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リコンビナントRabMAb

Anti-APOL3 抗体 [EPR8238(2)] (ab154869)

Submit a review Submit a question References (1)
Western blot - Anti-APOL3 antibody [EPR8238(2)] (ab154869)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOL3 antibody [EPR8238(2)] (ab154869)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOL3 antibody [EPR8238(2)] (ab154869)
  • Anti-APOL3 antibody [EPR8238(2)] (ab154869)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8238(2)] to APOL3
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-APOL3 antibody [EPR8238(2)]
    APOL3 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR8238(2)] to APOL3

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-Pmore details
    適用なし: ICC/IF or IP

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • Fetal kidney, Jurkat and Daudi lysates; Human colon and kidney tissues.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at -20ºC.

  • バッファー

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR8238(2)

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab154869の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000 - 1/10000. Predicted molecular weight: 44 kDa.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
特記事項
WB
1/1000 - 1/10000. Predicted molecular weight: 44 kDa.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
追加情報
Is unsuitable for ICC/IF or IP.

ターゲット情報

  • 機能

    May affect the movement of lipids in the cytoplasm or allow the binding of lipids to organelles.

  • 組織特異性

    Widely expressed; the highest levels are in prostate, lung and placenta; also detected in kidney, bone marrow, spleen, thymus, spinal cord, adrenal gland, salivary gland, trachea and mammary gland; levels are low in brain, heart, fetal liver, pancreas and testis.

  • 配列類似性

    Belongs to the apolipoprotein L family.

  • 細胞内局在

    Cytoplasm.
  • Information by UniProt

  • 参照データベース

    • 別名

      • ApoL III antibody
      • ApoL-III antibody
      • APOL3 antibody
      • APOL3_HUMAN antibody
      • APOLIII antibody
      • Apolipoprotein L 3 antibody
      • Apolipoprotein L III antibody
      • Apolipoprotein L-III antibody
      • Apolipoprotein L3 antibody
      • Apolipoprotein LIII antibody
      • CG12 1 antibody
      • CG12_1 antibody
      • CG121 antibody
      • TNF inducible protein CG12 1 antibody
      • TNF-inducible protein CG12-1 antibody
      see all

    画像

    • Western blot - Anti-APOL3 antibody [EPR8238(2)] (ab154869)
      Western blot - Anti-APOL3 antibody [EPR8238(2)] (ab154869)
      All lanes : Anti-APOL3 antibody [EPR8238(2)] (ab154869) at 1/1000 dilution

      Lane 1 : Fetal kidney tissue lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : Daudi cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 44 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOL3 antibody [EPR8238(2)] (ab154869)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOL3 antibody [EPR8238(2)] (ab154869)

      Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling APOL3 with ab154869 at 1/100 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOL3 antibody [EPR8238(2)] (ab154869)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APOL3 antibody [EPR8238(2)] (ab154869)

      Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling APOL3 with ab154869 at 1/100 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Anti-APOL3 antibody [EPR8238(2)] (ab154869)
      Anti-APOL3 antibody [EPR8238(2)] (ab154869)

    参考文献 (1)

    ab154869 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab154869 は 1 報の論文で使用されています。

    • Rosain J  et al. Human IRF1 governs macrophagic IFN-γ immunity to mycobacteria. Cell 186:621-645.e33 (2023). PubMed: 36736301

    レビューと Q&A

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