Name: Anti-AP2 gamma/TFAP2C antibody [EP2692Y]
Brand: RabMAb
SKU: ab76007
Rating: 5 (1 reviews)

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Western blot - Anti-AP2 gamma/TFAP2C antibody [EP2692Y] (ab76007)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP2692Y] to AP2 gamma/TFAP2C
Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
Reacts with: Human

Carrier Free

製品名

Anti-AP2 gamma/TFAP2C antibody [EP2692Y]
AP2 gamma/TFAP2C 一次抗体製品一覧
製品の詳細

Rabbit monoclonal [EP2692Y] to AP2 gamma/TFAP2C
由来種

Rabbit
アプリケーション

適用あり: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details
適用なし: IP
種交差性

交差種: Human
免疫原

Synthetic peptide within Human AP2 gamma/TFAP2C aa 100-200. The exact sequence is proprietary.
ポジティブ･コントロール
- WB: MCF7 and SKBR3 cell lysates. IHC-P: Human cervical carcinoma, endometrial carcinoma and prostatic carcinoma tissues. ICC/IF: MCF7 cells Flow Cyt (intra): MCF7 cells
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の状態

Liquid
保存方法

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
バッファー

pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
精製度

Protein A purified
ポリ/モノ

モノクローナル
クローン名

EP2692Y
アイソタイプ

IgG
研究分野

Alternative Versions
- Anti-AP2 gamma/TFAP2C antibody [EP2692Y] - BSA and Azide free (ab247427)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Positive Controls
- MCF7 nuclear extract lysate (ab14860)

The Abpromise guarantee

Abpromise保証は、次のテスト済みアプリケーションにおけるab76007の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション	Abreviews	特記事項
Flow Cyt (Intra)		1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB	(1)	1/25000 - 1/50000. Predicted molecular weight: 49 kDa.
IHC-P		1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF		1/500.

特記事項
Flow Cyt (Intra) 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB 1/25000 - 1/50000. Predicted molecular weight: 49 kDa.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/500.

追加情報

Is unsuitable for IP.

機能

Sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC.
配列類似性

Belongs to the AP-2 family.
ドメイン

The WW-binding motif mediates interaction with WWOX.
翻訳後修飾

Sumoylated on Lys-10; which inhibits transcriptional activity.
細胞内局在

Nucleus.
Target information above from: UniProt accession Q92754 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
参照データベース
- Entrez Gene: 7022 Human
- Omim: 601602 Human
- SwissProt: Q92754 Human
- Unigene: 473152 Human
別名
- Activating enhancer binding protein 2 gamma antibody
- Activating enhancer-binding protein 2 gamma antibody
- AP2-gamma antibody
- AP2C_HUMAN antibody
- AP2g antibody
- AP2gamma antibody
- ERF 1 antibody
- ERF1 antibody
- Estrogen receptor factor 1 antibody
- hAP 2g antibody
- hAP2g antibody
- TFAP 2C antibody
- TFAP 2G antibody
- Tfap2c antibody
- TFAP2G antibody
- Transcription factor AP 2 gamma (activating enhancer binding protein 2 gamma) antibody
- Transcription factor AP 2 gamma antibody
- Transcription factor AP-2 gamma antibody
- Transcription factor ERF 1 antibody
- Transcription factor ERF-1 antibody
- Transcription factor ERF1 antibody
see all

Western blot - Anti-AP2 gamma/TFAP2C antibody [EP2692Y] (ab76007)

All lanes : Anti-AP2 gamma/TFAP2C antibody [EP2692Y] (ab76007) at 1/50000 dilution

Lane 1 : MCF7 cell lysate
Lane 2 : SKBR3 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-rabbit HRP at 1/1000 dilution

Predicted band size: 49 kDa
Observed band size: 49 kDa
Immunocytochemistry/ Immunofluorescence - Anti-AP2 gamma/TFAP2C antibody [EP2692Y] (ab76007)

Immunocytochemistry/ Immunofluorescence analysis of MCF7 (human breast adenocarcinoma epithelial cell) cells labeling AP2 gamma/TFAP2C with purified ab76007 at 1/500 dilution (2 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1/200 (2.5 µg/mL) was used as the secondary antibody only control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP2 gamma/TFAP2C antibody [EP2692Y] (ab76007)

ab76007 showing positive staining in Cervical carcinoma tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cytometry (Intracellular) - Anti-AP2 gamma/TFAP2C antibody [EP2692Y] (ab76007)

Intracellular Flow Cytometry analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling AP2 gamma/TFAP2C with purified ab76007 at 1/100 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cells without incubation with primary antibody and secondary antibody (Blue).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP2 gamma/TFAP2C antibody [EP2692Y] (ab76007)

ab76007 showing positive staining in Endometrial carcinoma tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP2 gamma/TFAP2C antibody [EP2692Y] (ab76007)

ab76007 showing positive staining in Prostatic carcinoma tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-AP2 gamma/TFAP2C antibody [EP2692Y] (ab76007)

Flow cytometry protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

ab76007 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab76007 は 5 報の論文で使用されています。

Zhang Y et al. Epidermal growth factor induces a trophectoderm lineage transcriptome resembling that of human embryos during reconstruction of blastoids from extended pluripotent stem cells. Cell Prolif 55:e13317 (2022). PubMed: 35880490
Lin B et al. Smurf2-induced degradation of SMAD2 causes inhibition of hair follicle stem cell differentiation. Cell Death Discov 8:160 (2022). PubMed: 35379779
Wu VT et al. A TFAP2C Gene Signature Is Predictive of Outcome in HER2-Positive Breast Cancer. Mol Cancer Res 18:46-56 (2020). PubMed: 31619506
Chen D et al. The TFAP2C-Regulated OCT4 Naive Enhancer Is Involved in Human Germline Formation. Cell Rep 25:3591-3602.e5 (2018). PubMed: 30590035
Pastor WA et al. TFAP2C regulates transcription in human naive pluripotency by opening enhancers. Nat Cell Biol 20:553-564 (2018). PubMed: 29695788

レビューと Q&A

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レビューを送る質問を送る

1-2 of 2 Abreviews or Q&A

Application

Western blot

Sample

Human Cell lysate - whole cell (Human ESC)

Gel Running Conditions

Reduced Denaturing

Loading amount

500000 cells

Specification

Human ESC

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Timothy Hunt

Verified customer

投稿 Aug 22 2017

Question

Will this antibody cross react with AP2 alpha, beta, or epsilon?

Abcam community

Verified customer

Asked on Feb 22 2012

Answer

Thank you for your call today and for your question about ab76007.
I've heard back from the lab, and this antibody is not predicted to cross react with AP2 alpha, beta, or epsilon. The exact immunogen shares 60%, 60%, and ˜0% identity with these AP2 family members.
I hope this information will be useful, but please let me know if you have any further questions and I'll be happy to help.

Abcam Scientific Support

Answered on Feb 22 2012

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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Key features and details

Related conjugates and formulations

製品の概要

製品名

製品の詳細

由来種

アプリケーション

種交差性

免疫原

ポジティブ･コントロール

特記事項

製品の特性

製品の状態

保存方法

バッファー

精製度

ポリ/モノ

クローン名

アイソタイプ

研究分野

関連製品

Alternative Versions

Isotype control

Positive Controls

アプリケーション

The Abpromise guarantee

ターゲット情報

機能

配列類似性

ドメイン

翻訳後修飾

細胞内局在

参照データベース

別名

画像

プロトコール

データシートおよび資料

SDS download

Datasheet download

参考文献 (5)

レビューと Q&A

Western blot abreview for Anti-AP2 gamma antibody [EP2692Y]