AB211872

Anti-Anillin 抗体 [CL0303]

Name: Anti-Anillin 抗体 [CL0303] (ab211872) | Abcam
Brand: Abcam Limited
SKU: AB211872
Price: 72000 JPY
Availability: InStock
Rating: 4 (4 reviews)

Anti-Anillin antibody [CL0303]

(4 Reviews)

(17 Publications)

Mouse Monoclonal Anillin antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 17 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ANLN aa 300-500.

別名を表示する

Anillin, ANLN

14 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Anillin antibody [CL0303] (AB211872)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Anillin antibody [CL0303] (AB211872)

Immunohistochemical analysis of paraffin-embedded, formalin fixed Human testis tissue labeling Anillin with ab211872 at 1/50 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-Anillin antibody [CL0303] (AB211872)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Anillin antibody [CL0303] (AB211872)

Immunofluorescent analysis of formalin fixed, Triton X-100 permealized U251 cells labeling Anillin with ab211872 at 4 μg/ml dilution showing cell cycle dependent nuclear (without nucleoli) staining in green. Microtubules are visualized in red.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Anillin antibody [CL0303] (AB211872)

Immunohistochemical analysis of paraffin-embedded, formalin fixed Human tonsil tissue labeling Anillin with ab211872 at 1/50 dilution.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Anillin antibody [CL0303] (AB211872)

Immunofluorescent analysis of formalin fixed, Triton X-100 permealized MCF7 cells labeling Anillin with ab211872 at 4 μg/ml dilution showing cell cycle dependent nuclear (without nucleoli) staining in green. Microtubules are visualized in red.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Anillin antibody [CL0303] (AB211872)

Immunofluorescent analysis of formalin fixed, Triton X-100 permealized A431 cells labeling Anillin with ab211872 at 4 μg/ml dilution showing cell cycle dependent nuclear (without nucleoli) staining in green. Microtubules are visualized in red.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Anillin antibody [CL0303] (AB211872)

Immunohistochemical analysis of paraffin-embedded, formalin fixed Human colorectal cancer tissue labeling Anillin with ab211872 at 1/50 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Anillin antibody [CL0303] (AB211872)

Immunohistochemical analysis of paraffin-embedded, formalin fixed Human colon tissue labeling Anillin with ab211872 at 1/50 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Anillin antibody [CL0303] (AB211872)

Negative control : Immunohistochemical analysis of paraffin-embedded, formalin fixed Human liver tissue labeling Anillin with ab211872 at 1/50 dilution shows absence of staining.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Anillin antibody [CL0303] (AB211872)

Immunofluorescent analysis of formalin fixed, Triton X-100 permealized U2OS cells labeling Anillin with ab211872 at 4 μg/ml dilution showing cell cycle dependent nuclear (without nucleoli) staining in green. Microtubules are visualized in red.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Anillin antibody [CL0303] (AB211872)

Immunofluorescent analysis of formalin fixed, Triton X-100 permealized HeLa cells labeling Anillin with ab211872 at 4 μg/ml dilution showing cell cycle dependent nuclear (without nucleoli) staining in green. Microtubules are visualized in red.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Anillin antibody [CL0303] (AB211872)

Immunohistochemical analysis of paraffin-embedded, formalin fixed Human stomach tissue labeling Anillin with ab211872 at 1/50 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Anillin antibody [CL0303] (AB211872)

Immunohistochemical analysis of paraffin-embedded, formalin fixed Human lung cancer tissue labeling Anillin with ab211872 at 1/50 dilution.

Supplier Data

Western blot - Anti-Anillin antibody [CL0303] (AB211872)

Downregulation of antibody signal confirms target specificity. Remaining % intensity, relative control lane, is indicated. Anti-GAPDH monoclonal antibody was used as loading control.

All lanes:

Western blot - Anti-Anillin antibody [CL0303] (ab211872)

Lane 1:

U-251 cells, transfected with control siRNA

Lane 2:

U-251 cells, transfected with target specific siRNA probe #1

Lane 3:

U-251 cells, transfected with target specific siRNA probe #2

Predicted band size: 124 kDa

false

Supplier Data

Western blot - Anti-Anillin antibody [CL0303] (AB211872)

All lanes:

Western blot - Anti-Anillin antibody [CL0303] (ab211872) at 1/500 dilution

All lanes:

U251 cell line lysate

Predicted band size: 124 kDa

false

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

CL0303

アイソタイプ

IgG1

キャリアフリー

交差種

Human

アプリケーション

IHC-P, ICC/IF, WB

applications

免疫原

Recombinant Fragment Protein within Human ANLN aa 300-500. The exact immunogen used to generate this antibody is proprietary information.

Q9NQW6

エピトープ

Binds to an epitope located within the peptide sequence LRGRFDKGNIWSAEK (438-452 aa) as determined by overlapping synthetic peptides.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "2-10 µg/mL", "ICCIF-species-notes": "" } } }

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製品の詳細

Marker for Nucleus without Nucleoli

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Anillin also known as ANLN is a conserved protein with a molecular weight of approximately 124 kDa. It plays an important role in the regulation of the cytoskeleton particularly during cell division. Anillin localizes mainly at the cleavage furrow during cytokinesis and interacts with several cytoskeletal components including actin filaments and myosin. Expression of Anillin occurs in various tissues but it is most abundant in rapidly dividing cells such as those found in cancers and during embryonic development.

Biological function summary

Anillin acts as a scaffold protein coordinating the assembly of the contractile ring during mitosis and cytokinesis. By binding to actin and myosin and sometimes septins Anillin ensures the successful completion of cell division. It forms part of a complex with these proteins promoting stability and functionality of the cleavage furrow. Anillin's structural role is critical for the maintenance and integrity of the dividing cell's structure.

Pathways

Anillin plays a role in the RHO signaling pathway and the cytoskeleton remodeling pathway. It interacts with other proteins in these pathways such as RhoA and Ect2 facilitating the precise control of contractile ring formation. These interactions recruit additional factors to the cleavage site organizing cellular machinery for the final stages of cell division. Anillin's involvement in these pathways underlines its significance in both cell signaling and structural organization.

Anillin has been correlated with cancer progression and metastasis. Overexpression of Anillin has been observed in several types of cancers including breast and lung cancer. It interacts with proteins such as Aurora B kinase linking Anillin to tumor cell proliferation and survival. Understanding Anillin's function and interactions can offer insights into potential therapeutic strategies for targeting dysregulated cell division in cancer.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Required for cytokinesis (PubMed : 16040610). Essential for the structural integrity of the cleavage furrow and for completion of cleavage furrow ingression. Plays a role in bleb assembly during metaphase and anaphase of mitosis (PubMed : 23870127). May play a significant role in podocyte cell migration (PubMed : 24676636).

See full target information ANLN

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文献 (17)

Recent publications for all applications. Explore the full list and refine your search

Aging and disease : PubMed40423641

2025

Anillin Recedes in p53-Dependent Senescence of Tumor Cells and Reappears in Cells Escaping from Senescence.

Applications

Unspecified application

Species

Unspecified reactive species

Tomasz Buko,Karolina Staniak,Magdalena Dudkowska,Dorota Janiszewska,Dominika Dębowska,Agnieszka Gadecka,Anna Bielak-Zmijewska

iScience 28:111633 PubMed39967877

2025

ANLN promotes head and neck squamous cell carcinoma progression by upregulating PD-L1 via the ERK-MAPK pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Wang,Junrong Wang,Nana Wang,Xueying Wang,Ming Song,Yang Zhou,Ziyuan Wang,Hongxue Meng,Erliang Guo,Susheng Miao

Nature communications 16:1271 PubMed39894879

2025

Nuclear ANLN regulates transcription initiation related Pol II clustering and target gene expression.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Fei Cao,Hui Wang,Yong Sun,Bei-Bei Tong,Wen-Qi Shi,Liu Peng,Yi-Meng Zhang,Yu-Qiu Wu,Teng Fu,Hua-Yan Zou,Kai Zhang,Li-Yan Xu,En-Min Li

Asian journal of urology 11:569-574 PubMed39533998

2024

Anillin actin-binding protein expression correlates with poor prognosis for prostate cancer patients.

Applications

Unspecified application

Species

Unspecified reactive species

Shinichiro Yamamoto,Daisuke Obinata,Kenichi Takayama,Daigo Funakoshi,Kyoko Fujiwara,Makoto Hara,Satoru Takahashi,Satoshi Inoue

Acta biochimica et biophysica Sinica 56:210-222 PubMed38273783

2024

lncRNA CYTOR promotes lung adenocarcinoma gemcitabine resistance and epithelial-mesenchymal transition by sponging miR-125a-5p and upregulating ANLN and RRM2.

Applications

Unspecified application

Species

Unspecified reactive species

Qijun Cao,Haixia Wang,Jialong Zhu,Chen Qi,Hairong Huang,Xiaoyuan Chu

Molecular medicine reports 28: PubMed37539739

2023

Knockdown of ANLN inhibits the progression of lung adenocarcinoma via pyroptosis activation.

Applications

Unspecified application

Species

Unspecified reactive species

Li Sheng,Yanhai Kang,Denglin Chen,Linyang Shi

Translational andrology and urology 12:176-186 PubMed36915888

2023

Identification and validation of immunohistochemical marker panels to predict the prognosis of muscle invasive bladder cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yidie Ying,Ziwei Wang,Yuxin Tan,Haotian Cao,Hongliang Gao,Zhensheng Zhang,Shuxiong Zeng,Chuanliang Xu

Molecular & cellular proteomics : MCP 22:100485 PubMed36549590

2022

Native Size-Exclusion Chromatography-Based Mass Spectrometry Reveals New Components of the Early Heat Shock Protein 90 Inhibition Response Among Limited Global Changes.

Applications

Unspecified application

Species

Unspecified reactive species

Rahul S Samant,Silvia Batista,Mark Larance,Bugra Ozer,Christopher I Milton,Isabell Bludau,Estelle Wu,Laura Biggins,Simon Andrews,Alexia Hervieu,Harvey E Johnston,Bissan Al-Lazikhani,Angus I Lamond,Paul A Clarke,Paul Workman

Cancer cell international 22:390 PubMed36482354

2022

Exosomal LINC00460/miR-503-5p/ANLN positive feedback loop aggravates pancreatic cancer progression through regulating T cell-mediated cytotoxicity and PD-1 checkpoint.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Yao,Ruoyu Gao,Minghan Luo,Defeng Li,Liliangzi Guo,Zichao Yu,Feng Xiong,Cheng Wei,Benhua Wu,Zhenglei Xu,Dingguo Zhang,Jianyao Wang,Lisheng Wang

Molecular therapy : the journal of the American Society of Gene Therapy 30:3477-3498 PubMed35791879

2022

CircRNA Samd4 induces cardiac repair after myocardial infarction by blocking mitochondria-derived ROS output.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Zheng,Senlin Huang,Guoquan Wei,Yili Sun,Chuling Li,Xiaoyun Si,Yijin Chen,Zhenquan Tang,Xinzhong Li,Yanmei Chen,Wangjun Liao,Yulin Liao,Jianping Bin

View all publications

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Abcam product promise

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保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Anillin 抗体 [CL0303]

Anti-Anillin antibody [CL0303]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Anillin antibody [CL0303] (AB211872)

Immunocytochemistry/ Immunofluorescence - Anti-Anillin antibody [CL0303] (AB211872)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Anillin antibody [CL0303] (AB211872)

Immunocytochemistry/ Immunofluorescence - Anti-Anillin antibody [CL0303] (AB211872)

Immunocytochemistry/ Immunofluorescence - Anti-Anillin antibody [CL0303] (AB211872)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Anillin antibody [CL0303] (AB211872)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Anillin antibody [CL0303] (AB211872)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Anillin antibody [CL0303] (AB211872)

Immunocytochemistry/ Immunofluorescence - Anti-Anillin antibody [CL0303] (AB211872)

Immunocytochemistry/ Immunofluorescence - Anti-Anillin antibody [CL0303] (AB211872)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Anillin antibody [CL0303] (AB211872)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Anillin antibody [CL0303] (AB211872)

Western blot - Anti-Anillin antibody [CL0303] (AB211872)

Western blot - Anti-Anillin antibody [CL0303] (AB211872)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

エピトープ

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (17)

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Abcam product promise