Anti-AP3M2 抗体 [EPR12965] - N-terminal (ab180138)
![Western blot - Anti-AP3M2 antibody [EPR12965] - N-terminal (ab180138)](https://www.abcam.co.jp/ps/products/180/ab180138/Images/ab180138-209962-ab180138.jpg "Western blot - Anti-AP3M2 antibody [EPR12965] - N-terminal (ab180138)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12965] to AP3M2 - N-terminal
- Suitable for: Flow Cyt (Intra), WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-AP3M2 antibody [EPR12965] - N-terminal
AP3M2 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR12965] to AP3M2 - N-terminal -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), WB, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Synthetic peptide within Human AP3M2 aa 1-100 (N terminal). The exact sequence is proprietary.
Database link: P53677 -
ポジティブ･コントロール
- Human fetal brain, HepG2, SH-SY5Y and U87-MG lysates; HepG2 cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
EPR12965 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab180138の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt (Intra) |
1/30.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| WB |
1/5000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 47 kDa).
|
|
| ICC/IF |
1/500.
|
| 特記事項 |
|---|
|
Flow Cyt (Intra)
1/30. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/5000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 47 kDa). |
|
ICC/IF
1/500. |
ターゲット情報
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関連性
Part of the AP-3 complex, AP3M3 is an adaptor-related complex not clathrin-associated. The complex is associated with the Golgi region and to more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to lysosomes. -
細胞内局在
Golgi apparatus. -
参照データベース
- Entrez Gene: 10947 Human
- Entrez Gene: 64933 Mouse
- Entrez Gene: 140667 Rat
- Omim: 610469 Human
- SwissProt: P53677 Human
- SwissProt: Q8R2R9 Mouse
- SwissProt: P53678 Rat
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別名
- Adapter related protein complex 3 mu 2 subunit antibody
- AP-3 complex subunit mu 2 antibody
- Clathrin assembly protein assembly protein complex 1 medium chain homolog 2 antibody
see all
画像
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Western blot - Anti-AP3M2 antibody [EPR12965] - N-terminal (ab180138)All lanes : Anti-AP3M2 antibody [EPR12965] - N-terminal (ab180138) at 1/20000 dilution
Lane 1 : Human fetal brain tissue lysate
Lane 2 : HepG2 cell Lysate
Lane 3 : SH-SY5Y cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 47 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-AP3M2 antibody [EPR12965] - N-terminal (ab180138)](https://www.abcam.co.jp/ps/products/180/ab180138/Images/ab180138-209967-ab1801383.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-AP3M2 antibody [EPR12965] - N-terminal (ab180138)Immunofluorescent analysis of 4% paraformaldehyde-fixed HepG2 cells labeling AP3M2 with ab180138 at 1/500 dilution. Secondary antibody Goat anti rabbit IgG (Dylight 488) at 1/250 dilution. Conterstained with Dapi blue.
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![Flow Cytometry (Intracellular) - Anti-AP3M2 antibody [EPR12965] - N-terminal (ab180138)](https://www.abcam.co.jp/ps/products/180/ab180138/Images/ab180138-209968-ab1801384.jpg)
Flow Cytometry (Intracellular) - Anti-AP3M2 antibody [EPR12965] - N-terminal (ab180138)Intracellular flow cytometric analysis of 2% paraformaldehyde fixed U87-MG cells labeling AP3M2 with ab180138 at 1/30 dilution. Secondary antibody Goat anti rabbit IgG (FITC) at 1/75 dilution. Isotype control Rabbit IgG.
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![Western blot - Anti-AP3M2 antibody [EPR12965] - N-terminal (ab180138)](https://www.abcam.co.jp/ps/products/180/ab180138/Images/ab180138-209966-ab1801382.jpg)
Western blot - Anti-AP3M2 antibody [EPR12965] - N-terminal (ab180138)Anti-AP3M2 antibody [EPR12965] - N-terminal (ab180138) at 1/5000 dilution + U87-MG at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 47 kDa -
![Anti-AP3M2 antibody [EPR12965] - N-terminal (ab180138)](https://www.abcam.co.jp/ps/products/180/ab180138/Images/ab180138-5-benefits-of-recombinant-antibodies.png)
Anti-AP3M2 antibody [EPR12965] - N-terminal (ab180138)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab180138 は論文での使用が確認できていません。