Anti-APOBEC3B 抗体 [EPR18138] (ab184990)
![Western blot - Anti-APOBEC3B antibody [EPR18138] (ab184990)](https://www.abcam.co.jp/ps/products/184/ab184990/Images/ab184990-490674-anti-apobec3b-antibody-epr18138-western-blot-human.jpg "Western blot - Anti-APOBEC3B antibody [EPR18138] (ab184990)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18138] to APOBEC3B
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-APOBEC3B antibody [EPR18138]
APOBEC3B 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR18138] to APOBEC3B -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- WB: Human APOBEC3B fragment recombinant protein, HepaRG-APOBEC3B tetracycline-inducible cell line, HCC1143 cell line infected with control (+), APOBEC3B lentivirus (-), HEK293-APOBEC3B(+GFP) tetracycline-inducible cell line and A431 whole cell lysate.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR18138 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab184990の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (2) |
1/500 - 1/10000. Detects a band of approximately 35,38,46,70 kDa (predicted molecular weight: 46 kDa).
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| 特記事項 |
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WB
1/500 - 1/10000. Detects a band of approximately 35,38,46,70 kDa (predicted molecular weight: 46 kDa). |
ターゲット情報
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関連性
APOBEC3B is a member of the cytidine deaminase gene family. However, it lacks cytidine deaminase activity, at least on RNA molecules (monomeric nucleoside substrates or synthetic apoB, NF1 and NAT1 RNA template). It binds to apoB and AU-rich RNAs. It is unable to reduce HIV-1 infectivity in vitro. -
細胞内局在
Nuclear -
参照データベース
- Entrez Gene: 9582 Human
- Omim: 607110 Human
- SwissProt: Q9UH17 Human
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別名
- A3B antibody
- APOBEC1L antibody
- Apolipoprotein B mRNA editing enzyme catalytic polypeptide like 3B antibody
see all
画像
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Western blot - Anti-APOBEC3B antibody [EPR18138] (ab184990)Anti-APOBEC3B antibody [EPR18138] (ab184990) at 1/500 dilution + A431 (Human epidermoid carcinoma epithelial cell) whole cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 46 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted?
Exposure time: 180 secondsBlocking buffer and concentration: 5% NFDM/TBST
Diluting buffer and concentration: 5% NFDM /TBSTWe are unsure of the identity of the 55kda band.
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![Western blot - Anti-APOBEC3B antibody [EPR18138] (ab184990)](https://www.abcam.co.jp/ps/products/184/ab184990/Images/ab184990-253254-anti-apobec3b-antibody-epr18138-western-blot.jpg)
Western blot - Anti-APOBEC3B antibody [EPR18138] (ab184990)All lanes : Anti-APOBEC3B antibody [EPR18138] (ab184990) at 1/1000 dilution
Lane 1 : Human APOBEC3B fragment recombinant protein
Lane 2 : Human APOBEC3G fragment recombinant protein
Lysates/proteins at 0.01 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution
Predicted band size: 46 kDa
Observed band size: 46 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-APOBEC3B antibody [EPR18138] (ab184990)](https://www.abcam.co.jp/ps/products/184/ab184990/Images/ab184990-253249-anti-apobec3b-antibody-epr18138-western-blot.jpg)
Western blot - Anti-APOBEC3B antibody [EPR18138] (ab184990)All lanes : Anti-APOBEC3B antibody [EPR18138] (ab184990) at 1/10000 dilution
Lane 1 : HepaRG-APOBEC3B tetracycline-inducible cell line (+/-)
Lane 2 : HCC1143 cell line infected with control (+) and APOBEC3B lentivirus (-)
Lane 3 : HEK293-APOBEC3B(+GFP) tetracycline-inducible cell line (+/-)
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (H+L) conjugated to HRP at 1/10000 dilution
Predicted band size: 46 kDa
Observed band size: 35,70 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST.
Dilution buffer: 1% NFDM/TBST.
Exposure time: Panel A: 20 s; Panel B: 1 min.
Figure provided by Dr. Jenni Nikkila. Gene Function Lab at The Institute of Cancer Research, London. HEK293-APOBEC3B(+GFP) tetracycline-inducible cell line provide by Dr Reuben Harris, University of Minnesota, Minneapolis - cited in Akre, M.K, G.J. Starrett, J.S. Quist, N.A. Temiz, M.A. Carpenter, A.N.J. Tutt, A. Grigoriadis & R.S. Harris (2016) Mutation processes in 293-based clones overexpressing the DNA cytosine deaminase APOBEC3B. PLoS One11(5):e0155391. doi: 10.1371/journal.pone.0155391.
The 35 kDa band represents a known splice variant of APOBEC3B.
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![Anti-APOBEC3B antibody [EPR18138] (ab184990)](https://www.abcam.co.jp/ps/products/184/ab184990/Images/ab184990-7-benefits-of-recombinant-antibodies.png)
Anti-APOBEC3B antibody [EPR18138] (ab184990)
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (14)
ab184990 は 14 報の論文で使用されています。
- Petljak M et al. Mechanisms of APOBEC3 mutagenesis in human cancer cells. Nature 607:799-807 (2022). PubMed: 35859169
- Shi Z et al. CircNRIP1 Encapsulated by Bone Marrow Mesenchymal Stem Cell-Derived Extracellular Vesicles Aggravates Osteosarcoma by Modulating the miR-532-3p/AKT3/PI3K/AKT Axis. Front Oncol 11:658139 (2021). PubMed: 34660257
- Bandarra S et al. APOBEC3B Potently Restricts HIV-2 but Not HIV-1 in a Vif-Dependent Manner. J Virol 95:e0117021 (2021). PubMed: 34523960
- Bader SB et al. Replication catastrophe induced by cyclic hypoxia leads to increased APOBEC3B activity. Nucleic Acids Res 49:7492-7506 (2021). PubMed: 34197599
- Kurashima K et al. SMARCA4 deficiency-associated heterochromatin induces intrinsic DNA replication stress and susceptibility to ATR inhibition in lung adenocarcinoma. NAR Cancer 2:zcaa005 (2020). PubMed: 34316685