Anti-Annexin-6/ANXA6 抗体 (ab31026)
Key features and details
- Rabbit polyclonal to Annexin-6/ANXA6
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Annexin-6/ANXA6 antibody
Annexin-6/ANXA6 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Annexin-6/ANXA6 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Synthetic peptide within Human Annexin-6/ANXA6 aa 1-100. The exact sequence is proprietary.
Database link: P08133 -
ポジティブ･コントロール
- IHC-P: Human L8 cells. Liver.
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特記事項
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab31026の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (3) |
Use at an assay dependent concentration. Detects a band of approximately 76 kDa (predicted molecular weight: 76 kDa).
|
| IHC-P |
1/50.
1/50 for 30 min at room temperature. Requires boiling of tissue sections in 10mM citrate buffer, pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes. |
| 特記事項 |
|---|
|
WB
Use at an assay dependent concentration. Detects a band of approximately 76 kDa (predicted molecular weight: 76 kDa). |
|
IHC-P
1/50. 1/50 for 30 min at room temperature. Requires boiling of tissue sections in 10mM citrate buffer, pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes. |
ターゲット情報
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機能
May associate with CD21. May regulate the release of Ca(2+) from intracellular stores. -
配列類似性
Belongs to the annexin family.
Contains 8 annexin repeats. -
ドメイン
A pair of annexin repeats may form one binding site for calcium and phospholipid. -
翻訳後修飾
Phosphorylated in response to growth factor stimulation. -
細胞内局在
Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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参照データベース
- Entrez Gene: 309 Human
- Entrez Gene: 11749 Mouse
- Entrez Gene: 79125 Rat
- Omim: 114070 Human
- SwissProt: P08133 Human
- SwissProt: P14824 Mouse
- SwissProt: P48037 Rat
- Unigene: 412117 Human
see all -
別名
- 67 kDa calelectrin antibody
- Annexin A6 antibody
- Annexin VI antibody
see all
画像
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Western blot - Anti-Annexin-6/ANXA6 antibody (ab31026)This image is courtesy of an anonymous AbreviewAnti-Annexin-6/ANXA6 antibody (ab31026) at 1/500 dilution + Mouse heart tissue lysate at 50 µg
Secondary
HRP-conjugated Goat anti-rabbit IgG at 1/7500 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 76 kDa
Observed band size: 76 kDa
Exposure time: 5 seconds
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin-6/ANXA6 antibody (ab31026)Ab31026, at a dilution of 1/50, staining formalin fixed paraffin embedded Annexin-6/ANXA6 in human liver tissue by Immunohistochemistry.
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Western blot - Anti-Annexin-6/ANXA6 antibody (ab31026)All lanes : Anti-Annexin-6/ANXA6 antibody (ab31026) at 1 µg/ml
Lane 1 : Human placenta tissue lysate - total protein (ab29745)
Lane 2 : Human kidney tissue lysate - total protein (ab30203)
Lane 3 : JEG-3 (Human placental choriocarcinoma cell line) Whole Cell Lysate
Lane 4 : A498 (Human Kidney Carcinoma) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 76 kDa
Observed band size: 76 kDa
Additional bands at: 37 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutes -
Western blot - Anti-Annexin-6/ANXA6 antibody (ab31026)Anti-Annexin-6/ANXA6 antibody (ab31026) at 1/25 dilution + lysate prepared from 293 cells
Predicted band size: 76 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (18)
ab31026 は 18 報の論文で使用されています。
- Whitehead M et al. Vascular smooth muscle cell senescence accelerates medin aggregation via small extracellular vesicle secretion and extracellular matrix reorganization. Aging Cell 22:e13746 (2023). PubMed: 36433666
- Tejwani L et al. Reduction of nemo-like kinase increases lysosome biogenesis and ameliorates TDP-43-related neurodegeneration. J Clin Invest 133:N/A (2023). PubMed: 37384409
- Sun X et al. ANXA6 suppresses the tumorigenesis of cervical cancer through autophagy induction. Clin Transl Med 10:e208 (2020). PubMed: 33135350
- Demonbreun AR et al. Recombinant annexin A6 promotes membrane repair and protects against muscle injury. J Clin Invest 129:4657-4670 (2019). PubMed: 31545299
- Raouf R et al. Inhibition of somatosensory mechanotransduction by annexin A6. Sci Signal 11:N/A (2018). PubMed: 29921656