Anti-Annexin-2/ANXA2 抗体 [M298] (ab86709)
![Western blot - Anti-Annexin-2/ANXA2 antibody [M298] (ab86709)](https://www.abcam.co.jp/ps/products/86/ab86709/Images/ab86709-101759-anti-annexin-a2-antibody-m298-western-blot.jpg "Western blot - Anti-Annexin-2/ANXA2 antibody [M298] (ab86709)")
Key features and details
- Mouse monoclonal [M298] to Annexin-2/ANXA2
- Suitable for: WB, ICC/IF
- Reacts with: Rat
- Isotype: IgG1
製品の概要
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製品名
Anti-Annexin-2/ANXA2 antibody [M298]
Annexin-2/ANXA2 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [M298] to Annexin-2/ANXA2 -
由来種
Mouse -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Rat -
免疫原
Recombinant fragment corresponding to Human Annexin-2/ANXA2 (C terminal).
Database link: P07355 -
ポジティブ･コントロール
- WB: PC-12 cell lysate. ICC/IF: PC-12 cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 50% Glycerol, PBS -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
M298 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab86709の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 36 kDa.
|
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| ICC/IF |
1/50.
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| 特記事項 |
|---|
|
WB
1/1000. Detects a band of approximately 36 kDa. |
|
ICC/IF
1/50. |
ターゲット情報
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機能
Calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. May be involved in heat-stress response. -
配列類似性
Belongs to the annexin family.
Contains 4 annexin repeats. -
ドメイン
A pair of annexin repeats may form one binding site for calcium and phospholipid. -
翻訳後修飾
Phosphorylation of Tyr-24 enhances heat stress-induced translocation to the cell surface.
ISGylated. -
細胞内局在
Secreted > extracellular space > extracellular matrix > basement membrane. Melanosome. In the lamina beneath the plasma membrane. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Translocated from the cytoplasm to the cell surface through a Golgi-independent mechanism. - Information by UniProt
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参照データベース
- Entrez Gene: 56611 Rat
- SwissProt: Q07936 Rat
- Unigene: 90546 Rat
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別名
- Annexin A2 antibody
- Annexin II antibody
- Annexin II, heavy chain antibody
see all
画像
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Western blot - Anti-Annexin-2/ANXA2 antibody [M298] (ab86709)Lane 1 : Anti-Annexin-2/ANXA2 antibody [M298] (ab86709) at 1/250 dilution
Lane 2 : Anti-Annexin-2/ANXA2 antibody [M298] (ab86709) at 1/1000 dilution
All lanes : Rat PC12 cell lysate
Observed band size: 36 kDa why is the actual band size different from the predicted? -
![Immunocytochemistry/ Immunofluorescence - Anti-Annexin-2/ANXA2 antibody [M298] (ab86709)](https://www.abcam.co.jp/ps/products/86/ab86709/Images/ab86709-351579-anti-annexin-antibody-m298-immunocytochemistry-pc12-rat.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Annexin-2/ANXA2 antibody [M298] (ab86709)Paraformaldehyde-fixed, NP-40 permeabilized PC-12 (rat adrenal gland pheochromocytoma cell line) stained for Annexin-2/ANXA2 using ab86709 at 1/50 dilution in ICC/IF. Secondary antibody is a Goat antiMouse DyLight®594 conjugate.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab86709 は論文での使用が確認できていません。