AB237383

APC Anti-NCAM1 抗体 [EPR21827]

APC Anti-NCAM1 antibody [EPR21827]

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal NCAM1 antibody - conjugated to APC. Suitable for Flow Cyt, ICC/IF, IHC-P and reacts with Mouse, Rat samples. Cited in 1 publication.

別名を表示する

CD56, Ncam, Ncam1, Neural cell adhesion molecule 1, N-CAM-1, NCAM-1

2 Images

Immunocytochemistry/ Immunofluorescence - APC Anti-NCAM1 antibody [EPR21827] (AB237383)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - APC Anti-NCAM1 antibody [EPR21827] (AB237383)

Immunofluorescent analysis of 100% methanol-fixed Neuro2a (Mouse neuroblastoma cell line) cells labeling NCAM1 with ab237383 at 1/100 dilution (5 μg/ml), followed by ab195887 anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 488) (green). The nuclear counterstain is DAPI (blue).

Negative control : L-929 cells.

Flow Cytometry - APC Anti-NCAM1 antibody [EPR21827] (AB237383)

Flow Cyt

Lab

Flow Cytometry - APC Anti-NCAM1 antibody [EPR21827] (AB237383)

Flow cytometric analysis of L-929 (Mouse connective tissue fibroblast cell line, Left) / Neuro2a (Mouse neuroblastoma cell line, Right) labeling NCAM1 with ab237383 at 1/500 dilution (red) compared with ab232814 Rabbit IgG, monoclonal [EPR25A] - Isotype Control - Allophycocyanin (Black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue).

Negative control : L-929 cells.

Gated on viable cells.

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR21827

アイソタイプ

IgG

標識

APC

励起波長/蛍光波長

Ex: 650nm, Em: 660nm

キャリアフリー

交差種

Mouse, Rat

アプリケーション

Flow Cyt, ICC/IF, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/500", "FlowCyt-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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製品の詳細

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

+4°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Do Not Freeze|Store in the dark

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

NCAM1 also known as Neural Cell Adhesion Molecule 1 or CD56 is an important cell surface glycoprotein that plays a role in cell-cell adhesion. It has a molecular mass of approximately 120-140 kDa. NCAM1 is widely expressed in various tissues including the nervous system skeletal muscle and certain hematopoietic cells. In flow cytometry and immunohistochemistry NCAM1's expression is extensively studied to track and analyze cell populations using techniques like NCAM1 ELISA for quantification.

Biological function summary

NCAM1 influences neuronal growth survival and synaptic plasticity. This protein is part of the immunoglobulin superfamily and contributes to dynamic interactions during development. NCAM1 exists on cell membranes assisting in the formation and maintenance of tissue structures. In particular cell adhesion molecule CAL53 interacts with NCAM1 highlighting its involvement in synaptic connections. Additionally NCAM1 impacts cellular migration and differentiation particularly in myogenic processes involving MY31's pathway.

Pathways

The NCAM1 protein participates in the MAPK signaling pathway and the PI3K/AKT pathway both essential in cellular growth and survival. NCAM1 acts alongside ERIC1 aiding in the transduction of signals that regulate cytoskeletal rearrangement. These pathways influence cellular communication and responses to external stimuli further enhancing tissue development and regeneration mechanisms.

NCAM1 is associated with neurodegenerative conditions like Alzheimer's disease and certain types of cancer including neuroblastoma. Changes in NCAM1 expression or function can disrupt normal cell adhesion and signaling leading to pathological states. The protein's interaction with cell surface markers such as CD56 mouse marker helps in the diagnosis and therapy of these disorders providing potential therapeutic targets or biomarkers for clinical applications.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc.

See full target information Ncam1

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Mediators of inflammation 2023:8215567 PubMed37035756

2023

T Cell Subsets and the Expression of Related MicroRNAs in Patients with Recurrent Early Pregnancy Loss.

Applications

Unspecified application

Species

Unspecified reactive species

Ya-Ni Yan,Jian Zhang,Na Yang,Chaochao Chen,Weiwei Li

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

APC Anti-NCAM1 抗体 [EPR21827]

APC Anti-NCAM1 antibody [EPR21827]

Immunocytochemistry/ Immunofluorescence - APC Anti-NCAM1 antibody [EPR21827] (AB237383)

Flow Cytometry - APC Anti-NCAM1 antibody [EPR21827] (AB237383)

関連する標識済み抗体及び組成の異なる製品 (7)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

標識

励起波長/蛍光波長

キャリアフリー

交差種

アプリケーション

免疫原

組合せ製品

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (1)

Abcam product promise