Anti-APLP1 抗体 [EPR25737-10] (ab291070)

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Western blot - Anti-APLP1 antibody [EPR25737-10] (ab291070)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR25737-10] to APLP1
Suitable for: Flow Cyt, IP, IHC-P, WB
Reacts with: Mouse, Rat, Human

Carrier Free

製品名

Anti-APLP1 antibody [EPR25737-10]
APLP1 一次抗体製品一覧
製品の詳細

Rabbit monoclonal [EPR25737-10] to APLP1
由来種

Rabbit
アプリケーション

適用あり: Flow Cyt, IP, IHC-P, WBmore details
適用なし: ICC/IF or IHC-Fr
種交差性

交差種: Mouse, Rat, Human
免疫原

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
ポジティブ･コントロール
- WB: Human cerebellum, Human liver, Human small intestine, Human colon cancer, Mouse brain, Mouse cerebral cortex, Mouse liver, Rat brain, Rat cerebral cortex, Rat liver, Mouse brain and Mouse brain treated with PNGase F lysates. IHC-P: Human cerebrum, Mouse cerebrum and Rat cerebrum tissues. Flow Cyt: Mouse primary neuron cell. IP: SH-SY5Y and Mouse brain cells
特記事項
This antibody does not react with Human species for FC application.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

製品の状態

Liquid
保存方法

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
バッファー

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
精製度

Protein A purified
ポリ/モノ

モノクローナル
クローン名

EPR25737-10
アイソタイプ

IgG
研究分野
- Neuroscience
- Diseases

Alternative Versions
- Anti-APLP1 antibody [EPR25737-10] (BSA and Azide free) (ab291097)
Compatible Secondaries
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

The Abpromise guarantee

Abpromise保証は、次のテスト済みアプリケーションにおけるab291070の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション	Abreviews	特記事項
Flow Cyt		1/50.
IP		1/30.
IHC-P		1/5000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB		1/1000. Predicted molecular weight: 72 kDa.

特記事項
Flow Cyt 1/50.
IP 1/30.
IHC-P 1/5000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/1000. Predicted molecular weight: 72 kDa.

追加情報

Is unsuitable for ICC/IF or IHC-Fr.

機能

May play a role in postsynaptic function. The C-terminal gamma-secretase processed fragment, ALID1, activates transcription activation through APBB1 (Fe65) binding (By similarity). Couples to JIP signal transduction through C-terminal binding. May interact with cellular G-protein signaling pathways. Can regulate neurite outgrowth through binding to components of the extracellular matrix such as heparin and collagen I.
The gamma-CTF peptide, C30, is a potent enhancer of neuronal apoptosis.
組織特異性

Expressed in the cerebral cortex where it is localized to the postsynaptic density (PSD).
配列類似性

Belongs to the APP family.
ドメイン

The NPXY sequence motif found in many tyrosine-phosphorylated proteins is required for the specific binding of the PID domain. However, additional amino acids either N- or C-terminal to the NPXY motif are often required for complete interaction. The NPXY site is also involved in clathrin-mediated endocytosis.
翻訳後修飾

Proteolytically cleaved by caspases during neuronal apoptosis. Cleaved, in vitro, at Asp-620 by caspase-3.
N- and O-glycosylated. O-glycosylation with core 1 or possibly core 8 glycans. Glycosylation on Ser-227 is the preferred site to Thr-228.
細胞内局在

Cell membrane and Cytoplasm. C-terminally processed in the Golgi complex.
Target information above from: UniProt accession P51693 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
参照データベース
- Entrez Gene: 333 Human
- Entrez Gene: 11803 Mouse
- Entrez Gene: 502317 Rat
- Omim: 104775 Human
- SwissProt: P51693 Human
- SwissProt: Q03157 Mouse
- Unigene: 74565 Human
- Unigene: 2381 Mouse
別名
- AMYLOID BETA A4 PRECURSOR-LIKE PROTEIN 1 antibody
- AMYLOID PRECURSOR-LIKE PROTEIN antibody
- Amyloid-like protein 1 precursor antibody
- APLP 1 antibody
- APLP antibody
- APLP-1 antibody
- Aplp1 antibody
- APLP1_HUMAN antibody
- C30 antibody
see all

Western blot - Anti-APLP1 antibody [EPR25737-10] (ab291070)

All lanes : Anti-APLP1 antibody [EPR25737-10] (ab291070) at 1/1000 dilution

Lane 1 : Human cerebellum tissue lysate
Lane 2 : Human liver tissue lysate
Lane 3 : Human small intestine tissue lysate
Lane 4 : Human colon cancer tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 72 kDa
Observed band size: 97 kDa why is the actual band size different from the predicted?

Exposure time: 26 seconds

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Samples are non-boiled as boiling may cause protein aggregates.
The expression profile/molecular weight observed is consistent with that described in the literature (PMID: 7494461; PMID: 18430897).
Low expression: Liver, small intestine, colon cancer (PMID:18430897).
Western blot - Anti-APLP1 antibody [EPR25737-10] (ab291070)

All lanes : Anti-APLP1 antibody [EPR25737-10] (ab291070) at 1/1000 dilution

Lanes 1 & 7 : Mouse brain tissue lysate
Lane 2 : Mouse cerebral cortex tissue lysate
Lane 3 : Mouse liver tissue lysate
Lane 4 : Rat brain tissue lysate
Lane 5 : Rat cerebral cortex tissue lysate
Lane 6 : Rat liver tissue lysate
Lane 8 : Mouse brain tissue lysate treated with PNGase F

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 72 kDa
Observed band size: 72, 97 kDa why is the actual band size different from the predicted?

Exposure time: 26 seconds

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Samples are non-boiled as boiling may cause protein aggregates.
The expression profile/molecular weight observed is consistent with that described in the literature (PMID: 7494461; PMID: 18430897).
Low expression: Liver (PMID: 18430897 ).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APLP1 antibody [EPR25737-10] (ab291070)

Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labelling APLP1 with ab291070 at 1/5000, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human cerebrum (PMID: 9444352). The section was incubated with ab291070 for 10 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: PBS was used instead of primary antibody followed by ready to use secondary antibody LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins was used.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APLP1 antibody [EPR25737-10] (ab291070)

Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labelling APLP1 with ab291070 at 1/5000, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse cerebrum. The section was incubated with ab291070 for 10 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: PBS was used instead of primary antibody followed by ready to use secondary antibody LeicaDS9800 (Bond™ Polymer Refine Detection).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APLP1 antibody [EPR25737-10] (ab291070)

Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labelling APLP1 with ab291070 at 1/5000, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on rat cerebrum. The section was incubated with ab291070 for 10 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: PBS was used instead of primary antibody followed by ready to use secondary antibody LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins was used.
Flow Cytometry - Anti-APLP1 antibody [EPR25737-10] (ab291070)

Flow cytometric analysis of mouse primary neuron cells labelling APLP1 with Ab291070 at 1/50 dilution compared with a rabbit monoclonal IgG (ab172730) / Left isotype control. Goat Anti-Rabbit IgG (Alexa Fluor^® 488, ab150081) was used as the secondary antibody.

Gated on viable cells.
Immunoprecipitation - Anti-APLP1 antibody [EPR25737-10] (ab291070)

APLP1 was immunoprecipitated from 0.35 mg SH-SY5Y (human neuroblastoma epithelial cell), whole cell lysate with ab291070 at 1/30 dilution. Western blot was performed on the immunoprecipitate using ab291070 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 (Input): SH-SY5Y (human neuroblastoma epithelial cell), whole cell lysate 10 μg
Lane 2 (+): SH-SY5Y whole cell lysate

Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab291070 in SH-SY5Y whole cell lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 75 seconds
Immunoprecipitation - Anti-APLP1 antibody [EPR25737-10] (ab291070)

APLP1 was immunoprecipitated from 0.35 mg mouse brain tissue lysate 10 μg with ab291070 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab291070 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 (Input): mouse brain tissue lysate 10 μg

Lane 2 (+): Mouse brain tissue lysate

Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab291070 in Mouse brain tissue lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 7.5 seconds

The bands observed below 97kDa may be the degraded target protein because of long term incubation.
Anti-APLP1 antibody [EPR25737-10] (ab291070)

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

SDS download

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Datasheet download

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ab291070 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab291070 は論文での使用が確認できていません。

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Key features and details

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製品の概要

製品名

製品の詳細

由来種

アプリケーション

種交差性

免疫原

ポジティブ･コントロール

特記事項

製品の特性

製品の状態

保存方法

バッファー

精製度

ポリ/モノ

クローン名

アイソタイプ

研究分野

関連製品

Alternative Versions

Compatible Secondaries

Isotype control

アプリケーション

The Abpromise guarantee

ターゲット情報

機能

組織特異性

配列類似性

ドメイン

翻訳後修飾

細胞内局在

参照データベース

別名

画像

プロトコール

データシートおよび資料

SDS download

Datasheet download

Certificate of Compliance

参考文献 (0)

レビューと Q&A