AB224984

APC Anti-Cytokeratin 5 抗体 [EP1601Y]

APC Anti-Cytokeratin 5 antibody [EP1601Y]

RabMAb
Recombinant
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(4 Publications)

Rabbit Recombinant Monoclonal Cytokeratin 5 antibody - conjugated to APC. Suitable for Flow Cyt (Intra), ICC/IF, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 4 publications.

別名を表示する

58 kDa cytokeratin, Cytokeratin-5, Keratin-5, Type-II keratin Kb5, CK-5, K5, KRT5, CK5

1 Images

Flow Cytometry (Intracellular) - APC Anti-Cytokeratin 5 antibody [EP1601Y] (AB224984)

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - APC Anti-Cytokeratin 5 antibody [EP1601Y] (AB224984)

Overlay histogram showing A431 cells stained with ab224984 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab224984, 1/5000 dilution) for 30 min at 22°C.

Isotype control antibody (black line) was Rabbit IgG (monoclonal) Allophycocyanin used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5,000 events were collected using a 40 mW Red laser (640nm) and 670/14 bandpass filter.

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EP1601Y

アイソタイプ

IgG

標識

APC

励起波長/蛍光波長

Ex: 650nm, Em: 660nm

キャリアフリー

交差種

Human, Human, Mouse, Rat

アプリケーション

Flow Cyt (Intra), IHC-P, ICC/IF

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/5000", "FlowCytIntra-species-notes": "The cellular localisation of this product has been verified in ICC/IF.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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製品の詳細

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Do Not Freeze|Store in the dark

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Required for the formation of keratin intermediate filaments in the basal epidermis and maintenance of the skin barrier in response to mechanical stress (By similarity). Regulates the recruitment of Langerhans cells to the epidermis, potentially by modulation of the abundance of macrophage chemotactic cytokines, macrophage inflammatory cytokines and CTNND1 localization in keratinocytes (By similarity).

See full target information KRT5

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文献 (4)

Recent publications for all applications. Explore the full list and refine your search

Nature genetics 57:2177-2191 PubMed40858905

2025

ERG-driven prostate cancer initiation is cell-context dependent and requires KMT2A and DOT1L.

Applications

Unspecified application

Species

Unspecified reactive species

Weiran Feng,Erik Ladewig,Matthew Lange,Nazifa Salsabeel,Huiyong Zhao,Young Sun Lee,Anuradha Gopalan,Hanzhi Luo,Wenfei Kang,Ning Fan,Eric Rosiek,Elisa de Stanchina,Yu Chen,Brett S Carver,Christina S Leslie,Charles L Sawyers

Nature 646:404-412 PubMed40836097

2025

SLC45A4 is a pain gene encoding a neuronal polyamine transporter.

Applications

Unspecified application

Species

Unspecified reactive species

Steven J Middleton,Sigurbjörn Markússon,Mikael Åkerlund,Justin C Deme,Mandy Tseng,Wenqianglong Li,Sana R Zuberi,Gabriel Kuteyi,Peter Sarkies,Georgios Baskozos,Jimena Perez-Sanchez,Adham Farah,Harry L Hébert,Sylvanus Toikumo,Zhanru Yu,Susan Maxwell,Yin Y Dong,Benedikt M Kessler,Henry R Kranzler,John E Linley,Blair H Smith,Susan M Lea,Joanne L Parker,Valeriya Lyssenko,Simon Newstead,David L Bennett

Military Medical Research 9:13 PubMed35351192

2022

Small molecules facilitate single factor-mediated sweat gland cell reprogramming.

Applications

Unspecified application

Species

Unspecified reactive species

Shuai-Fei Ji,Lai-Xian Zhou,Zhi-Feng Sun,Jiang-Bing Xiang,Shao-Yuan Cui,Yan Li,Hua-Ting Chen,Yi-Qiong Liu,Huan-Huan Gao,Xiao-Bing Fu,Xiao-Yan Sun

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 8:e2103079 PubMed34569165

2021

Sweat Gland Organoids Originating from Reprogrammed Epidermal Keratinocytes Functionally Recapitulated Damaged Skin.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoyan Sun,Jiangbing Xiang,Runkai Chen,Zhijun Geng,Lintao Wang,Yiqiong Liu,Shuaifei Ji,Huating Chen,Yan Li,Cuiping Zhang,Peng Liu,Tao Yue,Lei Dong,Xiaobing Fu

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

APC Anti-Cytokeratin 5 抗体 [EP1601Y]

APC Anti-Cytokeratin 5 antibody [EP1601Y]

Flow Cytometry (Intracellular) - APC Anti-Cytokeratin 5 antibody [EP1601Y] (AB224984)

関連する標識済み抗体及び組成の異なる製品 (7)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

標識

励起波長/蛍光波長

キャリアフリー

交差種

アプリケーション

免疫原

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

製品プロトコール

ターゲットの情報

文献 (4)

Abcam product promise