Anti-ANP32E 抗体 (ab5993)
Key features and details
- Rabbit polyclonal to ANP32E
- Suitable for: WB, ICC, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
製品の概要
-
製品名
Anti-ANP32E antibody -
製品の詳細
Rabbit polyclonal to ANP32E -
由来種
Rabbit -
特異性
This antibody does not cross-react with PHAPI or PHAP12a. -
アプリケーション
適用あり: WB, ICC, IHC-Pmore details -
種交差性
交差種: Rat, Human -
免疫原
Synthetic peptide corresponding to Human ANP32E (C terminal).
(Peptide available asab6242) -
ポジティブ･コントロール
- WB: A549, HepG2 and rat testis. IHC: Human small intestine tissue. ICC: Human small intestine cells.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at +4°C. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab5993の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 35 kDa.
|
|
| ICC |
Use a concentration of 10 µg/ml.
|
|
| IHC-P |
Use a concentration of 2 µg/ml.
|
| 特記事項 |
|---|
|
WB
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 35 kDa. |
|
ICC
Use a concentration of 10 µg/ml. |
|
IHC-P
Use a concentration of 2 µg/ml. |
ターゲット情報
-
機能
Inhibits activity of protein phosphatase 2A (PP2A). Does not inhibit protein phosphatase 1. May play a role in cerebellar development and synaptogenesis process by modulating PP2A activity. -
組織特異性
Expressed in peripheral blood leukocytes, colon, small intestine, prostate, thymus, spleen, skeletal muscle, liver and kidney. -
配列類似性
Belongs to the ANP32 family.
Contains 4 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain. -
翻訳後修飾
Phosphorylated. The phosphorylation is nuclear localization signal (NLS)-dependent. -
細胞内局在
Cytoplasm. Nucleus. - Information by UniProt
-
参照データベース
- Entrez Gene: 81611 Human
- Entrez Gene: 361999 Rat
- Omim: 609611 Human
- SwissProt: Q9BTT0 Human
- SwissProt: Q5XIE0 Rat
- Unigene: 656466 Human
- Unigene: 4268 Rat
-
別名
- Acidic (leucine rich) nuclear phosphoprotein 32 family member E antibody
- Acidic leucine-rich nuclear phosphoprotein 32 family member E antibody
- AN32E_HUMAN antibody
see all
画像
-
Western blot - Anti-ANP32E antibody (ab5993)
Western blot analysis of PHAP III expression in human A549 (A) and HepG2 (B) cells, and rat testis (C) with anti-PHAP III at 1 µg/ml. Western blot analysis of ANP32E expression in human A549 (A) and HepG2 (B) cells, and rat testis (C) with ab5993 at 1 µg/ml.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ANP32E antibody (ab5993)ab5993 at 2µg/ml staining ANP32E in human small intestine tissue by IHC
-
Immunocytochemistry - Anti-ANP32E antibody (ab5993)Immunofluorescence of PHAP III in Human Small Intestine cells using ab5993 at 20 ug/ml.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (9)
ab5993 は 9 報の論文で使用されています。
- Liu X et al. ANP32 Family as Diagnostic, Prognostic, and Therapeutic Biomarker Related to Immune Infiltrates in Hepatocellular Carcinoma. Dis Markers 2022:5791471 (2022). PubMed: 35280441
- Zhu X et al. ANP32E contributes to gastric cancer progression via NUF2 upregulation. Mol Med Rep 26:N/A (2022). PubMed: 35795988
- Wei J et al. Anp32e promotes renal interstitial fibrosis by upregulating the expression of fibrosis-related proteins. Int J Biol Sci 18:5897-5912 (2022). PubMed: 36263179
- Stefanelli G et al. The histone chaperone Anp32e regulates memory formation, transcription, and dendritic morphology by regulating steady-state H2A.Z binding in neurons. Cell Rep 36:109551 (2021). PubMed: 34407406
- Zhang J et al. Over-expression of ANP32E is associated with poor prognosis of pancreatic cancer and promotes cell proliferation and migration through regulating ß-catenin. BMC Cancer 20:1065 (2020). PubMed: 33148205
- Huang J et al. Up-regulated ANP32E promotes the thyroid carcinoma cell proliferation and migration via activating AKT/mTOR/HK2-mediated glycolysis. Gene 750:144681 (2020). PubMed: 32304784
- Choi S et al. Oxoglutarate dehydrogenase and acetyl-CoA acyltransferase 2 selectively associate with H2A.Z-occupied promoters and are required for histone modifications. Biochim Biophys Acta Gene Regul Mech 1862:194436 (2019). PubMed: 31682939
- Shin H et al. Transcriptional regulation mediated by H2A.Z via ANP32e-dependent inhibition of protein phosphatase 2A. Biochim Biophys Acta 1861:481-496 (2018). ChIP-sequencing ; Mouse . PubMed: 29524612
- Mao Z et al. Anp32e, a higher eukaryotic histone chaperone directs preferential recognition for H2A.Z. Cell Res 24:389-99 (2014). Human . PubMed: 24613878