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AB210348

APC/Cy7® Anti-CD4 抗体 [RM4-5]

APC/Cy7® Anti-CD4 antibody [RM4-5]

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(4 Publications)

Anti-CD4 antibody [RM4-5] conjugated to APC/Cy7® - suitable for flow cytometry, and reacts with mouse samples. Clone [RM4-5] is a rat monoclonal antibody and one of the most commonly used clones for flow cytometry.

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別名を表示する

CD4, T-cell surface glycoprotein CD4, T-cell differentiation antigen L3T4, T-cell surface antigen T4/Leu-3, Cd4

1 Images
Flow Cytometry - APC/Cy7® Anti-CD4 antibody [RM4-5] (AB210348)
  • Flow Cyt

Supplier Data

Flow Cytometry - APC/Cy7® Anti-CD4 antibody [RM4-5] (AB210348)

Flow cytometric analysis of C57Bl/6 splenocytes labeling CD4 with 0.25 μg ab210348 (solid line), compared with 0.25 μg Allophycocyanin/Cy7® Rat IgG2a (dashed line).

関連する標識済み抗体及び組成の異なる製品 (6)

  • 660 APC

    APC Anti-CD4 antibody [RM4-5]

  • 519 FITC

    FITC Anti-CD4 antibody [RM4-5]

  • 578 PE

    PE Anti-CD4 antibody [RM4-5]

  • 778 PE/Cy7®

    PE/Cy7® Anti-CD4 antibody [RM4-5]

  • 704 redFluor™ 710

    redFluor™ 710 Anti-CD4 antibody [RM4-5]

  • 450 violetFluor™ 450

    violetFluor™ 450 Anti-CD4 antibody [RM4-5]

Key facts

宿主種

Rat

クローン性

Monoclonal

クローン番号

RM4-5

アイソタイプ

IgG2a

軽鎖のタイプ

kappa

標識

APC/Cy7®

励起波長/蛍光波長

Ex: 650nm, Em: 785nm

キャリアフリー

No

交差種

Mouse

アプリケーション

Flow Cyt

applications

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代替製品

Primary Antibodies

AB133616

Anti-CD4 antibody [EPR6855]

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [EPR6855] (AB133616)

  • 5
  • 20
Primary Antibodies

AB181724

Anti-CD4 antibody [EPR6855] - BSA and Azide free

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [EPR6855] - BSA and Azide free (AB181724)

  • 4
  • 9
Primary Antibodies

AB183685

Anti-CD4 antibody [EPR19514]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [EPR19514] (AB183685)

  • 5
  • 21
Primary Antibodies

AB196147

Alexa Fluor® 647 Anti-CD4 antibody [EPR6855]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CD4 antibody [EPR6855] (AB196147)

  • 5
  • 1
Primary Antibodies

AB196372

Alexa Fluor® 488 Anti-CD4 antibody [EPR6855]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-CD4 antibody [EPR6855] (AB196372)

  • 4
  • 2
Primary Antibodies

AB221775

Anti-CD4 antibody [EPR19514] - Low endotoxin, Azide free

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [EPR19514] - Low endotoxin, Azide free (AB221775)

  • 0
  • 0
Primary Antibodies

AB238798

Anti-CD4 antibody [SP35] - BSA and Azide free

BOND RX™ Validated|Recombinant|Advanced Validation|RabMAb

Multiplex immunohistochemistry - Anti-CD4 antibody [SP35] - BSA and Azide free (AB238798)

  • 0
  • 0
Primary Antibodies

AB277931

Alexa Fluor® 594 Anti-CD4 antibody [EPR6855]

BOND RX™ Validated|RabMAb|Recombinant|Trial Size|Trial Size|Trial Size|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 594 Anti-CD4 antibody [EPR6855] (AB277931)

  • 0
  • 0

組合せ製品

Assay Kits

AB234569

Human CD4 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human CD4 ELISA Kit (AB234569)

  • 0
  • 0
Primary Antibodies

AB24982

Cy5 ® Anti-CD45.1 antibody [A20]

  • 0
  • 0
Primary Antibodies

AB237365

Alexa Fluor® 647 Anti-CD8 alpha antibody [EPR21769]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CD8 alpha antibody [EPR21769] (AB237365)

  • 0
  • 0
Primary Antibodies

AB307134

Alexa Fluor® 488 Anti-CD3 antibody [SP162]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-CD3 antibody [SP162] (AB307134)

  • 0
  • 0
Primary Antibodies

AB272255

PE/Cy5® Anti-Interferon gamma antibody [XMG1.2]

Flow Cytometry (Intracellular) - PE/Cy5® Anti-Interferon gamma antibody [XMG1.2] (AB272255)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p>Use 0.25 μg.</p>" } } }
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製品の詳細

The RM4-5 antibody is widely used as a phenotypic marker for CD4 expression. If used together, the RM4-5 antibody and an alternative antibody, Anti-Mouse CD4 clone GK1.5, will "compete" for binding, i.e. RM4-5 antibody is able to block GK1.5 antibody binding to cells. In contrast, RM4-5 antibody does not block the binding of Anti-Mouse CD4 clone RM4-4 to cells.
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
精製に関する特記事項
ab210348 was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.
バッファー組成
pH: 7.2 Preservative: 0.09% Sodium azide Constituents: 0.87% Sodium chloride, 0.12% Sodium dihydrogen phosphate, 0.1% Gelatin
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
+4°C
保管に関する情報
Store in the dark
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule : peptide complex. The antigens presented by class II peptides are derived from extracellular proteins while class I peptides are derived from cytosolic proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class II presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of T-helper cells. In other cells such as macrophages or NK cells, plays a role in differentiation/activation, cytokine expression and cell migration in a TCR/LCK-independent pathway. Participates in the development of T-helper cells in the thymus and triggers the differentiation of monocytes into functional mature macrophages.
See full target information Cd4
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文献 (4)

Recent publications for all applications. Explore the full list and refine your search

International immunopharmacology 138:112546 PubMed38917522

2024

MC1R regulates T regulatory cell differentiation through metabolic reprogramming to promote colon cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Shaoliang Zhu,Mengjie Zou,Chunxing Li,Yuntian Tang,Honglin Luo,Xiaofeng Dong

Immunity 57:1665-1680.e7 PubMed38772365

2024

Metabolic coordination between skin epithelium and type 17 immunity sustains chronic skin inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Ipsita Subudhi,Piotr Konieczny,Aleksandr Prystupa,Rochelle L Castillo,Erica Sze-Tu,Yue Xing,Daniel Rosenblum,Ilana Reznikov,Ikjot Sidhu,Cynthia Loomis,Catherine P Lu,Niroshana Anandasabapathy,Mayte Suárez-Fariñas,Johann E Gudjonsson,Aristotelis Tsirigos,Jose U Scher,Shruti Naik

Journal of controlled release : official journal of the Controlled Release Society 294:165-175 PubMed30557650

2018

Extracellular vesicles enhance the targeted delivery of immunogenic oncolytic adenovirus and paclitaxel in immunocompetent mice.

Applications

Unspecified application

Species

Unspecified reactive species

M Garofalo,A Villa,N Rizzi,L Kuryk,B Rinner,V Cerullo,M Yliperttula,V Mazzaferro,P Ciana

Viruses 10: PubMed30322158

2018

Systemic Administration and Targeted Delivery of Immunogenic Oncolytic Adenovirus Encapsulated in Extracellular Vesicles for Cancer Therapies.

Applications

Unspecified application

Species

Unspecified reactive species

Mariangela Garofalo,Alessandro Villa,Nicoletta Rizzi,Lukasz Kuryk,Vincenzo Mazzaferro,Paolo Ciana
View all publications
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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
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