Anti-Anterior Gradient 2 抗体 [EPR3278] (ab76473)
![Western blot - Anti-Anterior Gradient 2 antibody [EPR3278] (ab76473)](https://www.abcam.co.jp/ps/products/76/ab76473/Images/ab76473-91912-anti-anterior-gradient-2-antibody-epr3278-western-blot.jpg "Western blot - Anti-Anterior Gradient 2 antibody [EPR3278] (ab76473)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3278] to Anterior Gradient 2
- Suitable for: WB, IP, IHC-P, Flow Cyt (Intra), ICC/IF
- Reacts with: Human
Related conjugates and formulations
製品の概要
-
製品名
Anti-Anterior Gradient 2 antibody [EPR3278]
Anterior Gradient 2 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR3278] to Anterior Gradient 2 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IP, IHC-P, Flow Cyt (Intra), ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide within Human Anterior Gradient 2 aa 1-100. The exact sequence is proprietary.
-
ポジティブ･コントロール
- HT-29 and MCF-7 cell lysates. Human colon tissue. Human endometrium adenocarcinoma. ICC/IF: MCF7 cells Flow Cyt (intra): HT-29 cells IP: HT-29 whole cell lysate.
-
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR3278 -
アイソタイプ
IgG -
研究分野
関連製品
-
Alternative Versions
- Anti-Anterior Gradient 2 antibody [EPR3278] - BSA and Azide free (ab181829)
- Alexa Fluor® 488 Anti-Anterior Gradient 2 antibody [EPR3278] (ab199044)
- Alexa Fluor® 555 Anti-Anterior Gradient 2 antibody [EPR3278] (ab215293)
- Alexa Fluor® 568 Anti-Anterior Gradient 2 antibody [EPR3278] (ab312611)
- Alexa Fluor® 750 Anti-Anterior Gradient 2 antibody [EPR3278] (ab321729)
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab76473の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/5000 - 1/10000. Predicted molecular weight: 20 kDa.
|
|
| IP |
1/20.
|
|
| IHC-P | (1) |
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
| Flow Cyt (Intra) |
1/40.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| ICC/IF |
1/100.
|
| 特記事項 |
|---|
|
WB
1/5000 - 1/10000. Predicted molecular weight: 20 kDa. |
|
IP
1/20. |
|
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
|
Flow Cyt (Intra)
1/40. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
ICC/IF
1/100. |
ターゲット情報
-
機能
Required for MUC2 post-transcriptional synthesis and secretion. May play a role in the production of mucus by intestinal cells (By similarity). Proto-oncogene that may play a role in cell migration, cell differentiation and cell growth. -
組織特異性
Expressed strongly in trachea, lung, stomach, colon, prostate and small intestine. Expressed weakly in pituitary gland, salivary gland, mammary gland, bladder, appendix, ovary, fetal lung, uterus, pancreas, kidney, fetal kidney, testis, placenta, thyroid gland and in estrogen receptor (ER)-positive breast cancer cell lines. -
配列類似性
Belongs to the AGR family. -
細胞内局在
Secreted. Endoplasmic reticulum. - Information by UniProt
-
参照データベース
- Entrez Gene: 10551 Human
- Omim: 606358 Human
- SwissProt: O95994 Human
- Unigene: 530009 Human
-
別名
- AG 2 antibody
- AG 2 protein antibody
- AG-2 antibody
see all
画像
-
Western blot - Anti-Anterior Gradient 2 antibody [EPR3278] (ab76473)All lanes : Anti-Anterior Gradient 2 antibody [EPR3278] (ab76473) at 1/10000 dilution
Lane 1 : HT-29 cell lysates
Lane 2 : MCF-7 cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 20 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted? -
![Immunocytochemistry/ Immunofluorescence - Anti-Anterior Gradient 2 antibody [EPR3278] (ab76473)](https://www.abcam.co.jp/ps/products/76/ab76473/Images/ab76473-4-anti-anterior-gradient-2-antibody-epr3278-immunocytochemistry-mcf7-human.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Anterior Gradient 2 antibody [EPR3278] (ab76473)Immunocytochemistry/ Immunofluorescence analysis of MCF7 (human breast adenocarcinoma epithelial cell) cells labeling Anterior Gradient 2 with purified ab76473 at 1/100 dilution (3.5 µg/mL). Cells were fixed in 100% Methanol. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL) was used as the secondary antibody only control.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Anterior Gradient 2 antibody [EPR3278] (ab76473)](https://www.abcam.co.jp/ps/products/76/ab76473/Images/ab76473-91911-anti-anterior-gradient-2-antibody-epr3278-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Anterior Gradient 2 antibody [EPR3278] (ab76473)ab76473, at 1/500 dilution, staining Anterior Gradient 2 in paraffin-embedded human colon tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
![Immunoprecipitation - Anti-Anterior Gradient 2 antibody [EPR3278] (ab76473)](https://www.abcam.co.jp/ps/products/76/ab76473/Images/ab76473-7-anti-anterior-gradient-2-antibody-epr3278-immunoprecipitation-ht29-human.jpg)
Immunoprecipitation - Anti-Anterior Gradient 2 antibody [EPR3278] (ab76473)Purified ab76473 at 1/20 dilution (2µg) immunoprecipitating Anterior Gradient 2 in HT-29 whole cell lysate.
Lane 1 (input): HT-29 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate 10µg
Lane 2 (+): ab76473 + HT-29 whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab76473 in HT-29 whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 17 kDa -
![Flow Cytometry (Intracellular) - Anti-Anterior Gradient 2 antibody [EPR3278] (ab76473)](https://www.abcam.co.jp/ps/products/76/ab76473/Images/ab76473-6-anti-anterior-gradient-2-antibody-epr3278-flowcytometry-ht29-human.jpg)
Flow Cytometry (Intracellular) - Anti-Anterior Gradient 2 antibody [EPR3278] (ab76473)Intracellular Flow Cytometry analysis of HT-29 (Human colorectal adenocarcinoma epithelial cell) cells labeling Anterior Gradient 2 with purified ab76473 at 1/40 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cells without incubation with primary antibody and secondary antibody (Blue).
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Anterior Gradient 2 antibody [EPR3278] (ab76473)](https://www.abcam.co.jp/ps/products/76/ab76473/Images/ab76473-91910-anti-anterior-gradient-2-antibody-epr3278-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Anterior Gradient 2 antibody [EPR3278] (ab76473)ab76473, at 1/500 dilution, staining Anterior Gradient 2 in paraffin-embedded human endometrium adenocarcinoma by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
![Anti-Anterior Gradient 2 antibody [EPR3278] (ab76473)](https://www.abcam.co.jp/ps/products/76/ab76473/Images/ab76473-5-benefits-of-recombinant-antibodies.png)
Anti-Anterior Gradient 2 antibody [EPR3278] (ab76473)
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (21)
ab76473 は 21 報の論文で使用されています。
- Lu T et al. Bioinformatics analysis and single-cell RNA sequencing: elucidating the ubiquitination pathways and key enzymes in lung adenocarcinoma. J Thorac Dis 15:3885-3907 (2023). PubMed: 37559628
- Takada H et al. Single-cell transcriptomics uncovers EGFR signaling-mediated gastric progenitor cell differentiation in stomach homeostasis. Nat Commun 14:3750 (2023). PubMed: 37386010
- Wu D et al. Glutamine promotes O-GlcNAcylation of G6PD and inhibits AGR2 S-glutathionylation to maintain the intestinal mucus barrier in burned septic mice. Redox Biol 59:102581 (2023). PubMed: 36565645
- Zhou M et al. AGR2 and FOXA1 as prognostic markers in ER-positive breast cancer. BMC Cancer 23:743 (2023). PubMed: 37568077
- Ly TTG et al. Inhibitory Effect of Etravirine, a Non-Nucleoside Reverse Transcriptase Inhibitor, via Anterior Gradient Protein 2 Homolog Degradation against Ovarian Cancer Metastasis. Int J Mol Sci 23:N/A (2022). PubMed: 35055132