Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
遺伝子ノックアウト細胞株 検証済リコンビナントRabMAb

Anti-ApoER2 抗体 [EPR3326] (ab108208)

Reviews (1) Submit a question References (24)
Western blot - Anti-ApoER2 antibody [EPR3326] (ab108208)
  • Immunoprecipitation - Anti-ApoER2 antibody [EPR3326] (ab108208)
  • Western blot - Anti-ApoER2 antibody [EPR3326] (ab108208)
  • Western blot - Anti-ApoER2 antibody [EPR3326] (ab108208)
  • Anti-ApoER2 antibody [EPR3326] (ab108208)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3326] to ApoER2
  • Suitable for: WB, IP
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-ApoER2 antibody [EPR3326]
    ApoER2 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR3326] to ApoER2

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IPmore details
    適用なし: ICC/IF or IHC-P

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • SH-SY5Y, C6, Neuro-2a, Human fetal brain, Mouse brain, and Rat brain lysates,

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab108208の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (1)
1/1000 - 1/10000. Predicted molecular weight: 106 kDa.
IP
1/10 - 1/100.
特記事項
WB
1/1000 - 1/10000. Predicted molecular weight: 106 kDa.
IP
1/10 - 1/100.
追加情報
Is unsuitable for ICC/IF or IHC-P.

ターゲット情報

  • 機能

    Cell surface receptor for Reelin (RELN) and apolipoprotein E (apoE)-containing ligands. LRP8 participates in transmitting the extracellular Reelin signal to intracellular signaling processes, by binding to DAB1 on its cytoplasmic tail. Reelin acts via both the VLDL receptor (VLDLR) and LRP8 to regulate DAB1 tyrosine phosphorylation and microtubule function in neurons. LRP8 has higher affinity for Reelin than VLDLR. LRP8 is thus a key component of the Reelin pathway which governs neuronal layering of the forebrain during embryonic brain development. Binds the endoplasmic reticulum resident receptor-associated protein (RAP). Binds dimers of beta 2-glycoprotein I and may be involved in the suppression of platelet aggregation in the vasculature. Highly expressed in the initial segment of the epididymis, where it affects the functional expression of clusterin and phospholipid hydroperoxide glutathione peroxidase (PHGPx), two proteins required for sperm maturation. May also function as an endocytic receptor.

  • 組織特異性

    Expressed mainly in brain and placenta. Also expressed in platelets and megakaryocytic cells. Not expressed in the liver.

  • 関連疾患

    Defects in LRP8 are a cause of myocardial infarction type 1 (MCI1) [MIM:608446]. A condition defined by the irreversible necrosis of heart muscle secondary to prolonged ischemia.

  • 配列類似性

    Belongs to the LDLR family.
    Contains 2 EGF-like domains.
    Contains 7 LDL-receptor class A domains.
    Contains 5 LDL-receptor class B repeats.

  • ドメイン

    The cytoplasmic domain is involved in the binding of DAB1 and in the recruitment of JNK-interacting proteins. Isoforms, which lack part of the cytoplasmic domain, are unable to recruit members of the family of JNK interacting proteins (JIP) to the cytoplasmic tail.

  • 翻訳後修飾

    O-glycosylated. Some alternatively spliced isoforms lack the O-linked sugar domain.
    Undergoes sequential, furin and gamma-secretase dependent, proteolytic processing, resulting in the extracellular release of the entire ligand-binding domain as a soluble polypeptide and in the intracellular domain (ICD) release into the cytoplasm. The gamma-secretase-dependent proteolytical processing occurs after the bulk of the extracellular domain has been shed, in a furin-dependent manner, in alternatively spliced isoforms carrying the furin cleavage site. Hypoglycosylation (mainly hypo-O-glycosylation) leads to increased extracellular cleavage, which in turn results in accelerating release of the intracellular domain (ICD) by the gamma-secretase. The resulting receptor fragment is able to inhibit Reelin signaling and in particular the Reelin-induced DAB1 phosphorylation.
    Tyrosine phosphorylated upon apoE binding.
    Ubiquitinated by MYLIP leading to degradation.

  • 細胞内局在

    Cell membrane. Secreted. Isoforms that contain the exon coding for a furin-type cleavage site are proteolytically processed, leading to a secreted receptor fragment.
  • Information by UniProt

  • 参照データベース

    • 別名

      • APOER2 antibody
      • Apolipoprotein E receptor 2 antibody
      • low density lipoprotein receptor-related protein 8 antibody
      • Low-density lipoprotein receptor-related protein 8 antibody
      • LRP-8 antibody
      • LRP8 antibody
      • LRP8_HUMAN antibody
      see all

    画像

    • Western blot - Anti-ApoER2 antibody [EPR3326] (ab108208)
      Western blot - Anti-ApoER2 antibody [EPR3326] (ab108208)
      All lanes : Anti-ApoER2 antibody [EPR3326] (ab108208) at 1/1000 dilution

      Lane 1 : Wild-type HAP1 whole cell lysate
      Lane 2 : ApoER2 knockout HAP1 whole cell lysate

      Lysates/proteins at 40 µg per lane.

      Predicted band size: 106 kDa



      Lanes 1 - 2: Merged signal (red and green). Green - ab108208 observed at 106 kDa. Red - loading control, ab9484, observed at 37 kDa.

      ab108208 was shown to specifically react with ApoER2 in wild-type HAP1 cells as signal was lost in LRP8 knockout cells. Wild-type and ApoER2 knockout samples were subjected to SDS-PAGE. Ab108208 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    • Immunoprecipitation - Anti-ApoER2 antibody [EPR3326] (ab108208)
      Immunoprecipitation - Anti-ApoER2 antibody [EPR3326] (ab108208)

      ab108208 (purified) at 1/40 immunoprecipitating ApoER2 in SH-SY5Y (human neuroblastoma) whole cell lysate.

      Lane 1 (input): SH-SY5Y whole cell lysate 10ug

      Lane 2 (+): ab108208 + SH-SY5Y whole cell lysate

      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab108208 in SH-SY5Y whole cell lysate

      For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.

      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • Western blot - Anti-ApoER2 antibody [EPR3326] (ab108208)
      Western blot - Anti-ApoER2 antibody [EPR3326] (ab108208)
      All lanes : Anti-ApoER2 antibody [EPR3326] (ab108208) at 1/10000 dilution (purified)

      Lane 1 : SH-SY5Y (human neuroblastoma) whole cell lysate
      Lane 2 : Human fetal brain tissue lysate
      Lane 3 : Mouse brain tissue lysate
      Lane 4 : C6 (rat glioma) whole cell lysate
      Lane 5 : Neuro-2a (mouse neuroblastoma) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

      Predicted band size: 106 kDa
      Observed band size: 130-170 kDa why is the actual band size different from the predicted?



      Blocking and diluting buffer 5% NFDM/TBST.

      130kDa: Immature form;

      170kDa: Glycosylated mature form. (PMID:24344333, 24532792, 24705369 and 25233900)

    • Western blot - Anti-ApoER2 antibody [EPR3326] (ab108208)
      Western blot - Anti-ApoER2 antibody [EPR3326] (ab108208)
      All lanes : Anti-ApoER2 antibody [EPR3326] (ab108208) at 1/1000 dilution (unpurified)

      Lane 1 : SH-SY5Y cell lysate
      Lane 2 : C6 cell lysate
      Lane 3 : Neuro-2a cell lysate
      Lane 4 : Human Fetal Brain cell lysate
      Lane 5 : Mouse Fetal Brain cell lysate
      Lane 6 : Rat Fetal Brain cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 106 kDa

    • Anti-ApoER2 antibody [EPR3326] (ab108208)
      Anti-ApoER2 antibody [EPR3326] (ab108208)

    参考文献 (24)

    ab108208 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab108208 は 24 報の論文で使用されています。

    • Alam SB  et al. Apolipoprotein C3 facilitates internalization of cationic lipid nanoparticles into bone marrow-derived mouse mast cells. Sci Rep 13:431 (2023). PubMed: 36624108
    • Greenough MA  et al. Selective ferroptosis vulnerability due to familial Alzheimer's disease presenilin mutations. Cell Death Differ 29:2123-2136 (2022). PubMed: 35449212
    • Li Z  et al. Ribosome stalling during selenoprotein translation exposes a ferroptosis vulnerability. Nat Chem Biol 18:751-761 (2022). PubMed: 35637349
    • Adjei-Sowah EA  et al. Investigating the Interactions of Glioma Stem Cells in the Perivascular Niche at Single-Cell Resolution using a Microfluidic Tumor Microenvironment Model. Adv Sci (Weinh) 9:e2201436 (2022). PubMed: 35619544
    • Komata Y  et al. Screening of novel Midkine binding protein by BioID2-based proximity labeling. Nagoya J Med Sci 83:495-508 (2021). PubMed: 34552285
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    Western blot abreview for Anti-ApoER2 antibody [EPR3326]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Human THP-1 cell line, various mouse tissues)
    Loading amount
    15 µg
    Specification
    Human THP-1 cell line, various mouse tissues
    Gel Running Conditions
    Reduced Denaturing (4-12% Bis-Tris gel (Novex))
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Nov 02 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com