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APC Anti-CD4 抗体 [MEM-241] (ab18280)

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Flow Cytometry - APC Anti-CD4 antibody [MEM-241] (ab18280)
  • Flow Cytometry - APC Anti-CD4 antibody [MEM-241] (ab18280)

Key features and details

  • APC Mouse monoclonal [MEM-241] to CD4
  • Suitable for: Flow Cyt
  • Reacts with: Human
  • Conjugation: APC. Ex: 645nm, Em: 660nm
  • Isotype: IgG1

製品の概要

  • 製品名

    APC Anti-CD4 antibody [MEM-241]
    CD4 一次抗体 製品一覧

  • 製品の詳細

    APC Mouse monoclonal [MEM-241] to CD4

  • 由来種

    Mouse

  • 標識

    APC. Ex: 645nm, Em: 660nm

  • アプリケーション

    適用あり: Flow Cytmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Fusion protein (Fc-tag) corresponding to Human CD4. 2 N-terminal domains of human CD4 fused to human IgG1 Fc

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab18280の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt
1/20.

ab37391 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. 
特記事項
Flow Cyt
1/20.

ab37391 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. 

ターゲット情報

  • 機能

    Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.

  • 配列類似性

    Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.

  • 翻訳後修飾

    Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.

  • 細胞内局在

    Cell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum.
  • Information by UniProt

  • 参照データベース

    • 別名

      • CD 4 antibody
      • CD4 (L3T4) antibody
      • CD4 antibody
      • CD4 antigen (p55) antibody
      • CD4 antigen antibody
      • CD4 molecule antibody
      • CD4 receptor antibody
      • CD4+ Lymphocyte deficiency, included antibody
      • CD4_HUMAN antibody
      • CD4mut antibody
      • L3T4 antibody
      • Leu3 antibody
      • Ly-4 antibody
      • Lymphocyte antigen CD4 antibody
      • MGC165891 antibody
      • OTTHUMP00000238897 antibody
      • p55 antibody
      • T cell antigen T4 antibody
      • T cell antigen T4/LEU3 antibody
      • T cell differentiation antigen L3T4 antibody
      • T cell OKT4 deficiency, included antibody
      • T cell surface antigen T4/Leu 3 antibody
      • T cell surface antigen T4/Leu3 antibody
      • T cell surface glycoprotein CD4 antibody
      • T-cell surface antigen T4/Leu-3 antibody
      • T-cell surface glycoprotein CD4 antibody
      • W3/25 antibody
      • W3/25 antigen antibody
      see all

    画像

    • Flow Cytometry - APC Anti-CD4 antibody [MEM-241] (ab18280)
      Flow Cytometry - APC Anti-CD4 antibody [MEM-241] (ab18280)
      Surface staining of human peripheral blood cells with anti-human CD4 (MEM-241) APC.
    • Flow Cytometry - APC Anti-CD4 antibody [MEM-241] (ab18280)
      Flow Cytometry - APC Anti-CD4 antibody [MEM-241] (ab18280)
      Overlay histogram showing peripheral blood lymphocytes stained with ab18280 (red line). The cells were incubated with the antibody (ab18280, 1/20 dilution) for 30 min at 4ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) with secondary antibody DyLight® 649 goat anti-mouse IgG (H&L) (ab96882) at 1/200 dilution for 30 min at 4ºC. Acquisition of >5,000 events was performed gating on peripheral blood lymphocytes.

    参考文献 (1)

    ab18280 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab18280 は 1 報の論文で使用されています。

    • Wang J  et al. IRF4 overexpression promotes the transdifferentiation of tregs into macrophage-like cells to inhibit the development of colon cancer. Cancer Cell Int 21:58 (2021). PubMed: 33468159

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