AB217055

Anti-AP1S1 抗体

Name: Anti-AP1S1 抗体 (ab217055) | Abcam
Brand: Abcam Limited
SKU: AB217055
Price: 74000 JPY
Availability: InStock

Anti-AP1S1 antibody

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(2 Publications)

Rabbit Polyclonal AP1S1 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human AP1S1 aa 100 to C-terminus.

別名を表示する

AP19, CLAPS1, AP1S1, AP-1 complex subunit sigma-1A, Adaptor protein complex AP-1 subunit sigma-1A, Adaptor-related protein complex 1 subunit sigma-1A, Clathrin assembly protein complex 1 sigma-1A small chain, Clathrin coat assembly protein AP19, Golgi adaptor HA1/AP1 adaptin sigma-1A subunit, HA1 19 kDa subunit, Sigma 1a subunit of AP-1 clathrin, Sigma-adaptin 1A, Sigma1A-adaptin

2 Images

Unknown

Immunoprecipitation - Anti-AP1S1 antibody (AB217055)

Immunoprecipitation analysis of 293T cell lysate (in NETN buffer, 0.5 or 1.0 mg per IP reaction; 20% of IP loaded) labeling AP1S1 using ab217055 at 6 μg per reaction (lane 1). A Control IgG was used in lane 2. For blotting immunoprecipitated AP1S1, ab217055 was used at 1 μg/ml. Detection : Chemiluminescence with an exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-AP1S1 antibody (ab217055)

Predicted band size: 19 kDa

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Supplier Data

Western blot - Anti-AP1S1 antibody (AB217055)

cells prepared using NETN lysis buffer

All lanes:

Western blot - Anti-AP1S1 antibody (ab217055) at 0.1 µg/mL

Lane 1:

HeLa cell lysate at 15 µg

Lane 2:

293T cell lysate at 15 µg

Lane 3:

Jurkat cell lysate at 15 µg

Lane 4:

TCMK1 cell lysate at 15 µg

Lane 5:

NIH 3T3 cell lysate at 15 µg

Predicted band size: 19 kDa

true

Exposure time: 10s

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Mouse, Human

アプリケーション

WB, IP

applications

免疫原

Synthetic Peptide within Human AP1S1 aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P61966

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

精製に関する特記事項

ab217055 was affinity purified using an epitope specific to AP1S1 immobilized on solid support.

バッファー組成

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

+4°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

AP1S1 also known as Adaptor Protein complex 1 Sigma 1 is an integral component of the AP-1 adaptor complex. This complex is involved in the sorting and trafficking of transmembrane proteins. AP1S1 has a molecular mass of approximately 19 kDa and is universally expressed in various tissues including the liver and brain. Its main function involves the interaction with clathrin aiding in the selection of cargo proteins for transport between the trans-Golgi network and endosomes.

Biological function summary

AP1S1 plays a significant role in vesicular trafficking processes. It participates as part of the AP-1 adaptor protein complex which is responsible for mediating sorting signals in coated vesicles. Through this participation AP1S1 influences the efficiency and specificity of intracellular transport. The AP-1 complex also interacts with phosphatidylinositol phosphates further regulating the vesicle formation and protein sorting mechanisms within cells. This protein's involvement is critical for maintaining cellular homeostasis and specific protein function.

Pathways

AP1S1 functions in pathways related to protein sorting and vesicular transport. It plays a role within the clathrin-mediated endocytosis pathway important for cellular receptor regulation and nutrient uptake. AP1S1 connects with other proteins such as clathrin and other adaptor proteins to form a network ensuring efficient cargo selection and transportation. This function ensures a streamlined process for the cellular uptake and regulation of essential molecules.

AP1S1 has links to various conditions through its role in protein trafficking. Mutations in AP1S1 associate with Mednik Syndrome a disorder characterized by neurological issues due to improper protein sorting. Another connection involves its association with hereditary spastic paraplegia where misregulation of protein trafficking results in neurological deterioration. In these contexts AP1S1 interacts with proteins like AP1B1 which similarly impacts vesicular transport and sorting pathways affecting overall cellular functionality.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.

See full target information AP1S1

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Human cell 36:812-821 PubMed36629984

2023

SPOCK2 and SPRED1 function downstream of EZH2 to impede the malignant progression of lung adenocarcinoma in vitro and in vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Liu,Xiaoxi Fan,Changrui Jiang,Shun Xu

Human genetics 139:1247-1259 PubMed32306098

2020

AP1S1 missense mutations cause a congenital enteropathy via an epithelial barrier defect.

Applications

Unspecified application

Species

Unspecified reactive species

Katharina M C Klee,Andreas R Janecke,Hasret A Civan,Štefan Rosipal,Peter Heinz-Erian,Lukas A Huber,Thomas Müller,Georg F Vogel

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-AP1S1 抗体

Anti-AP1S1 antibody

Immunoprecipitation - Anti-AP1S1 antibody (AB217055)

Western blot - Anti-AP1S1 antibody (AB217055)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

精製に関する特記事項

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (2)

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise