Anti-APOA1 抗体 [EPR25446-9] (BSA and Azide free) (ab300086)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR25446-9] to APOA1 - ChIP Grade – BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse
Related conjugates and formulations
製品の概要
-
製品名
Anti-APOA1 antibody [EPR25446-9] (BSA and Azide free)
APOA1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR25446-9] to APOA1 - ChIP Grade – BSA and Azide free -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details
適用なし: IHC-P or IP -
種交差性
交差種: Mouse
非交差種: Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
ポジティブ・コントロール
- WB: mouse plasma, and mouse: lung, brain, kidney tissues lysates.
-
特記事項
ab300086 is a carrier free version of ab300085.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at +4°C. -
バッファー
pH: 7.2
Constituent: 100% PBS -
キャリア・フリー
はい -
Concentration information loading...
-
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR25446-9 -
アイソタイプ
IgG
関連製品
-
Alternative Versions
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab300086の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
Use at an assay dependent concentration.
|
特記事項 |
---|
WB
Use at an assay dependent concentration. |
画像
-
All lanes : Anti-APOA1 antibody [EPR25446-9] (ab300085) at 1/1000 dilution
Lane 1 : Mouse plasma
Lane 2 : Mouse lung tissue lysate
Lane 3 : Mouse brain tissue lysate
Lane 4 : Mouse kidney tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Observed band size: 28 kDa why is the actual band size different from the predicted?
Exposure time: 15 secondsThis data was developed using ab300085, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration were 5% NFDM/TBST.
The expression profile/molecular weight observed is consistent with that described in the literature (PMID: 23969698, 23409054).
Low expression: brain (PMID: 1478650)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
-
Datasheet download
Certificate of Compliance
参考文献 (0)
ab300086 は論文での使用が確認できていません。