Anti-Annexin-4/ANXA4 抗体 [EPR22929-208]

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin-4/ANXA4 antibody [EPR22929-208] (AB256456)

Immunohistochemical analysis of paraffin-embedded human endometrium tissue labeling Annexin-4/ANXA4 with ab256456 at 1/5000 dilution (0.103 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in human endometrium (PMID : 16954445) is observed. The section was incubated with ab256456 for 10 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin-4/ANXA4 antibody [EPR22929-208] (AB256456)

Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling Annexin-4/ANXA4 with ab256456 at 1/5000 dilution (0.103 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in human kidney (PMID : 9720986) is observed. The section was incubated with ab256456 for 10 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin-4/ANXA4 antibody [EPR22929-208] (AB256456)

Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling Annexin-4/ANXA4 with ab256456 at 1/5000 dilution (0.103 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in centroacinar cells and islets of human pancreas. The section was incubated with ab256456 for 10 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

• Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Annexin-4/ANXA4 antibody [EPR22929-208] (AB256456)

Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HepG2 (human hepatocellular carcinoma epithelial cell) cells labelling Annexin-4/ANXA4 with ab256456 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Annexin-4/ANXA4 antibody [EPR22929-208] (AB256456)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HepG2 (human hepatocellular carcinoma epithelial cell) cells labeling Annexin-4/ANXA4 with ab256456 at 1/100 5.2 μg/ml dilution, followed by ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary antibody at 1/1000 2 μg/ml dilution (Green). Confocal image showing nuclear, cytoplasmic and staining in HepG2 cell line. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at 1/1000 2 μg/ml dilution.

• IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-Annexin-4/ANXA4 antibody [EPR22929-208] (AB256456)

Immunohistochemical analysis of frozen section of 4% PFA-fixed, 0.2% Triton X-100 permeabilized rat kidney tissue labeling Annexin-4/ANXA4 with ab256456 at 1/100 dilution, followed by ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at 1/1000 dilution (green). Positive staining on rat kidney (PMID : 15145526) is observed. The nuclear counter stain is DAPI (blue).
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at 1/1000 dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

• Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Annexin-4/ANXA4 antibody [EPR22929-208] (AB256456)

Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling Annexin-4/ANXA4 with ab256456 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

• IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-Annexin-4/ANXA4 antibody [EPR22929-208] (AB256456)

Immunohistochemical analysis of frozen section of 4% PFA-fixed, 0.2% Triton X-100 permeabilized mouse pancreas tissue labeling Annexin-4/ANXA4 with ab256456 at 1/100 dilution, followed by ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at 1/1000 dilution (green). Positive staining on mouse pancreas (PMID : 25176043) is observed. The nuclear counter stain is DAPI (blue).
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at 1/1000 dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin-4/ANXA4 antibody [EPR22929-208] (AB256456)

Immunohistochemical analysis of paraffin-embedded rat stomach tissue labeling Annexin-4/ANXA4 with ab256456 at 1/5000 dilution (0.103 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in rat stomach. The section was incubated with ab256456 for 10 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin-4/ANXA4 antibody [EPR22929-208] (AB256456)

Immunohistochemical analysis of paraffin-embedded mouse stomach tissue labeling Annexin-4/ANXA4 with ab256456 at 1/5000 dilution (0.103 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in mouse stomach. The section was incubated with ab256456 for 10 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

• IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-Annexin-4/ANXA4 antibody [EPR22929-208] (AB256456)

Immunohistochemical analysis of frozen section of 4% PFA-fixed, 0.2% Triton X-100 permeabilized mouse kidney tissue labeling Annexin-4/ANXA4 with ab256456 at 1/100 dilution, followed by ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at 1/1000 dilution (green). Positive staining on mouse kidney (PMID : 15145526) is observed. The nuclear counter stain is DAPI (blue).
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at 1/1000 dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Annexin-4/ANXA4 antibody [EPR22929-208] (AB256456)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labeling Annexin-4/ANXA4 with ab256456 at 1/100 5.2 μg/ml dilution, followed by ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary antibody at 1/1000 2 μg/ml dilution (Green). Confocal image showing nuclear, cytoplasmic and staining in NIH/3T3 cell line. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at 1/1000 2 μg/ml dilution.

• IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-Annexin-4/ANXA4 antibody [EPR22929-208] (AB256456)

Immunohistochemical analysis of frozen section of 4% PFA-fixed, 0.2% Triton X-100 permeabilized rat pancreas tissue labeling Annexin-4/ANXA4 with ab256456 at 1/100 dilution, followed by ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at 1/1000 dilution (green). Positive staining on mouse pancreas (PMID : 25176043) is observed. The nuclear counter stain is DAPI (blue).
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at 1/1000 dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

• WB

Lab

Western blot - Anti-Annexin-4/ANXA4 antibody [EPR22929-208] (AB256456)

The expression molecular weight observed is consistent with what has been described in the literature (PMID : 16893879). Blocking/Diluting buffer and concentration : 5% NFDM/TBST. Exposure Time : Lanes 1-4 : 59 seconds; Lanes 5-8 : 125 seconds; Lane 9 : 10 seconds.

All lanes:

Western blot - Anti-Annexin-4/ANXA4 antibody [EPR22929-208] (ab256456) at 1/2000 dilution

Lane 1:

Human kidney lysate at 20 µg

Lane 2:

Human liver lysate at 20 µg

Lane 3:

Human lung lysate at 20 µg

Lane 4:

HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 5:

HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 6:

Mouse liver lysate at 20 µg

Lane 7:

Mouse kidney lysate at 20 µg

Lane 8:

Mouse lung lysate at 20 µg

Lane 9:

Rat kidney lysate at 20 µg

Secondary

Lanes 1 - 3:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Lanes 4 - 9:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 35 kDa

Observed band size: 31 kDa

false

• WB

Lab

Western blot - Anti-Annexin-4/ANXA4 antibody [EPR22929-208] (AB256456)

The expression molecular weight observed is consistent with what has been described in the literature (PMID : 16893879). Blocking/Diluting buffer and concentration : 5% NFDM/TBST. Exposure Time : 15 seconds.

All lanes:

Western blot - Anti-Annexin-4/ANXA4 antibody [EPR22929-208] (ab256456) at 1/2000 dilution

Lane 1:

C6 (rat glial tumor glial cell) whole cell lysate at 10 µg

Lane 2:

RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 10 µg

Lane 3:

PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate at 10 µg

Lane 4:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 35 kDa

Observed band size: 31 kDa

false