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リコンビナントRabMAb

Anti-APLP1 抗体 [EPR25737-10] (BSA and Azide free) (ab291097)

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Western blot - Anti-APLP1 antibody [EPR25737-10] - BSA and Azide free (ab291097)
  • Western blot - Anti-APLP1 antibody [EPR25737-10] - BSA and Azide free (ab291097)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APLP1 antibody [EPR25737-10] - BSA and Azide free (ab291097)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APLP1 antibody [EPR25737-10] - BSA and Azide free (ab291097)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APLP1 antibody [EPR25737-10] - BSA and Azide free (ab291097)
  • Flow Cytometry - Anti-APLP1 antibody [EPR25737-10] - BSA and Azide free (ab291097)
  • Immunoprecipitation - Anti-APLP1 antibody [EPR25737-10] - BSA and Azide free (ab291097)
  • Immunoprecipitation - Anti-APLP1 antibody [EPR25737-10] - BSA and Azide free (ab291097)
  • Anti-APLP1 antibody [EPR25737-10] - BSA and Azide free (ab291097)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR25737-10] to APLP1 - BSA and Azide free
  • Suitable for: WB, IP, Flow Cyt, IHC-P
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Unconjugated

製品の概要

  • 製品名

    Anti-APLP1 antibody [EPR25737-10] (BSA and Azide free)
    APLP1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR25737-10] to APLP1 - BSA and Azide free

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IP, Flow Cyt, IHC-Pmore details
    適用なし: ICC/IF or IHC-Fr

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Human tissue lysates of: cerebellum, liver, intestine, colon cancer; Mouse tissue lysates of: brain, brain treated with PNGase F, cerebral cortex, liver; Rat tissue lysates: brain, cerebral cortex, liver. IHC-P: Human, mouse and rat cerebrum. Flow cyt.: mouse primary neuron cell IP: SH-SY5Y whole cell lysate, mouse brain tissue lysate.

  • 特記事項

    ab291097 is a carrier free version of ab291070. 

    This antibody does not react with Human species for FC application.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab291097の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
Use at an assay dependent concentration. Predicted molecular weight: 72 kDa.
IP
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
特記事項
WB
Use at an assay dependent concentration. Predicted molecular weight: 72 kDa.
IP
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
追加情報
Is unsuitable for ICC/IF or IHC-Fr.

ターゲット情報

  • 機能

    May play a role in postsynaptic function. The C-terminal gamma-secretase processed fragment, ALID1, activates transcription activation through APBB1 (Fe65) binding (By similarity). Couples to JIP signal transduction through C-terminal binding. May interact with cellular G-protein signaling pathways. Can regulate neurite outgrowth through binding to components of the extracellular matrix such as heparin and collagen I.
    The gamma-CTF peptide, C30, is a potent enhancer of neuronal apoptosis.

  • 組織特異性

    Expressed in the cerebral cortex where it is localized to the postsynaptic density (PSD).

  • 配列類似性

    Belongs to the APP family.

  • ドメイン

    The NPXY sequence motif found in many tyrosine-phosphorylated proteins is required for the specific binding of the PID domain. However, additional amino acids either N- or C-terminal to the NPXY motif are often required for complete interaction. The NPXY site is also involved in clathrin-mediated endocytosis.

  • 翻訳後修飾

    Proteolytically cleaved by caspases during neuronal apoptosis. Cleaved, in vitro, at Asp-620 by caspase-3.
    N- and O-glycosylated. O-glycosylation with core 1 or possibly core 8 glycans. Glycosylation on Ser-227 is the preferred site to Thr-228.

  • 細胞内局在

    Cell membrane and Cytoplasm. C-terminally processed in the Golgi complex.
  • Information by UniProt

  • 参照データベース

    • 別名

      • AMYLOID BETA A4 PRECURSOR-LIKE PROTEIN 1 antibody
      • AMYLOID PRECURSOR-LIKE PROTEIN antibody
      • Amyloid-like protein 1 precursor antibody
      • APLP 1 antibody
      • APLP antibody
      • APLP-1 antibody
      • Aplp1 antibody
      • APLP1_HUMAN antibody
      • C30 antibody
      see all

    画像

    • Western blot - Anti-APLP1 antibody [EPR25737-10] - BSA and Azide free (ab291097)
      Western blot - Anti-APLP1 antibody [EPR25737-10] - BSA and Azide free (ab291097)
      All lanes : Anti-APLP1 antibody [EPR25737-10] (ab291070) at 1/1000 dilution

      Lane 1 : Human cerebellum tissue lysate
      Lane 2 : Human liver tissue lysate
      Lane 3 : Human small intestine tissue lysate
      Lane 4 : Human colon cancer tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 72 kDa
      Observed band size: 97 kDa why is the actual band size different from the predicted?


      Exposure time: 26 seconds


      This data was developed using ab291070, the same antibody clone in a different buffer formulation. 

      Blocking and dilution buffer and concentration: 5% NFDM/TBST. 

      Samples are non-boiled as boiling may cause protein aggregates.
      The expression profile/molecular weight observed is consistent with that described in the literature (PMID: 7494461; PMID: 18430897).
      Low expression: Liver, small intestine, colon cancer (PMID:18430897). 

    • Western blot - Anti-APLP1 antibody [EPR25737-10] - BSA and Azide free (ab291097)
      Western blot - Anti-APLP1 antibody [EPR25737-10] - BSA and Azide free (ab291097)
      All lanes : Anti-APLP1 antibody [EPR25737-10] (ab291070) at 1/1000 dilution

      Lanes 1 & 7 : Mouse brain tissue lysate
      Lane 2 : Mouse cerebral cortex tissue lysate
      Lane 3 : Mouse liver tissue lysate
      Lane 4 : Rat brain tissue lysate
      Lane 5 : Rat cerebral cortex tissue lysate
      Lane 6 : Rat liver tissue lysate
      Lane 8 : Mouse brain tissue lysate treated with PNGase F

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 72 kDa
      Observed band size: 72, 97 kDa why is the actual band size different from the predicted?


      Exposure time: 26 seconds


      This data was developed using ab291070, the same antibody clone in a different buffer formulation. 

      Blocking and dilution buffer and concentration: 5% NFDM/TBST. 

      Samples are non-boiled as boiling may cause protein aggregates.
      The expression profile/molecular weight observed is consistent with that described in the literature (PMID: 7494461; PMID: 18430897).
      Low expression: Liver (PMID: 18430897 ). 

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APLP1 antibody [EPR25737-10] - BSA and Azide free (ab291097)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APLP1 antibody [EPR25737-10] - BSA and Azide free (ab291097)

      This data was developed using ab291070, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labelling APLP1 with ab291070 at 1/5000, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human cerebrum (PMID: 9444352). The section was incubated with ab291070 for 10 mins at room temperature.
      The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: PBS was used instead of primary antibody followed by ready to use secondary antibody LeicaDS9800 (Bond™ Polymer Refine Detection). 

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins was used. 

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APLP1 antibody [EPR25737-10] - BSA and Azide free (ab291097)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APLP1 antibody [EPR25737-10] - BSA and Azide free (ab291097)

      This data was developed using ab291070, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labelling APLP1 with ab291070 at 1/5000, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse cerebrum. The section was incubated with ab291070 for 10 mins at room temperature.
      The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: PBS was used instead of primary antibody followed by ready to use secondary antibody LeicaDS9800 (Bond™ Polymer Refine Detection). 

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins was used. 

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APLP1 antibody [EPR25737-10] - BSA and Azide free (ab291097)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APLP1 antibody [EPR25737-10] - BSA and Azide free (ab291097)

      This data was developed using ab291070, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labelling APLP1 with ab291070 at 1/5000, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on rat cerebrum. The section was incubated with ab291070 for 10 mins at room temperature.
      The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: PBS was used instead of primary antibody followed by ready to use secondary antibody LeicaDS9800 (Bond™ Polymer Refine Detection). 

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins was used. 

    • Flow Cytometry - Anti-APLP1 antibody [EPR25737-10] - BSA and Azide free (ab291097)
      Flow Cytometry - Anti-APLP1 antibody [EPR25737-10] - BSA and Azide free (ab291097)

      This data was developed using ab291070, the same antibody clone in a different buffer formulation. 

      Flow cytometric analysis of mouse primary neuron cells labelling APLP1 with Ab291070 at 1/50 dilution compared with a rabbit monoclonal IgG (ab172730) / Left isotype control. Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) was used as the secondary antibody. 

      Gated on viable cells.

    • Immunoprecipitation - Anti-APLP1 antibody [EPR25737-10] - BSA and Azide free (ab291097)
      Immunoprecipitation - Anti-APLP1 antibody [EPR25737-10] - BSA and Azide free (ab291097)

      This data was developed using ab291070, the same antibody clone in a different buffer formulation. 

      APLP1 was immunoprecipitated from 0.35 mg SH-SY5Y (human neuroblastoma epithelial cell), whole cell lysate with ab291070 at 1/30 dilution. Western blot was performed on the immunoprecipitate using ab291070 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

      Lane 1 (Input): SH-SY5Y (human neuroblastoma epithelial cell), whole cell lysate 10 μg

      Lane 2 (+): SH-SY5Y whole cell lysate

      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab291070 in SH-SY5Y whole cell lysate

      Blocking and dilution buffer and concentration: 5% NFDM/TBST. 

      Exposure time: 75 seconds

    • Immunoprecipitation - Anti-APLP1 antibody [EPR25737-10] - BSA and Azide free (ab291097)
      Immunoprecipitation - Anti-APLP1 antibody [EPR25737-10] - BSA and Azide free (ab291097)

      This data was developed using ab291070, the same antibody clone in a different buffer formulation. 

      APLP1 was immunoprecipitated from 0.35 mg mouse brain tissue lysate 10 μg with ab291070 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab291070 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

      Lane 1 (Input): mouse brain tissue lysate 10 μg

      Lane 2 (+): Mouse brain tissue lysate

      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab291070 in Mouse brain tissue lysate

      Blocking and dilution buffer and concentration: 5% NFDM/TBST. 

      Exposure time: 7.5 seconds

      The bands observed below 97kDa may be the degraded target protein because of long term incubation.

    • Anti-APLP1 antibody [EPR25737-10] - BSA and Azide free (ab291097)
      Anti-APLP1 antibody [EPR25737-10] - BSA and Azide free (ab291097)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab291097 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab291097 は論文での使用が確認できていません。

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