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遺伝子ノックアウト細胞株 検証済リコンビナントRabMAb

Anti-Annexin-2/ANXA2 抗体 [EPR13053(2)(B)] - C-terminal (ab185957)

Submit a review Submit a question References (5)
Western blot - Anti-Annexin-2/ANXA2 antibody [EPR13053(2)(B)] - C-terminal (ab185957)
  • Western blot - Anti-Annexin-2/ANXA2 antibody [EPR13053(2)(B)] - C-terminal (ab185957)
  • Western blot - Anti-Annexin-2/ANXA2 antibody [EPR13053(2)(B)] - C-terminal (ab185957)
  • Western blot - Anti-Annexin-2/ANXA2 antibody [EPR13053(2)(B)] - C-terminal (ab185957)
  • Anti-Annexin-2/ANXA2 antibody [EPR13053(2)(B)] - C-terminal (ab185957)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13053(2)(B)] to Annexin-2/ANXA2 - C-terminal
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-Annexin-2/ANXA2 antibody [EPR13053(2)(B)] - C-terminal
    Annexin-2/ANXA2 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR13053(2)(B)] to Annexin-2/ANXA2 - C-terminal

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WBmore details

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: K562, 293, HeLa, HepG2, SH-SY5Y, C6, RAW264.7, PC12 and NIH 3T3 cell lysates.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab185957の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000 - 1/10000. Detects a band of approximately 36 kDa (predicted molecular weight: 39 kDa).
特記事項
WB
1/1000 - 1/10000. Detects a band of approximately 36 kDa (predicted molecular weight: 39 kDa).

ターゲット情報

  • 機能

    Calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. May be involved in heat-stress response.

  • 配列類似性

    Belongs to the annexin family.
    Contains 4 annexin repeats.

  • ドメイン

    A pair of annexin repeats may form one binding site for calcium and phospholipid.

  • 翻訳後修飾

    Phosphorylation of Tyr-24 enhances heat stress-induced translocation to the cell surface.
    ISGylated.

  • 細胞内局在

    Secreted > extracellular space > extracellular matrix > basement membrane. Melanosome. In the lamina beneath the plasma membrane. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Translocated from the cytoplasm to the cell surface through a Golgi-independent mechanism.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • Annexin A2 antibody
    • Annexin II antibody
    • Annexin II, heavy chain antibody
    • Annexin-2 antibody
    • ANX 2 antibody
    • ANX2 antibody
    • ANX2L4 antibody
    • ANXA2 antibody
    • ANXA2_HUMAN antibody
    • arylsulfatase B antibody
    • CAL1H antibody
    • Calpactin I heavy chain antibody
    • calpactin I heavy polypeptide (p36) antibody
    • Calpactin I heavy polypeptide antibody
    • Calpactin-1 heavy chain antibody
    • chromobindin 8 antibody
    • Chromobindin-8 antibody
    • Epididymis secretory protein Li 270 antibody
    • HEL S 270 antibody
    • LIP2 antibody
    • Lipocortin II antibody
    • LPC2 antibody
    • LPC2D antibody
    • p36 antibody
    • P36 protein antibody
    • PAP-IV antibody
    • Placental anticoagulant protein IV antibody
    • Protein I antibody
    see all

画像

  • Western blot - Anti-Annexin-2/ANXA2 antibody [EPR13053(2)(B)] - C-terminal (ab185957)
    Western blot - Anti-Annexin-2/ANXA2 antibody [EPR13053(2)(B)] - C-terminal (ab185957)
    All lanes : Anti-Annexin-2/ANXA2 antibody [EPR13053(2)(B)] - C-terminal (ab185957) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : ANXA2 knockout HeLa cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : SH-SY5Y cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 39 kDa
    Observed band size: 37 kDa why is the actual band size different from the predicted?



    Lanes 1 - 4: Merged signal (red and green). Green - ab185957 observed at 37 kDa. Red - loading control, ab7291 (Mouse anti-Alpha Tubulin [DM1A] observed at 55kDa.

    ab185957 was shown to react with Annexin-2/ANXA2 in wild-type HeLa cells in western blot. Loss of signal was observed when ANXA2 knockout sample was used. Wild-type and ANXA2 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab185957 and ab7291 (Mouse anti-Alpha Tubulin [DM1A] overnight at 4°C at a 1 in 1000 Dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-Annexin-2/ANXA2 antibody [EPR13053(2)(B)] - C-terminal (ab185957)
    Western blot - Anti-Annexin-2/ANXA2 antibody [EPR13053(2)(B)] - C-terminal (ab185957)
    All lanes : Anti-Annexin-2/ANXA2 antibody [EPR13053(2)(B)] - C-terminal (ab185957) at 1/1000 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : 293 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 39 kDa

  • Western blot - Anti-Annexin-2/ANXA2 antibody [EPR13053(2)(B)] - C-terminal (ab185957)
    Western blot - Anti-Annexin-2/ANXA2 antibody [EPR13053(2)(B)] - C-terminal (ab185957)
    Anti-Annexin-2/ANXA2 antibody [EPR13053(2)(B)] - C-terminal (ab185957) at 1/10000 dilution + HeLa cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 39 kDa

  • Western blot - Anti-Annexin-2/ANXA2 antibody [EPR13053(2)(B)] - C-terminal (ab185957)
    Western blot - Anti-Annexin-2/ANXA2 antibody [EPR13053(2)(B)] - C-terminal (ab185957)
    All lanes : Anti-Annexin-2/ANXA2 antibody [EPR13053(2)(B)] - C-terminal (ab185957) at 1/1000 dilution

    Lane 1 : C6 cell lysate
    Lane 2 : RAW264.7 cell lysate
    Lane 3 : PC12 cell lysate
    Lane 4 : NIH 3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 39 kDa

  • Anti-Annexin-2/ANXA2 antibody [EPR13053(2)(B)] - C-terminal (ab185957)
    Anti-Annexin-2/ANXA2 antibody [EPR13053(2)(B)] - C-terminal (ab185957)

参考文献 (5)

ab185957 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab185957 は 5 報の論文で使用されています。

  • Zhao A  et al. Prognostic values of Annexins and validation of the influence on cell proliferation, migration, and invasion in uveal melanoma. Am J Transl Res 15:3131-3151 (2023). PubMed: 37303667
  • Cao H  et al. Super-enhancer-associated INSM2 regulates lipid metabolism by modulating mTOR signaling pathway in neuroblastoma. Cell Biosci 12:158 (2022). PubMed: 36114560
  • Zhang Q  et al. Clusterin as a serum biomarker candidate contributes to the lung fibroblasts activation in chronic obstructive pulmonary disease. Chin Med J (Engl) 135:1076-1086 (2022). PubMed: 35191419
  • Sun XY  et al. Regulatory Effects of Damaged Renal Epithelial Cells After Repair by Porphyra yezoensis Polysaccharides with Different Sulfation Degree on the Calcium Oxalate Crystal-Cell Interaction. Int J Nanomedicine 16:8087-8102 (2021). PubMed: 34934314
  • Salibe-Filho W  et al. Shear stress-exposed pulmonary artery endothelial cells fail to upregulate HSP70 in chronic thromboembolic pulmonary hypertension. PLoS One 15:e0242960 (2020). PubMed: 33270690

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