APC/Cy5.5® Anti-CD8 alpha 抗体 [53-6.7] (ab82005)
![Flow Cytometry - APC/Cy5.5® Anti-CD8 alpha antibody [53-6.7] (ab82005)](https://www.abcam.co.jp/ps/products/82/ab82005/Images/ab82005-272369-anti-cd8-alpha-antibody-53-67-allophycocyanin-cy55-flow-cytometry.jpg "Flow Cytometry - APC/Cy5.5® Anti-CD8 alpha antibody [53-6.7] (ab82005)")
Key features and details
- APC/Cy5.5® Rat monoclonal [53-6.7] to CD8 alpha
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: APC/Cy5.5®. Ex: 650nm, Em: 690nm
- Isotype: IgG2a
Related conjugates and formulations
製品の概要
-
製品名
APC/Cy5.5® Anti-CD8 alpha antibody [53-6.7]
CD8 alpha 一次抗体 製品一覧 -
製品の詳細
APC/Cy5.5® Rat monoclonal [53-6.7] to CD8 alpha -
由来種
Rat -
標識
APC/Cy5.5®. Ex: 650nm, Em: 690nm -
アプリケーション
適用あり: Flow Cytmore details -
種交差性
交差種: Mouse -
免疫原
Tissue, cells or virus corresponding to CD8 alpha. Mouse spleen cells or thymocyte membranes
-
ポジティブ･コントロール
- Mouse mesenteric lymph node cells.
-
特記事項
In vivo and in vitro treatment with the clone 53-6.7 monoclonal antibody effectively depletes CD8a+ cells. Clone 53-6.7 also blocks allogeneic help specific for class I MHC antigens and T cell responses to IL-2.
This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5, 268, 486 and related patents. Cy® and CyDye® are trademarks of Cytiva.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Store In the Dark. -
バッファー
pH: 7.1
Preservative: 0.09% Sodium azide
Constituents: 16% Sucrose, PBS
Also contains a stabilizing agent. -
Concentration information loading... -
精製度
Affinity purified -
一次抗体 備考
In vivo and in vitro treatment with the clone 53-6.7 monoclonal antibody effectively depletes CD8a+ cells. Clone 53-6.7 also blocks allogeneic help specific for class I MHC antigens and T cell responses to IL-2. -
ポリ/モノ
モノクローナル -
クローン名
53-6.7 -
アイソタイプ
IgG2a -
軽鎖の種類
kappa -
研究分野
関連製品
-
Alternative Versions
- Biotin Anti-CD8 alpha antibody [53-6.7] (ab25176)
- PE/Texas Red® Anti-CD8 alpha antibody [53-6.7] (ab25294)
- Anti-CD8 alpha antibody [53-6.7] (ab25478)
- PE Anti-CD8 alpha antibody [53-6.7] (ab25498)
- APC Anti-CD8 alpha antibody [53-6.7] (ab25499)
- FITC Anti-CD8 alpha antibody [53-6.7] (ab25676)
- PE Anti-CD8 alpha antibody [53-6.7] (ab272343)
- Cy5 ® Anti-CD8 alpha antibody [53-6.7] (ab39850)
- PE/Cy7® Anti-CD8 alpha antibody [53-6.7] (ab39853)
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab82005の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt |
Use 0.2µg for 106 cells.
|
| 特記事項 |
|---|
|
Flow Cyt
Use 0.2µg for 106 cells. |
ターゲット情報
-
機能
Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains. -
関連疾患
Defects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections. -
配列類似性
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
翻訳後修飾
All of the five most carboxyl-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not. -
細胞内局在
Secreted and Cell membrane. - Information by UniProt
-
参照データベース
- Entrez Gene: 12525 Mouse
- SwissProt: P01731 Mouse
- Unigene: 1858 Mouse
-
別名
- alpha polypeptide (p32) antibody
- CD_antigen=CD8a antibody
- CD8 antibody
see all
画像
-
Flow Cytometry - APC/Cy5.5® Anti-CD8 alpha antibody [53-6.7] (ab82005)Flow cytometry analysis of BALB/c mouse splenocytes labelling CD8 alpha with ab82005 at 0.1 µg/106cells and CD19 with rat anti-mouse CD19-FITC.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (4)
ab82005 は 4 報の論文で使用されています。
- Huynh N et al. Glycogen branching enzyme controls cellular iron homeostasis via Iron Regulatory Protein 1 and mitoNEET. Nat Commun 10:5463 (2019). PubMed: 31784520
- Takahashi K et al. CD4 and CD8 regulate interleukin 2 responses of T cells. Proc Natl Acad Sci U S A 89:5557-61 (1992). Depletion ; Mouse . PubMed: 1608966
- Ledbetter JA et al. T cell subsets defined by expression of Lyt-1,2,3 and Thy-1 antigens. Two-parameter immunofluorescence and cytotoxicity analysis with monoclonal antibodies modifies current views. J Exp Med 152:280-95 (1980). Flow Cyt ; Mouse . PubMed: 6156984
- Ledbetter JA & Herzenberg LA Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens. Immunol Rev 47:63-90 (1979). Flow Cyt, IP ; Mouse . PubMed: 398327