Anti-Annexin-11/ANXA11 抗体 (ab166846)
Key features and details
- Mouse polyclonal to Annexin-11/ANXA11
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Annexin-11/ANXA11 antibody
Annexin-11/ANXA11 一次抗体 製品一覧 -
製品の詳細
Mouse polyclonal to Annexin-11/ANXA11 -
由来種
Mouse -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Recombinant full length protein within Human Annexin-11/ANXA11 aa 1-550. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P50995 -
ポジティブ･コントロール
- Human pancreas tissue lysate; Annexin-11/ANXA11 transfected 293T cell lysate; HeLa cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
pH: 7.4
Constituent: 99% PBS -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab166846の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 54 kDa.
|
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| ICC/IF |
Use a concentration of 10 µg/ml.
|
| 特記事項 |
|---|
|
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 54 kDa. |
|
ICC/IF
Use a concentration of 10 µg/ml. |
ターゲット情報
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機能
Binds specifically to calcyclin in a calcium-dependent manner (By similarity). Required for midbody formation and completion of the terminal phase of cytokinesis. -
配列類似性
Belongs to the annexin family.
Contains 4 annexin repeats. -
ドメイン
A pair of annexin repeats may form one binding site for calcium and phospholipid. -
細胞内局在
Cytoplasm. Melanosome. Nucleus envelope. Nucleus > nucleoplasm. Cytoplasm > cytoskeleton > spindle. Found throughout the nucleoplasm at interphase and during mitosis concentrates around the mitotic apparatus (By similarity). Elevation of intracellular calcium causes relocalization from the nucleoplasm to the nuclear envelope, with little effect on the cytoplasmic pool. Localization to the nuclear envelope is cell-cycle dependent. - Information by UniProt
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参照データベース
- Entrez Gene: 311 Human
- Omim: 602572 Human
- SwissProt: P50995 Human
- Unigene: 530291 Human
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別名
- 56 kDa autoantigen antibody
- Annexin A11 antibody
- Annexin XI antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-Annexin-11/ANXA11 antibody (ab166846)
Immunofluorescent analysis of HeLa cells, labeling Annexin-11/ANXA11 with ab166846 at 10µg/ml.
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Western blot - Anti-Annexin-11/ANXA11 antibody (ab166846)Anti-Annexin-11/ANXA11 antibody (ab166846) at 1 µg/ml + Human Pancreas tissue lysate at 50 µg
Secondary
Goat anti-Mouse IgG HRP at 1/2500 dilution
Predicted band size: 54 kDa -
Western blot - Anti-Annexin-11/ANXA11 antibody (ab166846)All lanes : Anti-Annexin-11/ANXA11 antibody (ab166846) at 1 µg/ml
Lane 1 : Annexin-11/ANXA11 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 15 µl per lane.
Secondary
All lanes : Goat anti-Mouse IgG HRP at 1/2500 dilution
Predicted band size: 54 kDa
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (0)
ab166846 は論文での使用が確認できていません。
