Anti-Apelin 抗体 (ab59469)
Key features and details
- Rabbit polyclonal to Apelin
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Apelin antibody
Apelin 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Apelin -
由来種
Rabbit -
特異性
This antibody reacts specifically with 8,5 kDa of human APLN protein. -
アプリケーション
適用あり: IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Cow
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免疫原
Synthetic peptide corresponding to Human Apelin (C terminal).
Database link: Q9ULZ1 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
Constituent: Whole serum -
Concentration information loading... -
精製度
Whole antiserum -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab59469の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
1/500.
|
| 特記事項 |
|---|
|
IHC-P
1/500. |
ターゲット情報
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機能
Endogenous ligand for APJ, an alternative coreceptor with CD4 for HIV-1 infection. Inhibits HIV-1 entry in cells coexpressing CD4 and APJ. Apelin-36 has a greater inhibitory activity on HIV infection than other synthetic apelin derivatives. The oral intake in the colostrum and the milk could have a role in the modulation of the immune responses in neonates. May also have a role in the central control of body fluid homeostasis by influencing AVP release and drinking behavior. -
組織特異性
Expressed in the brain with highest levels in the frontal cortex, thalamus, hypothalamus and midbrain. Secreted by the mammary gland into the colostrum and the milk. -
配列類似性
Belongs to the apelin family. -
翻訳後修飾
Several active peptides may be produced by proteolytic processing of the peptide precursor. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 282143 Cow
- Entrez Gene: 8862 Human
- Entrez Gene: 30878 Mouse
- Entrez Gene: 58812 Rat
- Omim: 300297 Human
- SwissProt: Q9TUI9 Cow
- SwissProt: Q9ULZ1 Human
- SwissProt: Q9R0R4 Mouse
see all -
別名
- AGTRL1 ligand antibody
- APEL antibody
- APEL_HUMAN antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apelin antibody (ab59469)ab59469 (1:500) staining Apelin in human placenta using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of cytoplasmic/secreted regions of cells in the basal uterine glands.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
データシートおよび資料
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Datasheet download
参考文献 (19)
ab59469 は 19 報の論文で使用されています。
- Bommakanti K et al. Comparative Transcriptomic Analysis of Archival Human Vestibular Schwannoma Tissue from Patients with and without Tinnitus. J Clin Med 12:N/A (2023). PubMed: 37048724
- Zhang H et al. Apelin Rejuvenates Aged Human Mesenchymal Stem Cells by Regulating Autophagy and Improves Cardiac Protection After Infarction. Front Cell Dev Biol 9:628463 (2021). PubMed: 33738284
- Feng J et al. The Role of Apelin/APJ in a Mouse Model of Oxygen-induced Retinopathy. Invest Ophthalmol Vis Sci 61:47 (2020). PubMed: 32729912
- Xu C et al. ELABELA antagonizes intrarenal renin-angiotensin system to lower blood pressure and protects against renal injury. Am J Physiol Renal Physiol 318:F1122-F1135 (2020). PubMed: 32174138
- Mastrella G et al. Targeting APLN/APLNR Improves Antiangiogenic Efficiency and Blunts Proinvasive Side Effects of VEGFA/VEGFR2 Blockade in Glioblastoma. Cancer Res 79:2298-2313 (2019). PubMed: 30718358
