APC Anti-Integrin alpha 2 抗体 [DX5] (ab41460)
![Flow Cytometry - APC Anti-Integrin alpha 2 antibody [DX5] (ab41460)](https://www.abcam.co.jp/ps/products/41/ab41460/Images/ab41460-1-anti-integrin-alpha-2-antibody-dx5-apc-flow-cytometry-splenocytes-mouse.jpg "Flow Cytometry - APC Anti-Integrin alpha 2 antibody [DX5] (ab41460)")
Key features and details
- APC Rat monoclonal [DX5] to Integrin alpha 2
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: APC. Ex: 645nm, Em: 660nm
- Isotype: IgM
製品の概要
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製品名
APC Anti-Integrin alpha 2 antibody [DX5]
Integrin alpha 2 一次抗体 製品一覧 -
製品の詳細
APC Rat monoclonal [DX5] to Integrin alpha 2 -
由来種
Rat -
標識
APC. Ex: 645nm, Em: 660nm -
アプリケーション
適用あり: Flow Cytmore details -
種交差性
交差種: Mouse -
免疫原
Tissue, cells or virus corresponding to Mouse Integrin alpha 2. NK cells isolated from C57BL/6 mice
Database link: Q62469 -
ポジティブ･コントロール
- Flow Cyt: C57BL/6 mouse splenocytes.
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特記事項
This monoclonal antibody, clone DX5, reacts with CD49b, also known as very late antigen-2 (VLA-2) and Integrin alpha 2. The antibody stains the majority of NK cells and a small subpopulation of T cells in all mouse strains tested (e.g., A/J, AKR, BALB/c, C3H/HeJ, C57BL/6, C57BL/10, C57BR, C58, CBA/Ca, CBA/J, DBA/1, DBA/2, SJL, SWR). Three-color flow cytometry analysis has revealed that most cells that express NK-1.1 (NKR-P1C) also express CD49b. However, small subsets of DX5+NK-1.1- and DX5-NK-1.1+ cells can be found, especially among the CD3+ population of T lymphocytes.2 NK cells cultured in the presence of IL-2 progressively lose reactivity with DX5 as a consequence of cellular proliferation. Therefore, DX5 can be used to define functionally distinct subsets of murine NK cells. The monoclonal antibody DX5 has not been demonstrated to have activating or blocking activity.
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製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Store In the Dark. -
バッファー
pH: 7.3
Preservative: 0.09% Sodium azide
Constituents: PBS, 16% Sucrose
Also contains stabilising agent. -
Concentration information loading... -
精製度
Purified IgM -
一次抗体 備考
This monoclonal antibody, clone DX5, reacts with CD49b, also known as very late antigen-2 (VLA-2) and Integrin alpha 2. The antibody stains the majority of NK cells and a small subpopulation of T cells in all mouse strains tested (e.g., A/J, AKR, BALB/c, C3H/HeJ, C57BL/6, C57BL/10, C57BR, C58, CBA/Ca, CBA/J, DBA/1, DBA/2, SJL, SWR). Three-color flow cytometry analysis has revealed that most cells that express NK-1.1 (NKR-P1C) also express CD49b. However, small subsets of DX5+NK-1.1- and DX5-NK-1.1+ cells can be found, especially among the CD3+ population of T lymphocytes.2 NK cells cultured in the presence of IL-2 progressively lose reactivity with DX5 as a consequence of cellular proliferation. Therefore, DX5 can be used to define functionally distinct subsets of murine NK cells. The monoclonal antibody DX5 has not been demonstrated to have activating or blocking activity. -
ポリ/モノ
モノクローナル -
クローン名
DX5 -
ミエローマ
Sp2/0 -
アイソタイプ
IgM -
軽鎖の種類
kappa -
研究分野
関連製品
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Alternative Versions
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab41460の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
|
Flow Cyt
Use at an assay dependent concentration. |
ターゲット情報
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機能
Integrin alpha-2/beta-1 is a receptor for laminin, collagen, collagen C-propeptides, fibronectin and E-cadherin. It recognizes the proline-hydroxylated sequence G-F-P-G-E-R in collagen. It is responsible for adhesion of platelets and other cells to collagens, modulation of collagen and collagenase gene expression, force generation and organization of newly synthesized extracellular matrix. -
配列類似性
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats.
Contains 1 VWFA domain. -
ドメイン
The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 16398 Mouse
- SwissProt: Q62469 Mouse
- Unigene: 5007 Mouse
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別名
- BR antibody
- CD 49b antibody
- CD49 antigen like family member B antibody
see all
画像
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Flow Cytometry - APC Anti-Integrin alpha 2 antibody [DX5] (ab41460)
Flow Cytometry analysis of C57BL/6 mouse splenocytes stained for Integrin alpha 2 using ab41460 (0.1 µg/106 cells) and a Mouse Anti-Mouse NK1.1-PE.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab41460 は 3 報の論文で使用されています。
- Obokata H et al. Reproducible subcutaneous transplantation of cell sheets into recipient mice. Nat Protoc 6:1053-9 (2011). PubMed: 21720318
- Arase H et al. Cutting edge: the mouse NK cell-associated antigen recognized by DX5 monoclonal antibody is CD49b (alpha 2 integrin, very late antigen-2). J Immunol 167:1141-4 (2001). PubMed: 11466327
- Moore TA et al. Inhibition of gamma delta T cell development and early thymocyte maturation in IL-7 -/- mice. J Immunol 157:2366-73 (1996). PubMed: 8805634