Anti-APOA1 抗体 [EPR25446-9] (ab300085)
![Western blot - Anti-APOA1 antibody [EPR25446-9] (AB300085)](https://www.abcam.co.jp/ps/products/300/ab300085/Images/AB300085-485238-25446WB3.jpg "Western blot - Anti-APOA1 antibody [EPR25446-9] (AB300085)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR25446-9] to APOA1 - ChIP Grade
- Suitable for: WB
- Reacts with: Mouse
Related conjugates and formulations
製品の概要
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製品名
Anti-APOA1 antibody [EPR25446-9]
APOA1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR25446-9] to APOA1 - ChIP Grade -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details
適用なし: IHC-P or IP -
種交差性
交差種: Mouse
非交差種: Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- WB: mouse plasma, and mouse: lung, brain, kidney tissues lysates.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR25446-9 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab300085の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 28 kDa (predicted molecular weight: 30 kDa).
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| 特記事項 |
|---|
|
WB
1/1000. Detects a band of approximately 28 kDa (predicted molecular weight: 30 kDa). |
画像
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Western blot - Anti-APOA1 antibody [EPR25446-9] (AB300085)All lanes : Anti-APOA1 antibody [EPR25446-9] (ab300085) at 1/1000 dilution
Lane 1 : Mouse plasma
Lane 2 : Mouse lung tissue lysate
Lane 3 : Mouse brain tissue lysate
Lane 4 : Mouse kidney tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 30 kDa
Observed band size: 28 kDa why is the actual band size different from the predicted?
Exposure time: 15 secondsBlocking and diluting buffer and concentration were 5% NFDM/TBST.
The expression profile/molecular weight observed is consistent with that described in the literature (PMID: 23969698, 23409054).
Low expression: brain (PMID: 1478650) -
![Anti-APOA1 antibody [EPR25446-9] (AB300085)](https://www.abcam.co.jp/ps/products/300/ab300085/Images/AB300085-485244-Benefits-of-Recombinant-antibodies.jpg)
Anti-APOA1 antibody [EPR25446-9] (AB300085)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab300085 は論文での使用が確認できていません。