Anti-APAF1 抗体 (ab46899)
Key features and details
- Rabbit polyclonal to APAF1
- Suitable for: IHC-FoFr
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-APAF1 antibody
APAF1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to APAF1 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-FoFrmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Synthetic peptide within Human APAF1 aa 1200 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: O14727 -
ポジティブ･コントロール
- Testis
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特記事項
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab46899の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-FoFr |
1/200.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. |
| 特記事項 |
|---|
|
IHC-FoFr
1/200. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. |
ターゲット情報
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機能
Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis. -
組織特異性
Ubiquitous. Highest levels of expression in adult spleen and peripheral blood leukocytes, and in fetal brain, kidney and lung. Isoform 1 is expressed in heart, kidney and liver. -
配列類似性
Contains 1 CARD domain.
Contains 1 NB-ARC domain.
Contains 13 WD repeats. -
ドメイン
The CARD domain mediates interaction with APIP. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 317 Human
- Entrez Gene: 11783 Mouse
- Entrez Gene: 78963 Rat
- Omim: 602233 Human
- SwissProt: O14727 Human
- SwissProt: O88879 Mouse
- SwissProt: Q9EPV5 Rat
- Unigene: 728891 Human
see all -
別名
- APAF 1 antibody
- Apaf-1 antibody
- APAF_HUMAN antibody
see all
画像
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Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-APAF1 antibody (ab46899)Human testis stained with ab46899 using the antibody at a dilution of 1:200
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab46899 は論文での使用が確認できていません。