AB317754

APC Anti-beta Tubulin 抗体 [EPR16774]

APC Anti-beta Tubulin antibody [EPR16774] - Microtubule Marker

Recombinant
RabMAb
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Rabbit Recombinant Monoclonal Beta-5-tubulin antibody - conjugated to APC. Suitable for ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, African green monkey, Rat samples.

別名を表示する

TUBB5, OK/SW-cl.56, TUBB, Tubulin beta chain, Tubulin beta-5 chain

6 Images

Flow Cytometry (Intracellular) - APC Anti-beta Tubulin antibody [EPR16774] - Microtubule Marker (AB317754)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - APC Anti-beta Tubulin antibody [EPR16774] - Microtubule Marker (AB317754)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa(human cervical adenocarcinoma epithelial cell) cells labelling beta Tubulin with ab317754 at 1/500 dilution(0.1ug)/Red (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Allophycocyanin) (ab232814) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Immunocytochemistry/ Immunofluorescence - APC Anti-beta Tubulin antibody [EPR16774] - Microtubule Marker (AB317754)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - APC Anti-beta Tubulin antibody [EPR16774] - Microtubule Marker (AB317754)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling beta Tubulin with ab317754 at 1/50 (10.0 ug/ml) dilution (Magenta).

Confocal image showing cytoplasmic staining in HeLa cell line (shown in magenta). The counterstain was observed in green. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Green). The Nuclear counterstain was DAPI (Blue).

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - APC Anti-beta Tubulin antibody [EPR16774] - Microtubule Marker (AB317754)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling beta Tubulin with ab317754 at 1/50 (10.0 ug/ml) dilution (Magenta).

Confocal image showing cytoplasmic staining in NIH/3T3 cell line (shown in magenta). The counterstain was observed in green. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - APC Anti-beta Tubulin antibody [EPR16774] - Microtubule Marker (AB317754)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling beta Tubulin with ab317754 at 1/500 dilution(0.1ug) (Red) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Allophycocyanin) (ab232814) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - APC Anti-beta Tubulin antibody [EPR16774] - Microtubule Marker (AB317754)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized COS-1 (African green monkey kidney fibroblast-like cell) cells labelling beta Tubulin with ab317754 at 1/500 dilution(0.1ug) (Red) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Allophycocyanin) (ab232814) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - APC Anti-beta Tubulin antibody [EPR16774] - Microtubule Marker (AB317754)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized COS-1 (African green monkey kidney fibroblast-like) cells labelling beta Tubulin with ab317754 at 1/50 (10.0 ug/ml) dilution (Magenta).

Confocal image showing cytoplasmic staining in COS-1 cell line (shown in magenta). The counterstain was observed in green. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR16774

アイソタイプ

IgG

標識

APC

励起波長/蛍光波長

Ex: 650nm, Em: 660nm

キャリアフリー

交差種

Human, Mouse, African green monkey, Human, Mouse, Rat

アプリケーション

ICC/IF, IHC-P, Flow Cyt (Intra)

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "African green monkey": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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製品の詳細

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

+4°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle|Store in the dark

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Beta tubulin also known as beta-tubulin or β-tubulin is a globular protein with a molecular weight of approximately 50 kDa. It is a major component of microtubules which are cylindrical structures vital for maintaining cell shape and enabling intracellular transport. This protein is expressed widely in eukaryotic cells and plays a role in mitosis and meiosis by forming the spindle apparatus important for chromosome separation during cell division. The size of beta tubulin allows it to effectively polymerize with alpha tubulin forming heterodimers which are the building blocks of the microtubule network.

Biological function summary

This protein contributes significantly to the dynamic instability of microtubules allowing rapid assembly and disassembly which is essential for cellular processes like motility signaling and maintaining the cell’s architecture. Beta tubulin operates as part of the tubulin family which includes several related proteins within microtubule structures. The beta-tubulin molecules in microtubules are critical for the interactions with microtubule-associated proteins (MAPs) and motor proteins such as kinesin and dynein influencing trafficking and positioning of organelles within the cell.

Pathways

Beta tubulin plays key roles in the mitotic spindle assembly checkpoint ensuring accurate chromosome segregation. It is actively involved in the microtubule pathway and has associations with signaling pathways such as the Wnt signaling pathway which affects cell growth and differentiation. Through these pathways beta tubulin interacts with proteins like tau and MAP2 which stabilize microtubules to control their functional dynamics within the cell.

Mutations or dysregulations in beta tubulin can affect neurological and proliferative conditions such as neurodegenerative diseases and cancer. Beta tubulin is notably linked to Alzheimer's disease where tau protein tangles disrupt normal microtubule function. It also connects to diseases like paclitaxel-resistant cancer where altered beta tubulin isoform expression can lead to chemotherapy resistance. The interaction of beta tubulin with tau and alpha tubulin provides further insight into etiological mechanisms of these conditions.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.

See full target information TUBB

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

APC Anti-beta Tubulin 抗体 [EPR16774]

APC Anti-beta Tubulin antibody [EPR16774] - Microtubule Marker

Flow Cytometry (Intracellular) - APC Anti-beta Tubulin antibody [EPR16774] - Microtubule Marker (AB317754)

Immunocytochemistry/ Immunofluorescence - APC Anti-beta Tubulin antibody [EPR16774] - Microtubule Marker (AB317754)

Immunocytochemistry/ Immunofluorescence - APC Anti-beta Tubulin antibody [EPR16774] - Microtubule Marker (AB317754)

Flow Cytometry (Intracellular) - APC Anti-beta Tubulin antibody [EPR16774] - Microtubule Marker (AB317754)

Flow Cytometry (Intracellular) - APC Anti-beta Tubulin antibody [EPR16774] - Microtubule Marker (AB317754)

Immunocytochemistry/ Immunofluorescence - APC Anti-beta Tubulin antibody [EPR16774] - Microtubule Marker (AB317754)

関連する標識済み抗体及び組成の異なる製品 (10)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

標識

励起波長/蛍光波長

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

組合せ製品

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise