Anti-Apc1 (phospho S377) 抗体 (ab12206)
Key features and details
- Rabbit polyclonal to Apc1 (phospho S377)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Apc1 (phospho S377) antibody
Apc1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Apc1 (phospho S377) -
由来種
Rabbit -
特異性
This antibody is specific for phosphorylated human APC1 protein at the pS377 residue. A BLAST analysis was used to suggest reactivity with this protein from human, dog, rat, and bovine based on 100% homology for the immunogen sequence. Cross reactivity with APC1 protein from mouse is expected, as this sequence show 90% homologous between human and mouse. Cross reactivity with APC1 homologues from other sources has not been determined. Minimal reactivity is expected with the non-phosphorylated form of the protein.
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アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Cow, Dog
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 0.424% Tripotassium orthophosphate, 0.88% Sodium chloride -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
This product is an affinity purified antibody produced by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab12206の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/200 - 1/1000. Detects a band of approximately 215 kDa (predicted molecular weight: 215 kDa).
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| IHC-P |
Use at an assay dependent concentration.
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| 特記事項 |
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WB
1/200 - 1/1000. Detects a band of approximately 215 kDa (predicted molecular weight: 215 kDa). |
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IHC-P
Use at an assay dependent concentration. |
ターゲット情報
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機能
Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. -
パスウェイ
Protein modification; protein ubiquitination. -
配列類似性
Belongs to the APC1 family.
Contains 4 PC repeats. -
翻訳後修飾
Phosphorylated. Phosphorylation on Ser-355 occurs specifically during mitosis. - Information by UniProt
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参照データベース
- Entrez Gene: 535565 Cow
- Entrez Gene: 475750 Dog
- Entrez Gene: 64682 Human
- Entrez Gene: 17222 Mouse
- Omim: 608473 Human
- SwissProt: E1BAB6 Cow
- SwissProt: E2RLM2 Dog
- SwissProt: Q9H1A4 Human
see all -
別名
- ANAPC 1 antibody
- Anapc1 antibody
- Anaphase promoting complex subunit 1 antibody
see all
画像
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Western blot - Anti-Apc1 (phospho S377) antibody (ab12206)All lanes : Anti-Apc1 (phospho S377) antibody (ab12206) at 1/1000 dilution
Lane 1 : HeLa whole cell lysate from asynchoronous cells
Lane 2 : HeLa whole cell lysate from cells treated with nocodazole
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat-anti-Rabbit IgG [H&L] MX at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 215 kDa
Observed band size: 215 kDaThe blot was probed with ab12206 overnight at 4°C
While some phosphorylated APC1 is present in untreated cells, the amount of phosphorylated protein is increased arrested in mitosis (by treatment with with nocodazole).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apc1 (phospho S377) antibody (ab12206)ab12206 at 5.0 µg/ml staining pancreatic cancinoma cells at 60X. This image shows moderate positive cytoplasmic and occasional nuclear staining. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab12206 は論文での使用が確認できていません。