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AB320195

APC Anti-SF3B1 抗体 [EPR11986]

APC Anti-SF3B1 antibody [EPR11986]

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Rabbit Recombinant Monoclonal SF3B1 antibody - conjugated to APC.

別名を表示する

SAP155, SF3B1, Splicing factor 3B subunit 1, Pre-mRNA-splicing factor SF3b 155 kDa subunit, Spliceosome-associated protein 155, SF3b155, SAP 155

関連する標識済み抗体及び組成の異なる製品 (9)

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR11986

アイソタイプ

IgG

標識

APC

励起波長/蛍光波長

Ex: 650nm, Em: 660nm

キャリアフリー

No

アプリケーション

Antibody Labelling, Target Binding Affinity

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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製品の詳細

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

How are conjugated primary antibodies validated?
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
+4°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle|Store in the dark
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

SF3B1 also known as Splicing Factor 3B subunit 1 plays an important role in the mRNA splicing process. This protein participates in the splicing machinery as part of the spliceosome contributing to the removal of introns from pre-mRNA. SF3B1 has a molecular weight of approximately 160 kDa. It is ubiquitously expressed yet shows a high level of activity in tissues with rapid cell division such as the bone marrow and lymphatic tissue reflecting its central involvement in gene expression regulation.
Biological function summary

SF3B1 is an essential component of the spliceosomal complex specifically the U2 snRNP component. It helps establish the branchpoint recognition complex ensuring accurate RNA splicing necessary for producing functional mRNA molecules. This process is important for the expression of diverse protein-coding genes affecting multiple aspects of cellular function and identity. The protein interacts with other spliceosomal proteins like SF3A and U2AF to guide the precise removal of non-coding sequences enabling proper translation into proteins.

Pathways

SF3B1 is involved in the mRNA processing pathway and influences the cell cycle pathway. During mRNA processing SF3B1 aids in assembling the spliceosome facilitating the excision of introns. The protein interacts with components like SF3B2 in coordinating splicing with other RNA processing events. In the cell cycle pathway SF3B1 indirectly affects gene expression and stability influencing cell cycle progression and serving as a regulatory node by modulating the splicing of key regulators active during cell division.

Several studies associate SF3B1 mutations with certain types of cancer including myelodysplastic syndrome and chronic lymphocytic leukemia. These mutations often result in aberrant splicing events leading to altered gene expression profiles that can promote tumorigenesis. Within these disease contexts SF3B1 abnormalities can influence interactions with related proteins like SRSF2 contributing to misregulated splicing patterns of genes that drive cancerous transformations and affect patient prognosis.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Component of the 17S U2 SnRNP complex of the spliceosome, a large ribonucleoprotein complex that removes introns from transcribed pre-mRNAs (PubMed : 12234937, PubMed : 27720643, PubMed : 32494006, PubMed : 34822310). The 17S U2 SnRNP complex (1) directly participates in early spliceosome assembly and (2) mediates recognition of the intron branch site during pre-mRNA splicing by promoting the selection of the pre-mRNA branch-site adenosine, the nucleophile for the first step of splicing (PubMed : 32494006, PubMed : 34822310). Within the 17S U2 SnRNP complex, SF3B1 is part of the SF3B subcomplex, which is required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence in pre-mRNA (PubMed : 12234937). Sequence independent binding of SF3A and SF3B subcomplexes upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA (PubMed : 12234937). May also be involved in the assembly of the 'E' complex (PubMed : 10882114). Also acts as a component of the minor spliceosome, which is involved in the splicing of U12-type introns in pre-mRNAs (PubMed : 15146077, PubMed : 33509932). Together with other U2 snRNP complex components may also play a role in the selective processing of microRNAs (miRNAs) from the long primary miRNA transcript, pri-miR-17-92 (By similarity).
See full target information SF3B1
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組合せ製品

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AB233565

Anti-SF3B4 antibody [3A1]

Immunocytochemistry/ Immunofluorescence - Anti-SF3B4 antibody [3A1] (AB233565)

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Primary Antibodies

AB220163

Anti-YTHDF2 antibody [EPR20318]

RabMAb|Recombinant|KO Validated

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