Anti-Annexin-2/ANXA2 抗体 (ab154113)
Key features and details
- Rabbit polyclonal to Annexin-2/ANXA2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Annexin-2/ANXA2 antibody
Annexin-2/ANXA2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Annexin-2/ANXA2 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Recombinant fragment corresponding to Human Annexin-2/ANXA2 aa 19-237.
Database link: P07355 -
ポジティブ･コントロール
- A549, HCT116, GL261, NIH 3T3, C2C12 and Rat2 whole cell lysates; A431 cells; Human colon cancer tissue
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab154113の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 38 kDa.
|
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| IHC-P |
1/100 - 1/1000.
|
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| ICC/IF |
1/100 - 1/1000.
|
| 特記事項 |
|---|
|
WB
1/500 - 1/3000. Predicted molecular weight: 38 kDa. |
|
IHC-P
1/100 - 1/1000. |
|
ICC/IF
1/100 - 1/1000. |
ターゲット情報
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機能
Calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. May be involved in heat-stress response. -
配列類似性
Belongs to the annexin family.
Contains 4 annexin repeats. -
ドメイン
A pair of annexin repeats may form one binding site for calcium and phospholipid. -
翻訳後修飾
Phosphorylation of Tyr-24 enhances heat stress-induced translocation to the cell surface.
ISGylated. -
細胞内局在
Secreted > extracellular space > extracellular matrix > basement membrane. Melanosome. In the lamina beneath the plasma membrane. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Translocated from the cytoplasm to the cell surface through a Golgi-independent mechanism. - Information by UniProt
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参照データベース
- Entrez Gene: 302 Human
- Entrez Gene: 12306 Mouse
- Entrez Gene: 56611 Rat
- Omim: 151740 Human
- SwissProt: P07355 Human
- SwissProt: P07356 Mouse
- SwissProt: Q07936 Rat
- Unigene: 511605 Human
see all -
別名
- Annexin A2 antibody
- Annexin II antibody
- Annexin II, heavy chain antibody
see all
画像
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Western blot - Anti-Annexin-2/ANXA2 antibody (ab154113)All lanes : Anti-Annexin-2/ANXA2 antibody (ab154113) at 1/1000 dilution
Lane 1 : WT HeLa cell extract
Lane 2 : Annexin-2/ANXA2 Knockout HeLa cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 38 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-Annexin-2/ANXA2 antibody (ab154113)Immunofluorescent analysis of paraformaldehyde fixed A431 cells labeling Annexin-2/ANXA2 with ab154113 at 1/200 dilution.
The image in the lower panel was merged with DNA probe. -
Western blot - Anti-Annexin-2/ANXA2 antibody (ab154113)Anti-Annexin-2/ANXA2 antibody (ab154113) at 1/1000 dilution + Rat2 whole cell lysate at 30 µg
Predicted band size: 38 kDa
10% SDS PAGE -
Western blot - Anti-Annexin-2/ANXA2 antibody (ab154113)All lanes : Anti-Annexin-2/ANXA2 antibody (ab154113) at 1/1000 dilution
Lane 1 : Non-transfected (-) 293T whole cell lysates
Lane 2 : 293T transfected with human Annexin-1/ANXA1 whole cell lysates
Lane 3 : 293T transfected with human Annexin-2/ANXA2 whole cell lysates
Lysates/proteins at 30 µg per lane.
Predicted band size: 38 kDa -
Western blot - Anti-Annexin-2/ANXA2 antibody (ab154113)All lanes : Anti-Annexin-2/ANXA2 antibody (ab154113) at 1/1000 dilution
Lane 1 : GL261 whole cell lysate
Lane 2 : NIH 3T3 whole cell lysate
Lane 3 : C2C12 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 38 kDa
10% SDS PAGE -
Western blot - Anti-Annexin-2/ANXA2 antibody (ab154113)All lanes : Anti-Annexin-2/ANXA2 antibody (ab154113) at 1/1000 dilution
Lane 1 : A549 whole cell lysate
Lane 2 : HCT116 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 38 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin-2/ANXA2 antibody (ab154113)Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue labeling Annexin-2/ANXA2 with ab154113 at 1/500 dilution.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab154113 は 1 報の論文で使用されています。
- Demonbreun AR et al. Recombinant annexin A6 promotes membrane repair and protects against muscle injury. J Clin Invest 129:4657-4670 (2019). PubMed: 31545299