Name: Anti-Annexin V/ANXA5 antibody
SKU: ab14196
Rating: 4 (19 reviews)

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Western blot - Anti-Annexin V/ANXA5 antibody (ab14196)

Key features and details

Rabbit polyclonal to Annexin V/ANXA5
Suitable for: WB
Reacts with: Human
Isotype: IgG

リコンビナント抗体で、ロット間での高い再現性を実現

Anti-Annexin V/ANXA5 antibody [EPR3979] (ab108321)

異なるロット間での安定した再現性
容易なスケールアップ
評価試験による特異性の確認済み
倫理基準に準拠 - アニマル・フリーの生産

製品名

Anti-Annexin V/ANXA5 antibody
Annexin V/ANXA5 一次抗体製品一覧
製品の詳細

Rabbit polyclonal to Annexin V/ANXA5
由来種

Rabbit
アプリケーション

適用あり: WBmore details
種交差性

交差種: Human
免疫原

Recombinant full length protein corresponding to Human Annexin V/ANXA5.
Database link: P08758
(Peptide available as ab52111)
特記事項

This product is manufactured by BioVision, an Abcam company and was previously called 3357 Annexin V Antibody.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の状態

Liquid
保存方法

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
バッファー

Preservative: 0.03% Proclin 300
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 0.5% BSA, 0.015% EDTA
Concentration information loading...
精製度

Immunogen affinity purified
ポリ/モノ

ポリクローナル
アイソタイプ

IgG
研究分野

The Abpromise guarantee

Abpromise保証は、次のテスト済みアプリケーションにおけるab14196の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション	Abreviews	特記事項
WB	(8)	Use a concentration of 1 - 4 µg/ml.

特記事項
WB Use a concentration of 1 - 4 µg/ml.

機能

This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.
関連疾患

Pregnancy loss, recurrent, 3
配列類似性

Belongs to the annexin family.
Contains 4 annexin repeats.
ドメイン

The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.
A pair of annexin repeats may form one binding site for calcium and phospholipid.
翻訳後修飾

S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.
Target information above from: UniProt accession P08758 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
参照データベース
- Entrez Gene: 308 Human
- Omim: 131230 Human
- SwissProt: P08758 Human
- Unigene: 480653 Human
別名
- Anchorin CII antibody
- Annexin 5 antibody
- Annexin A5 antibody
- Annexin V antibody
- Annexin-5 antibody
- Annexin5 antibody
- AnnexinA5 antibody
- AnnexinV antibody
- ANX 5 antibody
- ANX A5 antibody
- ANX5 antibody
- ANXA5 antibody
- ANXA5_HUMAN antibody
- Calphobindin I antibody
- CBP-I antibody
- Endonexin II antibody
- ENX 2 antibody
- ENX2 antibody
- Lipocortin V antibody
- PAP I antibody
- PAP-I antibody
- Placental anticoagulant protein 4 antibody
- Placental anticoagulant protein I antibody
- PLACENTAL PROTEIN 4 antibody
- PP 4 antibody
- Pp4 antibody
- RPRGL3 antibody
- Thromboplastin inhibitor antibody
- VAC-alpha antibody
- Vascular anticoagulant-alpha antibody
see all

Western blot - Anti-Annexin V/ANXA5 antibody (ab14196)

Anti-Annexin V/ANXA5 antibody (ab14196) + HeLa cell lysate

Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

ab14196 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab14196 は 79 報の論文で使用されています。

Marsilio S et al. Characterization of the intestinal mucosal proteome in cats with inflammatory bowel disease and alimentary small cell lymphoma. J Vet Intern Med 35:179-189 (2021). PubMed: 33471936
Feige P et al. Analysis of human satellite cell dynamics on cultured adult skeletal muscle myofibers. Skelet Muscle 11:1 (2021). PubMed: 33397479
Haeckel A et al. Long-circulating XTEN864-annexin A5 fusion protein for phosphatidylserine-related therapeutic applications. Apoptosis 26:534-547 (2021). PubMed: 34405304
Robinson ER et al. Minicircles for a two-step blood biomarker and PET imaging early cancer detection strategy. J Control Release 335:281-289 (2021). PubMed: 34029631
Tripathi BK et al. Inhibition of cytoplasmic EZH2 induces antitumor activity through stabilization of the DLC1 tumor suppressor protein. Nat Commun 12:6941 (2021). PubMed: 34862367

View all Publications for this product

レビューと Q&A

Show All レビュー Q&A

レビューを送る質問を送る

1-10 of 24 Abreviews or Q&A

Application

Flow Cytometry

Sample

Human Cell (Jurkat cells)

Permeabilization

Gating Strategy

no gate

Specification

Jurkat cells

Preparation

Cell harvesting/tissue preparation method: cell culture
Sample buffer: Binding buffer

Fixation

none

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Feb 06 2018

Application

Immunocytochemistry/ Immunofluorescence

Sample

Human Cell (Jurkat cells)

Permeabilization

Specification

Jurkat cells

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Fixative

Formaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Feb 06 2018

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Human Tissue sections (skin)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: citrat buffer ph6,0

Permeabilization

Yes - 0,2% Triton/PBS

Specification

skin

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Feb 06 2018

Application

Immunohistochemistry (Frozen sections)

Sample

Jaculus jaculus Tissue sections (Skin)

Permeabilization

Yes - 0.1% Triton

Specification

Skin

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Mai Tran

Verified customer

投稿 Nov 04 2016

Application

Western blot

Sample

Human Cell lysate - whole cell (huamn)

Gel Running Conditions

Reduced Denaturing

Loading amount

50 µg

Treatment

alpha synuclein

Specification

huamn

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Ms. Sehee Oh

Verified customer

投稿 Jul 13 2016

Application

Western blot

Sample

Human Cell lysate - whole cell (HBMEC (Human Brain Microvascular Endothelial Cell))

Gel Running Conditions

Reduced Denaturing (10)

Loading amount

20 µg

Specification

HBMEC (Human Brain Microvascular Endothelial Cell)

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Dec 31 2015

Application

Western blot

Sample

Rat Cell lysate - whole cell (Pericytes)

Gel Running Conditions

Reduced Denaturing (10%)

Loading amount

20 µg

Specification

Pericytes

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Dec 28 2015

Application

Western blot

Sample

Mouse Cell lysate - whole cell (bEnd.3)

Gel Running Conditions

Reduced Denaturing (10% gel)

Loading amount

20 µg

Specification

bEnd.3

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Dec 07 2015

Application

Immunohistochemistry (PFA perfusion fixed frozen sections)

Sample

Mouse Tissue sections (Embryonic 15 day brain)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Citrate buffer, pH 6

Permeabilization

Yes - 0.5% Triton X-100 in PBS

Specification

Embryonic 15 day brain

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 May 23 2014

Application

Immunohistochemistry (Frozen sections)

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C

Sample

Mouse Tissue sections (lumbar spinal cord, 25 micron)

Specification

lumbar spinal cord, 25 micron

Permeabilization

Yes - 0.5% Triton X100

Fixative

Formaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Apr 04 2014

1-10 of 24 Abreviews or Q&A

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Key features and details

製品の概要

製品名

製品の詳細

由来種

アプリケーション

種交差性

免疫原

特記事項

製品の特性

製品の状態

保存方法

バッファー

精製度

ポリ/モノ

アイソタイプ

研究分野

関連製品

Compatible Secondaries

Isotype control

Recombinant Protein

Related Products

アプリケーション

The Abpromise guarantee

ターゲット情報

機能

関連疾患

配列類似性

ドメイン

翻訳後修飾

参照データベース

別名

画像

プロトコール

データシートおよび資料

SDS download

Datasheet download

参考文献 (79)

レビューと Q&A

Flow Cytometry abreview for Anti-Annexin V/ANXA5 antibody

Immunocytochemistry/ Immunofluorescence abreview for Anti-Annexin V antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Annexin V/ANXA5 antibody

Immunohistochemistry (Frozen sections) abreview for Anti-Annexin V antibody

Western blot abreview for Anti-Annexin V/ANXA5 antibody

Western blot abreview for Anti-Annexin V antibody

Western blot abreview for Anti-Annexin V/ANXA5 antibody

Western blot abreview for Anti-Annexin V antibody

Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Annexin V/ANXA5 antibody

Immunohistochemistry (Frozen sections) abreview for Anti-Annexin V antibody