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AB93539

APC Anti-Integrin alpha 4+beta 7 抗体 [DATK32 (DATK-32)]

APC Anti-Integrin alpha 4+beta 7 antibody [DATK32 (DATK-32)]

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(3 Publications)

Anti-Integrin alpha 4+beta 7 antibody [DATK32 (DATK-32)] conjugated to APC - suitable for flow cytometry, and reacts with mouse samples. Clone [DATK32 (DATK-32)] is a rat monoclonal antibody cited in 18 publications.

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別名を表示する

CD49d, Integrin alpha-4, CD49 antigen-like family member D, Integrin alpha-IV, Lymphocyte Peyer patch adhesion molecules subunit alpha, VLA-4 subunit alpha, LPAM subunit alpha, Itga4, Integrin beta-7, Integrin beta-P, M290 IEL antigen, Itgb7

1 Images
Flow Cytometry - APC Anti-Integrin alpha 4+beta 7 antibody [DATK32 (DATK-32)] (AB93539)
  • Flow Cyt

Supplier Data

Flow Cytometry - APC Anti-Integrin alpha 4+beta 7 antibody [DATK32 (DATK-32)] (AB93539)

C57Bl/6 bone marrow cells stained with FITC-conjugated anti-mouse CD45 (B220) and 0.25μg ab93539 (right) or 0.25μg APC-conjugated rat IgG2a isotype control (left).

Key facts

宿主種

Rat

クローン性

Monoclonal

クローン番号

DATK32 (DATK-32)

アイソタイプ

IgG2a

軽鎖のタイプ

kappa

標識

APC

励起波長/蛍光波長

Ex: 650nm, Em: 660nm

キャリアフリー

No

交差種

Mouse

アプリケーション

Flow Cyt

applications

免疫原

Cell preparation containing Itga4 protein. The exact immunogen used to generate this antibody is proprietary information.

Q00651

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代替製品

Primary Antibodies

AB95788

PE Anti-Integrin alpha 4+beta 7 antibody [DATK32]

Flow Cytometry - PE Anti-Integrin alpha 4+beta 7 antibody [DATK32] (AB95788)

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  • 0

組合せ製品

Primary Antibodies

AB24982

Cy5 ® Anti-CD45.1 antibody [A20]

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  • 0
Primary Antibodies

AB237365

Alexa Fluor® 647 Anti-CD8 alpha antibody [EPR21769]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CD8 alpha antibody [EPR21769] (AB237365)

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Primary Antibodies

AB252149

Alexa Fluor® 488 Anti-CD4 antibody [EPR20122]

RabMAb|Recombinant

Flow Cytometry - Alexa Fluor® 488 Anti-CD4 antibody [EPR20122] (AB252149)

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  • 0
Primary Antibodies

AB307134

Alexa Fluor® 488 Anti-CD3 antibody [SP162]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-CD3 antibody [SP162] (AB307134)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>" } } }
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製品の詳細

Binding of the DATK32 monoclonal antibody has been shown to induce aggregation of the CD8+ T cell lymphoma TK1, and block Integrin alpha 4 beta 7 mediated cell adhesion.
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Immunogen
バッファー組成
pH: 7.2 Preservative: 0.09% Sodium azide Constituents: 0.88% Sodium chloride, 0.12% Sodium dihydrogen phosphate, 0.1% Gelatin
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
+4°C
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Integrin alpha 4 beta 7 also known as alpha 4 beta 7 integrin A4B7 integrin and beta-7 is a cell surface integrin receptor involved in mediating cell adhesion. This target plays a mechanical role in binding to the mucosal addressin cell adhesion molecule-1 (MAdCAM-1) primarily on high endothelial venules in the gut-associated lymphoid tissue. It forms a heterodimer composed of the alpha 4 and beta 7 subunits with an estimated mass of about 150–180 kDa. Integrin alpha 4 beta 7 is expressed on the surface of leukocytes including T cells B cells and other immune cells facilitating their tissue-specific homing.
Biological function summary

Integrin alpha 4 beta 7 serves an important role in the migration and homing of lymphocytes to gut-associated lymphoid tissues ensuring immune surveillance. It is a part of the integrin family complex interacting with MAdCAM-1 to guide lymphocytes across the endothelium into the intestinal lamina propria. This process is essential for maintaining the immune cell balance within the gastrointestinal tract where the immune system encounters numerous antigens.

Pathways

Integrin alpha 4 beta 7 fits into immune cell trafficking and adhesion pathways. It works closely with proteins like MAdCAM-1 and interacts with the Janus kinase-signal transducer and activator of transcription (JAK-STAT) signaling pathway. These interactions reinforce the pivotal role of integrin in the precise targeting and localization of immune cells which is necessary for effective immune responses and homeostasis maintenance within specific tissue environments.

Integrin alpha 4 beta 7 connects with conditions such as inflammatory bowel diseases including Crohn's disease and ulcerative colitis. Its interaction with MAdCAM-1 contributes to the recruitment of inflammatory cells to the gut exacerbating inflammation in these disorders. Moreover integrin alpha 4 beta 7 has been implicated in HIV infection where it serves as a marker for lymphocytes targeted by the virus highlighting its potential as a therapeutic target for modulating immune system activity in disease contexts.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Integrins alpha-4/beta-1 (VLA-4 or LPAM-2) and alpha-4/beta-7 (LPAM-1) are receptors for fibronectin. They recognize one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. They are also receptors for VCAM1. Integrin alpha-4/beta-1 recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-4/beta-7 is also a receptor for MADCAM1. It recognizes the sequence L-D-T in MADCAM1. On activated endothelial cells integrin VLA-4 triggers homotypic aggregation for most VLA-4-positive leukocyte cell lines. It may also participate in cytolytic T-cell interactions with target cells. ITGA4 : ITGB1 binds to fractalkine (CX3CL1) and may act as its coreceptor in CX3CR1-dependent fractalkine signaling. ITGA4 : ITGB1 binds to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1. Integrin ITGA4 : ITGB1 represses PRKCA-mediated L-type voltage-gated channel Ca(2+) influx and ROCK-mediated calcium sensitivity in vascular smooth muscle cells via its interaction with SVEP1, thereby inhibiting vasocontraction (PubMed : 35802072).
See full target information Itga4

追加のターゲット

Itgb7
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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Journal of immunology (Baltimore, Md. : 1950) 153:3847-61 PubMed7523506

1994

Distinct but overlapping epitopes are involved in alpha 4 beta 7-mediated adhesion to vascular cell adhesion molecule-1, mucosal addressin-1, fibronectin, and lymphocyte aggregation.

Applications

Flow Cyt

Species

Unspecified reactive species

D P Andrew,C Berlin,S Honda,T Yoshino,A Hamann,B Holzmann,P J Kilshaw,E C Butcher

Journal of immunology (Baltimore, Md. : 1950) 152:3282-93 PubMed7511642

1994

Role of alpha 4-integrins in lymphocyte homing to mucosal tissues in vivo.

Applications

Flow Cyt

Species

Unspecified reactive species

A Hamann,D P Andrew,D Jablonski-Westrich,B Holzmann,E C Butcher

Cell 74:185-95 PubMed7687523

1993

Alpha 4 beta 7 integrin mediates lymphocyte binding to the mucosal vascular addressin MAdCAM-1.

Applications

Flow Cyt

Species

Unspecified reactive species

C Berlin,E L Berg,M J Briskin,D P Andrew,P J Kilshaw,B Holzmann,I L Weissman,A Hamann,E C Butcher
View all publications
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