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AB60902

APC Anti-CD105 抗体 [MEM-226], prediluted

APC Anti-CD105 antibody [MEM-226]

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(4 Publications)

Mouse Monoclonal CD105 antibody - conjugated to APC. Suitable for Flow Cyt and reacts with Human samples. Cited in 4 publications.

別名を表示する

CD105, END, ENG, Endoglin

1 Images
Flow Cytometry - APC Anti-CD105 antibody [MEM-226] (AB60902)
  • Flow Cyt

Supplier Data

Flow Cytometry - APC Anti-CD105 antibody [MEM-226] (AB60902)

Separation of HUVEC cells stained using ab60902 (10 ul reagent per million cells in 100 ul of cell suspension, red-filled) from HUVEC cells stained using mouse IgG2a isotype control (MOPC-173) APC antibody (concentration in sample 1 ug/ml, same as ab60902 concentration, black-dashed) in flow cytometry analysis (surface staining) of HUVEC cell suspension.

関連する標識済み抗体及び組成の異なる製品 (2)

  • 519 FITC

    FITC Anti-CD105 antibody [MEM-226]

  • 778 PE/Cy7®

    PE/Cy7® Anti-CD105 antibody [MEM-226]

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

MEM-226

アイソタイプ

IgG2a

標識

APC

励起波長/蛍光波長

Ex: 650nm, Em: 660nm

キャリアフリー

No

交差種

Human

アプリケーション

Flow Cyt

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "10 µL for 10^6 Cells", "FlowCyt-species-notes": "<p>Use 10 μl reagent / 100 μl of whole blood or 10^6 cells in a suspension.<br><br><a href='/products/primary-antibodies/apc-mouse-igg2a-b12-8-isotype-control-ab91364'>ab91364</a> - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.</p>" } } }
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Size-exclusion chromatography
精製に関する特記事項
The antibody was purified by Protein A (G) affinity chromatography before conjugation. The conjugate was purified by size-exclusion chromatography and adjusted for direct use.
バッファー組成
pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS, 0.2% BSA
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
+4°C
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

CD105 also known as endoglin or the CD105 marker is a transmembrane glycoprotein with a molecular weight of approximately 180 kDa. It is a component of the TGF-beta receptor complex and exists in endothelial cells where it is abundantly expressed. Expression of CD105 is higher in proliferating cells particularly in the vasculature. You can also find it in tissues involved in the formation and remodeling of blood vessels such as during angiogenesis.
Biological function summary

Endoglin functions in the regulation of angiogenesis and vascular remodeling. It plays a significant role in mediating cellular responses to TGF-beta signaling influencing endothelial cell proliferation and migration. While not part of a larger structural complex endoglin interacts with receptors and signaling molecules important for vascular development and repair processes. This involvement aids in maintaining endothelial integrity and function under various physiological conditions.

Pathways

CD105 participates in the TGF-beta signaling and angiogenesis pathways. In these pathways it acts in conjunction with other proteins like TGF-beta receptors which play roles in cell differentiation proliferation and apoptosis. The interaction between CD105 and TGF-beta signaling regulates numerous cellular mechanisms impacting angiogenesis and cellular responses to environmental changes.

CD105 has links to hereditary hemorrhagic telangiectasia (HHT) and certain cancers. In HHT mutations in the endoglin gene alter vascular structure leading to the formation of abnormal blood vessels. Oncologically overexpression of CD105 is present in tumor angiogenesis aiding in the progression of certain cancers. Other proteins like VEGF and TGF-beta closely interact with endoglin influencing disease progression and presenting potential targets for therapeutic intervention.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Vascular endothelium glycoprotein that plays an important role in the regulation of angiogenesis (PubMed : 21737454, PubMed : 23300529). Required for normal structure and integrity of adult vasculature (PubMed : 7894484). Regulates the migration of vascular endothelial cells (PubMed : 17540773). Required for normal extraembryonic angiogenesis and for embryonic heart development (By similarity). May regulate endothelial cell shape changes in response to blood flow, which drive vascular remodeling and establishment of normal vascular morphology during angiogenesis (By similarity). May play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors (PubMed : 1692830). Acts as a TGF-beta coreceptor and is involved in the TGF-beta/BMP signaling cascade that ultimately leads to the activation of SMAD transcription factors (PubMed : 21737454, PubMed : 22347366, PubMed : 23300529, PubMed : 8370410). Required for GDF2/BMP9 signaling through SMAD1 in endothelial cells and modulates TGFB1 signaling through SMAD3 (PubMed : 21737454, PubMed : 22347366, PubMed : 23300529).
See full target information ENG
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文献 (4)

Recent publications for all applications. Explore the full list and refine your search

Journal of tissue engineering and regenerative med 13:433-445 PubMed30650247

2019

Effect of donor variation on osteogenesis and vasculogenesis in hydrogel cocultures.

Applications

Unspecified application

Species

Unspecified reactive species

Iris Pennings,Lukas A van Dijk,Juliet van Huuksloot,Joost O Fledderus,Koen Schepers,A Koen Braat,Edward C Hsiao,Emilie Barruet,Blanca M Morales,Marianne C Verhaar,Antoine J W P Rosenberg,Debby Gawlitta

Journal of immunology (Baltimore, Md. : 1950) 200:2542-2553 PubMed29531170

2018

rIgG1 Fc Hexamer Inhibits Antibody-Mediated Autoimmune Disease via Effects on Complement and FcγRs.

Applications

Unspecified application

Species

Unspecified reactive species

Rolf Spirig,Ian K Campbell,Sandra Koernig,Chao-Guang Chen,Bonnie J B Lewis,Rebecca Butcher,Ineke Muir,Shirley Taylor,Jenny Chia,David Leong,Jason Simmonds,Pierre Scotney,Peter Schmidt,Louis Fabri,Andreas Hofmann,Monika Jordi,Martin O Spycher,Susann Cattepoel,Jennifer Brasseit,Con Panousis,Tony Rowe,Donald R Branch,Adriana Baz Morelli,Fabian Käsermann,Adrian W Zuercher

Nature methods 10:77-83 PubMed23202434

2012

Conversion of human fibroblasts to angioblast-like progenitor cells.

Applications

Unspecified application

Species

Unspecified reactive species

Leo Kurian,Ignacio Sancho-Martinez,Emmanuel Nivet,Aitor Aguirre,Krystal Moon,Caroline Pendaries,Cecile Volle-Challier,Francoise Bono,Jean-Marc Herbert,Julian Pulecio,Yun Xia,Mo Li,Nuria Montserrat,Sergio Ruiz,Ilir Dubova,Concepcion Rodriguez,Ahmet M Denli,Francesca S Boscolo,Rathi D Thiagarajan,Fred H Gage,Jeanne F Loring,Louise C Laurent,Juan Carlos Izpisua Belmonte

The Journal of clinical investigation 121:3206-19 PubMed21737876

2011

Human ovarian carcinoma–associated mesenchymal stem cells regulate cancer stem cells and tumorigenesis via altered BMP production.

Applications

Flow Cyt

Species

Human

Karen McLean,Yusong Gong,Yunjung Choi,Ning Deng,Kun Yang,Shoumei Bai,Lourdes Cabrera,Evan Keller,Laurie McCauley,Kathleen R Cho,Ronald J Buckanovich
View all publications
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組合せ製品

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