448206
    • Yvan-Charvet L  et al. Inhibition of cholesteryl ester transfer protein by torcetrapib modestly increases macrophage cholesterol efflux to HDL. Arterioscler Thromb Vasc Biol 27:1132-8 (2007). PubMed: 17322101
    • Matsuura F  et al. HDL from CETP-deficient subjects shows enhanced ability to promote cholesterol efflux from macrophages in an apoE- and ABCG1-dependent pathway. J Clin Invest 116:1435-42 (2006). PubMed: 16670775

    レビューと Q&A

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    レビューを送る 質問を送る

    Question

    Product code: 17278
    Lot number: GR83915-1

    Can you provide a Certificate of Analysis for this product?

    Read More

    Abcam community

    Verified customer

    Asked on Sep 04 2012

    Answer

    Thank you for contacting us.

    The Certificate of Compliance for ab17278, lot GR83915 is attached to this email.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Abcam Scientific Support

    Answered on Sep 04 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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