JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB151961

Anti-AP2B1 抗体

Anti-AP2B1 antibody

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Polyclonal AP2B1 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human AP2B1 aa 300 to C-terminus.

別名を表示する

ADTB2, CLAPB1, AP2B1, AP-2 complex subunit beta, AP105B, Adaptor protein complex AP-2 subunit beta, Adaptor-related protein complex 2 subunit beta, Beta-2-adaptin, Beta-adaptin, Clathrin assembly protein complex 2 beta large chain, Plasma membrane adaptor HA2/AP2 adaptin beta subunit

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP2B1 antibody (AB151961)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP2B1 antibody (AB151961)

Immunohistochemical analysis of paraffin embedded Human lung adenocarcinoma tissue labeling AP2B1 with ab151961 antibody at 1/250.

Western blot - Anti-AP2B1 antibody (AB151961)
  • WB

Unknown

Western blot - Anti-AP2B1 antibody (AB151961)

7.5% SDS PAGE

All lanes:

Western blot - Anti-AP2B1 antibody (ab151961) at 1/1000 dilution

All lanes:

IMR32 whole cell lysate at 30 µg

Predicted band size: 105 kDa

false

Western blot - Anti-AP2B1 antibody (AB151961)
  • WB

Unknown

Western blot - Anti-AP2B1 antibody (AB151961)

7.5% SDS PAGE

All lanes:

Western blot - Anti-AP2B1 antibody (ab151961) at 1/1000 dilution

All lanes:

Mouse brain whole cell lysate at 50 µg

Predicted band size: 105 kDa

false

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

No

交差種

Mouse, Human

アプリケーション

IHC-P, WB

applications

免疫原

Recombinant Fragment Protein within Human AP2B1 aa 300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P63010

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus tropicalis": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Zebrafish": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
style
left-column
style
left-column

出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Immunogen
バッファー組成
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
style
left-column

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

AP2B1 also known as the beta-1 subunit of the adaptor protein complex 2 (AP-2 complex) has a mass of approximately 105 kDa. It plays an important role in clathrin-mediated endocytosis by forming a bridge between clathrin and cargo molecules. The AP-2 complex is essential for selecting and concentrating cargo proteins that are destined for endocytosis. AP2B1 is highly expressed in most tissues with abundant presence in brain tissue suggesting its importance in neural processes.
Biological function summary

AP2B1 functions as a component of the AP-2 complex which is essential for the formation of clathrin-coated pits on the plasma membrane. It contributes to the regulation of receptor-mediated endocytosis a process important for nutrient uptake and signaling receptor regulation. The AP-2 complex binds to the cytoplasmic tails of transmembrane proteins mediating their internalization into cells for degradation or recycling. This highlights AP2B1's role in maintaining cellular homeostasis and responding to environmental signals.

Pathways

Researchers have shown that AP2B1 is actively involved in the endocytic pathway facilitating the internalization of cell surface receptors into vesicles. It is also linked to the sorting of cargo proteins within the endocytosis pathway in tandem with other proteins like calmodulin. The interaction with dynamin regulates the membrane scission events necessary for vesicle release. Both calmodulin and dynamin are important for the successful execution of these pathways where AP2B1 plays a central role.

Disruptions in AP2B1 function link to neurological disorders such as Alzheimer’s disease potentially due to impaired endocytosis and subsequent cellular signaling dysfunctions. Abnormalities in the AP-2 complex including AP2B1 have also been associated with cancers like melanoma where altered receptor endocytosis can lead to uncontrolled cell signaling and proliferation. Both conditions involve pathways where dynamin works closely with AP2B1 hinting at its wider implications in disease progression and therapeutic target potential.
style
left-column
style
left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

ターゲットの情報

Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. During long-term potentiation in hippocampal neurons, AP-2 is responsible for the endocytosis of ADAM10 (PubMed : 23676497). The AP-2 beta subunit acts via its C-terminal appendage domain as a scaffolding platform for endocytic accessory proteins; at least some clathrin-associated sorting proteins (CLASPs) are recognized by their [DE]-X(1,2)-F-X-X-[FL]-X-X-X-R motif. The AP-2 beta subunit binds to clathrin heavy chain, promoting clathrin lattice assembly; clathrin displaces at least some CLASPs from AP2B1 which probably then can be positioned for further coat assembly.
See full target information AP2B1
style
left-column

文献 (1)

Recent publications for all applications. Explore the full list and refine your search

eLife 7: PubMed29345618

2018

NECAPs are negative regulators of the AP2 clathrin adaptor complex.

Applications

Unspecified application

Species

Unspecified reactive species

Gwendolyn M Beacham,Edward A Partlow,Jeffrey J Lange,Gunther Hollopeter
View all publications
style
left-column
style
left-column
style
right-column

代替製品

Primary Antibodies

AB220778

Anti-AP2B1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-AP2B1 antibody (AB220778)

  • 0
  • 0
Primary Antibodies

AB129168

Anti-AP2B1 antibody [EPR7567]

RabMAb|Recombinant

Western blot - Anti-AP2B1 antibody [EPR7567] (AB129168)

  • 0
  • 0
Primary Antibodies

AB205014

Anti-AP2B1 antibody

Immunoprecipitation - Anti-AP2B1 antibody (AB205014)

  • 0
  • 0

組合せ製品

Primary Antibodies

AB128950

Anti-AP2S1 antibody [EPR2697(2)]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP2S1 antibody [EPR2697(2)] (AB128950)

  • 2
  • 1
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB75748

Anti-EEF2/Elongation factor 2 antibody [EP880Y]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EEF2/Elongation factor 2 antibody [EP880Y] (AB75748)

  • 0
  • 0
Primary Antibodies

AB21679

Anti-Clathrin heavy chain antibody

Western blot - Anti-Clathrin heavy chain antibody (AB21679)

  • 5
  • 38

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com