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AB238092

Anti-APG5L/ATG5 抗体 [ATG5/2101]

Anti-APG5L/ATG5 antibody [ATG5/2101]

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(4 Publications)

Mouse Monoclonal APG5L/ATG5 antibody. Suitable for WB, Protein Array, IHC-P and reacts with Human, Recombinant full length protein - Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ATG5 aa 1-150.

別名を表示する

APG5L, ASP, ATG5, Autophagy protein 5, APG5-like, Apoptosis-specific protein

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APG5L/ATG5 antibody [ATG5/2101] (AB238092)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APG5L/ATG5 antibody [ATG5/2101] (AB238092)

Formalin-fixed, paraffin-embedded human brain tissue stained for APG5L/ATG5 using ab238092 at 2 μg/ml in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APG5L/ATG5 antibody [ATG5/2101] (AB238092)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APG5L/ATG5 antibody [ATG5/2101] (AB238092)

Formalin-fixed, paraffin-embedded human endometrium tissue stained for APG5L/ATG5 using ab238092 at 2 μg/ml in immunohistochemical analysis.

Western blot - Anti-APG5L/ATG5 antibody [ATG5/2101] (AB238092)
  • WB

Supplier Data

Western blot - Anti-APG5L/ATG5 antibody [ATG5/2101] (AB238092)

All lanes:

Western blot - Anti-APG5L/ATG5 antibody [ATG5/2101] (ab238092) at 2 µg/mL

Lane 1:

THP-1 (human monocytic leukemia cell line) cell lysate

Lane 2:

Human brain tissue lysate

Predicted band size: 32 kDa

false

Protein Array - Anti-APG5L/ATG5 antibody [ATG5/2101] (AB238092)
  • Protein Array

Supplier Data

Protein Array - Anti-APG5L/ATG5 antibody [ATG5/2101] (AB238092)

Analysis of Protein Array containing more than 19,000 full-length human proteins using ab238092.

Z- and S- Score : The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

ATG5/2101

アイソタイプ

IgG1

軽鎖のタイプ

kappa

キャリアフリー

No

交差種

Human

アプリケーション

WB, Protein Array, IHC-P

applications

免疫原

Recombinant Fragment Protein within Human ATG5 aa 1-150. The exact immunogen used to generate this antibody is proprietary information.

Q9H1Y0

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ProteinArray" : {"fullname" : "Protein Array", "shortname":"Protein Array"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>", "ProteinArray-species-checked": "guaranteed", "ProteinArray-species-dilution-info": "", "ProteinArray-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1 - 1/2", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Recombinant full length protein - Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ProteinArray-species-checked": "testedAndGuaranteed", "ProteinArray-species-dilution-info": "", "ProteinArray-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A/G
精製に関する特記事項
Purified from bioreactor concentrate.
バッファー組成
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

APG5L commonly known as ATG5 or ATG5 protein plays a significant role in autophagy. This molecular component has a molecular weight of approximately 32 kDa. ATG5 is expressed in multiple tissue types with a higher expression profile in immune-related cells. The protein is essential for the formation of the autophagosome as it conjugates with ATG12 to form a complex that is critical for autophagosome membrane elongation.
Biological function summary

ATG5 plays an important part in the cellular process of autophagy affecting the degradation and recycling of cellular components. It is a part of a complex with ATG12 and ATG16L1 which facilitates the membrane building of the autophagosome. This protein is important for maintaining cellular homeostasis allowing the removal of damaged proteins and organelles and is involved in responses to stress and starvation.

Pathways

ATG5 is an important component in the autophagy pathway and is involved in cellular maintenance processes. This pathway is vital for the regulation of cell growth and metabolism. ATG5 works closely with other autophagy-related proteins such as LC3. The ATG5-ATG12 complex helps in the lipidation of LC3 which is critical for autophagosome maturation connecting its function to the mTOR signaling pathway that regulates many cellular processes.

Disruption in ATG5 function is linked to neurodegenerative diseases and cancer. For instance dysregulation of autophagy due to ATG5 dysfunction may result in the progression of neurodegenerative disorders like Alzheimer's disease. Additionally in certain cancers altered autophagy can influence the survival of cancer cells. The interplay between ATG5 and other proteins like p53 and Bcl-2 is under investigation to understand its role in these diseases.

製品プロトコール

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ターゲットの情報

Involved in autophagic vesicle formation. Conjugation with ATG12, through a ubiquitin-like conjugating system involving ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes. Involved in mitochondrial quality control after oxidative damage, and in subsequent cellular longevity. Plays a critical role in multiple aspects of lymphocyte development and is essential for both B and T lymphocyte survival and proliferation. Required for optimal processing and presentation of antigens for MHC II. Involved in the maintenance of axon morphology and membrane structures, as well as in normal adipocyte differentiation. Promotes primary ciliogenesis through removal of OFD1 from centriolar satellites and degradation of IFT20 via the autophagic pathway. As part of the ATG8 conjugation system with ATG12 and ATG16L1, required for recruitment of LRRK2 to stressed lysosomes and induction of LRRK2 kinase activity in response to lysosomal stress (By similarity).. May play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. Plays a crucial role in IFN-gamma-induced autophagic cell death by interacting with FADD.. (Microbial infection) May act as a proviral factor. In association with ATG12, negatively regulates the innate antiviral immune response by impairing the type I IFN production pathway upon vesicular stomatitis virus (VSV) infection (PubMed : 17709747). Required for the translation of incoming hepatitis C virus (HCV) RNA and, thereby, for initiation of HCV replication, but not required once infection is established (PubMed : 19666601).
See full target information ATG5

文献 (4)

Recent publications for all applications. Explore the full list and refine your search

Molecular cancer therapeutics 24:1171-1185 PubMed40231599

2025

Azenosertib Is a Potent and Selective WEE1 Kinase Inhibitor with Broad Antitumor Activity Across a Range of Solid Tumors.

Applications

Unspecified application

Species

Unspecified reactive species

Jianhui Ma,Wen Liu,Jiali Li,Daehwan Kim,Sangyub Kim,Alexandra Levy,Zhen Cai,Kevin D Bunker,Alejandro Recio-Boiles,Jennifer M Segar,Shiraj Sen,Deborah B Doroshow,Danielle Jandial,Michael Rutgard,Olivier Harismendy,Stephan K Grant,Ahmed A Samatar,Kimberlee M Fischer,Mark R Lackner

Oxidative medicine and cellular longevity 2022:3504279 PubMed36062186

2022

Astrocytic IGF-1 and IGF-1R Orchestrate Mitophagy in Traumatic Brain Injury via Exosomal miR-let-7e.

Applications

Unspecified application

Species

Unspecified reactive species

Ren Dabin,Chen Wei,Shu Liang,Cao Ke,Wang Zhihan,Zheng Ping

Bioengineered 12:10187-10198 PubMed34860641

2021

Baicalin suppresses glaucoma pathogenesis by regulating the PI3K/AKT signaling in vitro and in vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Ningmin Zhao,Jieran Shi,Haohang Xu,Qing Luo,Qiaoyan Li,Mingzhou Liu

Oncology reports 45:665-679 PubMed33416155

2021

Enhancement of cisplatin sensitivity in human breast cancer MCF-7 cell line through BiP and 14-3-3ζ co-knockdown.

Applications

Unspecified application

Species

Unspecified reactive species

Tahereh Kashkoulinejad-Kouhi,Shahrokh Safarian,Blanca Arnaiz,Laura Saa
View all publications

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