Anti-AP3B2 抗体 (ab113100)
Key features and details
- Rabbit polyclonal to AP3B2
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Rat
- Isotype: IgG
製品の概要
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製品名
Anti-AP3B2 antibody -
製品の詳細
Rabbit polyclonal to AP3B2 -
由来種
Rabbit -
特異性
At least three isoforms of AP3B2 are known to exist; this antibody will recognize the two larger isoforms. -
アプリケーション
適用あり: WB, ICC/IF, IHC-Pmore details -
種交差性
交差種: Mouse, Rat -
免疫原
An 18 amino acid synthetic peptide from near the amino terminus of Human AP3B2 (NP_004635).
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ポジティブ･コントロール
- WB: Rat brain tissue lysate, mouse brain lysate, IHC-P: Mouse brain tissue. ICC: Mouse brain tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at 4°C (stable for up to 12 months). -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: 99.98% PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab113100 is purified by affinity chromatography via a peptide column. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab113100の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 119 kDa.
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| ICC/IF |
Use a concentration of 20 µg/ml.
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| IHC-P |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
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WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 119 kDa. |
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ICC/IF
Use a concentration of 20 µg/ml. |
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IHC-P
Use at an assay dependent concentration. |
ターゲット情報
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機能
Subunit of non-clathrin- and clathrin-associated adaptor protein complex 3 that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules. AP-3 appears to be involved in the sorting of a subset of transmembrane proteins targeted to lysosomes and lysosome-related organelles. -
組織特異性
Expression is specific to nervous system. Expressed in nerve terminal and cell body, and is associated with nerve-terminal vesicles. Expression seen in Purkinje cells, cortical neurons, neuroectodermal tumors and graded in cerebral cortex (deeper layers exhibit stronger expression). -
配列類似性
Belongs to the adaptor complexes large subunit family. -
発生段階
Expressed from early in development through adulthood. -
細胞内局在
Golgi apparatus. Cytoplasmic vesicle > clathrin-coated vesicle membrane. Golgi apparatus. Component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex. - Information by UniProt
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参照データベース
- Entrez Gene: 11775 Mouse
- Entrez Gene: 308777 Rat
- SwissProt: Q9JME5 Mouse
- Unigene: 322894 Mouse
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別名
- Adapter-related protein complex 3 subunit beta-2 antibody
- Adaptor protein complex AP-3 subunit beta-2 antibody
- Adaptor related protein complex 3 beta 2 subunit antibody
see all
画像
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Western blot - Anti-AP3B2 antibody (ab113100)Western Blot of rat brain tissue lysate labeling AP3B2 with Anti-AP3B2 antibody (ab113100) at (1) 1 and (2) 2µg/ml.
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Immunocytochemistry/ Immunofluorescence - Anti-AP3B2 antibody (ab113100)Immunofluorescence of AP3B2 in mouse brain tissue with ab113100 at 20 ug/mL. -
Western blot - Anti-AP3B2 antibody (ab113100)Lane 1 : Anti-AP3B2 antibody (ab113100) at 1 µg/ml
Lane 2 : Anti-AP3B2 antibody (ab113100) at 2 µg/ml
All lanes : Mouse brain lysate
Predicted band size: 119 kDa -
Western blot - Anti-AP3B2 antibody (ab113100)Lane 1 : Anti-AP3B2 antibody (ab113100) at 1 µg/ml
Lane 2 : Anti-AP3B2 antibody (ab113100) at 2 µg/ml
All lanes : Rat brain tissue lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 119 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP3B2 antibody (ab113100)Immunohistochemical analysis of mouse brain tissue, staining AP3B2 with ab113100 at 5 µg/ml.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab113100 は論文での使用が確認できていません。