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  1. Link

    products/primary-antibodies/annexin-vanxa5-antibody-1f4-1a5-ab54775.pdf

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Cell Biology Apoptosis Annexin V Reagents
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Anti-Annexin V/ANXA5 抗体 [1F4-1A5] (ab54775)

  • Datasheet
Reviews (7)Q&A (8)References (22)

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Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Western blot - Anti-Annexin V/ANXA5 antibody [1F4-1A5] (ab54775)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin V/ANXA5 antibody [1F4-1A5] (ab54775)
  • Immunocytochemistry/ Immunofluorescence - Anti-Annexin V/ANXA5 antibody [1F4-1A5] (ab54775)
  • Flow Cytometry - Anti-Annexin V/ANXA5 antibody [1F4-1A5] (ab54775)
  • Immunoprecipitation - Anti-Annexin V/ANXA5 antibody [1F4-1A5] (ab54775)
  • Western blot - Anti-Annexin V/ANXA5 antibody [1F4-1A5] (ab54775)
  • Immunocytochemistry/ Immunofluorescence - Anti-Annexin V/ANXA5 antibody [1F4-1A5] (ab54775)

Key features and details

  • Mouse monoclonal [1F4-1A5] to Annexin V/ANXA5
  • Suitable for: IP, WB, ICC/IF, IHC-P, Flow Cyt
  • Reacts with: Human
  • Isotype: IgG1

こちらの製品もご検討ください

アッセイ
Product image
Annexin V-FITC Apoptosis Staining / Detection Kit (ab14085)
タンパク質
Product image
Recombinant Human Annexin V/ANXA5 protein (His tag) (ab157342)
一次抗体
Product image
Anti-Calnexin antibody - ER Marker (ab22595)

関連製品

製品の概要

  • 製品名

    Anti-Annexin V/ANXA5 antibody [1F4-1A5]
    Annexin V/ANXA5 一次抗体 製品一覧
  • 製品の詳細

    Mouse monoclonal [1F4-1A5] to Annexin V/ANXA5
  • 由来種

    Mouse
  • アプリケーション

    適用あり: IP, WB, ICC/IF, IHC-P, Flow Cytmore details
  • 種交差性

    交差種: Human
  • 免疫原

    Recombinant full length protein corresponding to Human Annexin V/ANXA5 aa 1-321.

  • 特記事項

    This product was changed from ascites to tissue culture supernatant on 22/03/2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー

    pH: 7.40
    Constituent: PBS
  • Concentration information loading...
  • 精製度

    Tissue culture supernatant
  • ポリ/モノ

    モノクローナル
  • クローン名

    1F4-1A5
  • アイソタイプ

    IgG1
  • 軽鎖の種類

    kappa
  • 研究分野

    • Cell Biology
    • Apoptosis
    • Annexin V Reagents
    • Cancer
    • Invasion/microenvironment
    • Apoptosis
    • Detection kits
    • Annexin V
    • Cardiovascular
    • Atherosclerosis
    • Apoptosis
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Apoptosis marker and proteins ELISA kits
    • Cancer
    • Cell Death
    • Apoptosis
    • Apoptosis Markers
    • Annexin V
    • Annexin V Detection Kits
    • Cancer
    • Cell Death
    • Apoptosis
    • Apoptosis Markers
    • Annexin V
    • Annexin V Reagents

関連製品

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)
  • Recombinant Protein

    • Recombinant Human Annexin V/ANXA5 protein (His tag) (ab157342)
  • Related Products

    • Pentoxifylline, Phosphodiesterase and cytokine inhibitor (ab120725)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab54775の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IP
Use at an assay dependent concentration.
WB (6)
Use at an assay dependent concentration. Predicted molecular weight: 36 kDa.
ICC/IF
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
Flow Cyt
Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

特記事項
IP
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 36 kDa.
ICC/IF
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
Flow Cyt
Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能

    This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.
  • 関連疾患

    Pregnancy loss, recurrent, 3
  • 配列類似性

    Belongs to the annexin family.
    Contains 4 annexin repeats.
  • ドメイン

    The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.
    A pair of annexin repeats may form one binding site for calcium and phospholipid.
  • 翻訳後修飾

    S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.
  • Target information above from: UniProt accession P08758 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 308 Human
    • Omim: 131230 Human
    • SwissProt: P08758 Human
    • Unigene: 480653 Human
    • 別名

      • Anchorin CII antibody
      • Annexin 5 antibody
      • Annexin A5 antibody
      • Annexin V antibody
      • Annexin-5 antibody
      • Annexin5 antibody
      • AnnexinA5 antibody
      • AnnexinV antibody
      • ANX 5 antibody
      • ANX A5 antibody
      • ANX5 antibody
      • ANXA5 antibody
      • ANXA5_HUMAN antibody
      • Calphobindin I antibody
      • CBP-I antibody
      • Endonexin II antibody
      • ENX 2 antibody
      • ENX2 antibody
      • Lipocortin V antibody
      • PAP I antibody
      • PAP-I antibody
      • Placental anticoagulant protein 4 antibody
      • Placental anticoagulant protein I antibody
      • PLACENTAL PROTEIN 4 antibody
      • PP 4 antibody
      • Pp4 antibody
      • RPRGL3 antibody
      • Thromboplastin inhibitor antibody
      • VAC-alpha antibody
      • Vascular anticoagulant-alpha antibody
      see all

    画像

    • Western blot - Anti-Annexin V/ANXA5 antibody [1F4-1A5] (ab54775)
      Western blot - Anti-Annexin V/ANXA5 antibody [1F4-1A5] (ab54775)

      ab54775 Western Blot analysis of ANXA5/ANXA5 expression in Hela cells.

      This image was generated using the ascites version of the product.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin V/ANXA5 antibody [1F4-1A5] (ab54775)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin V/ANXA5 antibody [1F4-1A5] (ab54775)

      Annexin V/ANXA5 antibody (ab54775) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human colon.

      This image was generated using the ascites version of the product.

    • Immunocytochemistry/ Immunofluorescence - Anti-Annexin V/ANXA5 antibody [1F4-1A5] (ab54775)
      Immunocytochemistry/ Immunofluorescence - Anti-Annexin V/ANXA5 antibody [1F4-1A5] (ab54775)

      ab54775 at 10 ug/ml staining Annexin V/ANXA5 in human Hela cells by Immunocytochemistry / Immunofluorescence.

      This image was generated using the ascites version of the product.

    • Flow Cytometry - Anti-Annexin V/ANXA5 antibody [1F4-1A5] (ab54775)
      Flow Cytometry - Anti-Annexin V/ANXA5 antibody [1F4-1A5] (ab54775)

      Overlay histogram showing HeLa cells stained with ab54775 (red line). The cells were fixed with 100% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54775, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

      This image was generated using the ascites version of the product.

    • Immunoprecipitation - Anti-Annexin V/ANXA5 antibody [1F4-1A5] (ab54775)
      Immunoprecipitation - Anti-Annexin V/ANXA5 antibody [1F4-1A5] (ab54775)

      Immunoprecipitation of ANXA5 transfected lysate using ab54775 monoclonal antibody and Protein A Magnetic Bead and immunoblotted with a ANXA5 rabbit polyclonal antibody.

      This image was generated using the ascites version of the product.

    • Western blot - Anti-Annexin V/ANXA5 antibody [1F4-1A5] (ab54775)
      Western blot - Anti-Annexin V/ANXA5 antibody [1F4-1A5] (ab54775)

      Western Blot analysis of ANXA5 expression in transfected 293T cell line by ab54775.
      Lane 1: ANXA5 transfected lysate(35.9 KDa).
      Lane 2: Non-transfected lysate.

      This image was generated using the ascites version of the product.

    • Immunocytochemistry/ Immunofluorescence - Anti-Annexin V/ANXA5 antibody [1F4-1A5] (ab54775)
      Immunocytochemistry/ Immunofluorescence - Anti-Annexin V/ANXA5 antibody [1F4-1A5] (ab54775)

      ab54775 staining Annexin V/ANXA5 in HeLa cells treated with pentoxifylline (ab120725), by ICC/IF. Increase of Annexin V/ANXA5 correlates with increased concentration of pentoxifylline, as described in literature.
      The cells were incubated at 37°C for 24h in media containing different concentrations of ab120725 (pentoxifylline) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab54775 (10 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

      This image was generated using the ascites version of the product.

    プロトコール

    • Flow cytometry protocols
    • Immunoprecipitation protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    データシートおよび資料

    • Datasheet download

      Download

    参考文献 (22)

    ab54775 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab54775 は 22 報の論文で使用されています。

    • Rassol N  et al. Co-administration with A1M does not influence apoptotic response of 177Lu-octreotate in GOT1 neuroendocrine tumors. Sci Rep 13:6417 (2023). PubMed: 37076494
    • Zietzer A  et al. Activation of neutral sphingomyelinase 2 through hyperglycemia contributes to endothelial apoptosis via vesicle-bound intercellular transfer of ceramides. Cell Mol Life Sci 79:48 (2021). PubMed: 34951654
    • Martínez-Lagunas K  et al. In vivo detection of programmed cell death during mouse heart development. Cell Death Differ 27:1398-1414 (2020). PubMed: 31570857
    • Zietzer A  et al. The RNA-binding protein hnRNPU regulates the sorting of microRNA-30c-5p into large extracellular vesicles. J Extracell Vesicles 9:1786967 (2020). PubMed: 32944175
    • Bartolome F  et al. Annexin A5 prevents amyloid-ß-induced toxicity in choroid plexus: implication for Alzheimer's disease. Sci Rep 10:9391 (2020). PubMed: 32523019
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-10 of 15 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Annexin V/ANXA5 antibody

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (Rat brain)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris-EDTA pH 9
    Permeabilization
    No
    Specification
    Rat brain
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
    Fixative
    Parafin
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Jan 23 2018

    Western blot abreview for Anti-Annexin V antibody

    Average
    Abreviews
    Abreviews
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (human)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    50 µg
    Treatment
    alpha synuclein
    Specification
    human
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MS. Sehee Oh

    Verified customer

    投稿 Jul 13 2016

    Western blot abreview for Anti-Annexin V/ANXA5 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Tissue lysate - whole (Human cortex lysate)
    Gel Running Conditions
    Reduced Denaturing (10% gel)
    Loading amount
    20 µg
    Specification
    Human cortex lysate
    Blocking step
    Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. XXXXXX XXXXXX

    Verified customer

    投稿 Oct 18 2012

    Western blot abreview for Anti-Annexin V antibody

    Inconclusive
    Abreviews
    Abreviews
    Application
    Western blot
    Sample
    Rat Cell lysate - whole cell (cultured epithelial cells)
    Loading amount
    30 µg
    Specification
    cultured epithelial cells
    Gel Running Conditions
    Reduced Denaturing (10% gel)
    Blocking step
    Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Oct 16 2012

    Western blot abreview for Anti-Annexin V/ANXA5 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (U2OS)
    Gel Running Conditions
    Non-reduced Denaturing
    Loading amount
    25 µg
    Specification
    U2OS
    Blocking step
    Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Sep 25 2012

    Western blot abreview for Anti-Annexin V antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (smooth muscle cells)
    Loading amount
    25 µg
    Specification
    smooth muscle cells
    Gel Running Conditions
    Reduced Denaturing (10%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Mar 23 2012

    Question

    I am looking for an anti-annexin V antibody for IHC staining in section of porcine small intestine. Can you tell me whether the above-mentioned antibody is cross-reactive with pigs?

    Read More

    Abcam community

    Verified customer

    Asked on Jan 09 2013

    Answer

    Vielen Dank für Ihren Anruf und für Ihr Interesse an unseren Produkten.

    Unseres Wissens nach wurde ab54775 Anti-Annexin V antibody bisher noch nicht am Schwein getestet. Falls Sie dies selbst testen möchten, kann ich Ihnen zur Zeit ein spezielles Angebot über einen 100%igen Abreview-Rabatt anbieten. Bei diesem Angebot bekommen Sie einen Rabatt für eine zukünftige Bestellung, wenn Sie uns ein Abreview mit dem Testresultat zusenden. Der Rabatt würde den ganzen Wert von ab54775 abdecken, und gegen eine erneute Bestellung von uns verrechnet werden.

    Um von diesem Angebot profitieren zu können, folgen Sie bitte diesen Schritten:

    1.) Bestätigen Sie mir bitte, dass Sie ab54775 in Schwein testen möchten.

    2.) Bitte bestellen Sie erst nach Erhalt des Rabattcodes.

    3.) Bestellen Sie den Antikörper wie üblich per Telefon, Email oder Fax

    4.) Testen Sie den Antikörper in Schwein.
    5.) Teilen Sie uns das Ergebnis Ihres Tests durch ein Abreview mit und notieren Sie den Discount Code in dem Feld "additional notes"
    Unter der folgenden URL können Sie mehr über unser Abreview System erfahren:
    https://www.abcam.com/abreviews

    6.) Der Rabattcode ist nach dem Abschicken des Abreviews aktiv. Bitte beachten Sie, dass der Rabattcode innerhalb von 4 Monaten nach Ausstellung eingelöst werden muss.

    Der Rabattcode wird gültig unabhängig davon, ob Ihr Ergebnis positiv oder negativ ist.

    Die Bedingungen zu unserem 100% Abreview Rabatt können Sie unter dem folgenden Link nachlesen:
    https://www.abcam.com/collaborationdiscount

    Ich hoffe, diese Informationen helfen Ihnen weiter. Falls Sie einen Rabattcode erhalten möchten oder weitere Fragen haben, zögern Sie bitte nicht, sich wieder an mich zu wenden.

    Read More

    Abcam Scientific Support

    Answered on Jan 09 2013

    Question

    Thank you for your reply.
    Out of the antibodies you have suggested, which one would work the best in human tissue? - which one has the least cross-reactivity with human?

    Regards

    Read More

    Abcam community

    Verified customer

    Asked on Feb 22 2012

    Answer

    Thank you for your inquiry.

    These antibodies have not been compared experimentally by us. They all should be suitable for your purposes.

    I personally would choose ab98693. It only detects the IgG1 heavy chain from mouse and additionally has been pre-absorbed against rat and human IgG to ensure specificity.

    https://www.abcam.com/Goat-Mouse-IgG1-HRP-preadsorbed-ab98693.html (or use the following: https://www.abcam.com/Goat-Mouse-IgG1-HRP-preadsorbed-ab98693.html).

    I hope this information is helpful in your decision with antibody to use for your future experiments.

    Read More

    Abcam Scientific Support

    Answered on Feb 22 2012

    Question

    Hello

    I have recently contacted you regarding an Annexin V antibody. You have suggested that the extra bands we are getting in our blots might be a result of posttranslational modifications such as phosphorylation.

    in some recent discussions with our colleagues, one has suggested that the band may be due to inspecific binding of our secondary anti mouse HRP antibody to the IgG light chain which is abundant in plasma and which weighs 25 kDa (which is exactly where we see this band). we have confirmed that there is inspecific binding at this molecular weight even when no primary antibody is present. We were advised to use a different anti mouse antibody, preferably one which binds to the heavy chain, which might result in inspecific binding but the band should be higher up and thus not interfeering with our Anexin band (which at the moment gets quite a lot of interfearance from the 25 kDa band which is very prominent, even though our plasma samples are from humans).

    I would like to enquire whether you have any anti mouse antibodies which bind to the IgG heavy chain and if yes, would you be willing to send us a sample so that we can try this method and see if its solves our Annexin V problem?

    thank you very much

    Read More

    Abcam community

    Verified customer

    Asked on Feb 21 2012

    Answer

    Thank you for contacting us. Because we carry over 70,000 products, it isn't feasible for us to keep small sample sizes of our products.

    We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase.

    We do offer three anti mouse IgG antibodies that bind the Fc part of the primary antibody:

    ab98717
    https://www.abcam.com/Goat-Mouse-IgG-Fc-HRP-preadsorbed-ab98717.html (or use the following: https://www.abcam.com/Goat-Mouse-IgG-Fc-HRP-preadsorbed-ab98717.html).

    ab98741
    https://www.abcam.com/Goat-Fab2-Mouse-IgG-Fc-HRP-pre-adsorbed-ab98741.html (or use the following: https://www.abcam.com/Goat-Fab2-Mouse-IgG-Fc-HRP-pre-adsorbed-ab98741.html).

    ab5879
    https://www.abcam.com/Goat-Fab2-Mouse-IgG-Fc-HRP-pre-adsorbed-ab5879.html (or use the following: https://www.abcam.com/Goat-Fab2-Mouse-IgG-Fc-HRP-pre-adsorbed-ab5879.html).

    We also offer two antibodies directed at the IgG1 mouse heavy chain:

    ab97240
    https://www.abcam.com/Goat-Mouse-IgG1-HRP-ab97240.html (or use the following: https://www.abcam.com/Goat-Mouse-IgG1-HRP-ab97240.html).

    ab98693
    https://www.abcam.com/Goat-Mouse-IgG1-HRP-preadsorbed-ab98693.html (or use the following: https://www.abcam.com/Goat-Mouse-IgG1-HRP-preadsorbed-ab98693.html).


    I hope this information is helpful to find an antibody suitable for your purposes. Please do not hesitate to contact us again with any other questions.

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    Abcam Scientific Support

    Answered on Feb 21 2012

    Question

    Currently, I am using the abcam Annexin V antibody (ab54775) and I would like to know whether this antibody is capable to block the Annexin V (e.g. on cell surfaces). If yes, I would be interested to get some references if possibleI. If not, could you offer me a product to block Annexin V (either a blocking AV antibody or maybe a soluble phosphatidylserine).

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    Abcam community

    Verified customer

    Asked on Feb 07 2012

    Answer

    Thank you for contacting us and for your patience.

    I have contacted the originator of anti-Annexin V antibody ab54775 and our other anti-Annexin V antibodies:

    Unfortunately, it is not known whether ab54775 is capable of blocking Annexin V as this is not normally tested. Generally, it is testedif the antibody binding can be blocked, for example by using the immunogen - in this caserecombinant protein could be used to block antibody. However, we have no experience in using recombinant protein or other materials to block this antibody.
    As the expression system of the recombinant protein was cell-free protein synthesis system (in vitro wheat germ protein expression system). Glycosylation and other natural eukaryotic modifications of synthesized protein do not take place in this cell-free system. Also, we are not sure whether recombinant protein will be folded correctly.

    In general, we do not know whether any of our antibodies could block the Annexin V binding. The antibodies are specific for different domains of human Annexin V (for example ab108194 amino acids 296 - 320 which is in the fourth Annexin domain, http://www.uniprot.org/uniprot/P08758). If the binding to these domainsis sufficient to block Annexin V though, has not been determined yet.

    Also, we currently do not have other products such as soluble phosphatidylserine in our catalogue which might be suitable for your experiments.

    We are happy to help customers find the most suitable products for their research purposes; however we are not specialized in this particular field and do not want to recommend a product that may be unsuitable. We would encourage you to consult the latest literature available through PubMed and other resources in order to find the most up-to-date information about this specific research interests.

    Should you decide to go ahead and test one of ourAnnexin V antibodies in blocking experiments, we encourage you to submit an Abreview of your results. We appreciate all customer feedback, whether positive or negative, and we make all product information available to researchers. To find out more about our Abreview system, please see the following webpage: https://www.abcam.com/abreviews

    I am sorry that I could not be more helpful, but I hope that the available literature in this area can provide some clarification. Please do not hesitate to contact us again with other needs or with any questions about our products.

    Read More

    Abcam Scientific Support

    Answered on Feb 07 2012

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