Anti-Anillin 抗体 (ab5910)
Key features and details
- Goat polyclonal to Anillin
- Suitable for: Flow Cyt (Intra), ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Anillin antibody
Anillin 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to Anillin -
由来種
Goat -
アプリケーション
適用あり: Flow Cyt (Intra), ICC/IF, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Cow -
免疫原
Synthetic peptide corresponding to Human Anillin aa 1100 to the C-terminus (C terminal). (NP_001271230.1; NP_001271231.1)
Database link: Q9NQW6 -
ポジティブ・コントロール
- ICC/IF: U2OS cells. Flow Cyt (intra): MCF7 cells. IHC-P: Human kidney tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab5910の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt (Intra) |
Use a concentration of 10 µg/ml.
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ICC/IF | (3) |
Use a concentration of 10 µg/ml.
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IHC-P | (1) |
Use a concentration of 3 - 10 µg/ml.
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特記事項 |
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Flow Cyt (Intra)
Use a concentration of 10 µg/ml. |
ICC/IF
Use a concentration of 10 µg/ml. |
IHC-P
Use a concentration of 3 - 10 µg/ml. |
ターゲット情報
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機能
Required for cytokinesis. Essential for the structural integrity of the cleavage furrow and for completion of cleavage furrow ingression. -
組織特異性
Ubiquitously expressed. Present at highest levels in the brain, at high levels in the placenta and testis, at intermediate levels in the intestine, ovary, skeletal muscle and thymus and at lower levels in heart, kidney, liver, lung, pancreas, prostate and spleen. Overexpressed in many tumor types including breast, colorectal, endometrial, hepatic, kidney, lung, ovarian and pancreatic tumors. -
配列類似性
Contains 1 PH domain. -
発生段階
Expressed in fetal brain, heart, kidney, liver, lung, skeletal muscle, spleen and thymus. In dividing cells expression increases during S and G2 phases, peaks at mitosis and subsequently drops as cells enter G1 phase. -
翻訳後修飾
Phosphorylated during mitosis.
Ubiquitinated, and this requires FZR1/CDH1. -
細胞内局在
Nucleus. Cytoplasm > cytoskeleton. Cytoplasm > cell cortex. Mainly found in the nucleus during interphase. Colocalizes with cortical F-actin upon nuclear envelope breakdown in mitosis and subsequently concentrates in the area of the prospective contractile ring in anaphase. This pattern persists until telophase, when the protein becomes concentrated in the midbody. - Information by UniProt
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参照データベース
- Entrez Gene: 54443 Human
- Entrez Gene: 68743 Mouse
- Omim: 616027 Human
- SwissProt: Q9NQW6 Human
- SwissProt: Q8K298 Mouse
- Unigene: 62180 Human
- Unigene: 282751 Mouse
- Unigene: 453238 Mouse
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別名
- Actin binding protein scraps homolog Drosophila antibody
- Actin binding protein anillin antibody
- Actin-binding protein anillin antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Anillin antibody (ab5910)
ab5910 at 10ug/ml staining Anillin in human kidney tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent antigen retrieval in microwave and in Tris/EDTA buffer (pH 9.0). HRP-staining procedure was used for detection.
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Flow cytometric analysis of paraformaldehyde fixed MCF7 cells (blue line) and permeabilized with 0.5% Triton using ab5910 . Primary incubation 1hr (10µg/ml) followed by Alexa Fluor 488 secondary antibody (1µg/ml). IgG control: Unimmunized goat IgG (black line) fol
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Immunocytochemistry/Immunofluorescence analysis of U2OS cells labelling Anillin with ab5910 at 10 µg/ml. Cells were paraformaldehyde fixed and permeabilized with 0.15% triton. Primary antibody icubation was for 1 hour followed by incubation with an Alexa Fluor® 488-conjugated secondary antibody at 2 µg/ml. DAPI nuclear counterstain was used.
Negative control: Unimmunized goat IgG (10 µg/ml) followed by an Alexa Fluor® 488-conjugated secondary antibody (2 µg/ml).
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Immunocytochemistry/ Immunofluorescence - Anti-Anillin antibody (ab5910)This image is courtesy of an Abreview submitted by Manoj B Menon
ab14404 staining Anillinin Mouse fibroblast cellsby ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with methanol and blocked with 4% BSA for 2 hours at 4°C. Samples were incubated with primary antibody (1/25 in PBS + 1% BSA) for 2 hour at 25°C. An Alexa Fluor®488-conjugated Donkey anti-goat polyclonal(1/500) was used as the secondary antibody.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (8)
ab5910 は 8 報の論文で使用されています。
- Han L et al. Lamin B2 Levels Regulate Polyploidization of Cardiomyocyte Nuclei and Myocardial Regeneration. Dev Cell 53:42-59.e11 (2020). PubMed: 32109383
- Kalebic N et al. Neocortical Expansion Due to Increased Proliferation of Basal Progenitors Is Linked to Changes in Their Morphology. Cell Stem Cell 24:535-550.e9 (2019). PubMed: 30905618
- Luján P et al. PRL-3 disrupts epithelial architecture by altering the post-mitotic midbody position. J Cell Sci 129:4130-4142 (2016). PubMed: 27656108
- Zhou L et al. Anxiety and depression with neurogenesis defects in exchange protein directly activated by cAMP 2-deficient mice are ameliorated by a selective serotonin reuptake inhibitor, Prozac. Transl Psychiatry 6:e881 (2016). WB . PubMed: 27598965
- Stoeckelhuber M et al. Human axillary apocrine glands: proteins involved in the apocrine secretory mechanism. Histol Histopathol 26:177-84 (2011). IHC-P ; Human . PubMed: 21154231
- Stubbs D et al. Neurovascular congruence during cerebral cortical development. Cereb Cortex 19 Suppl 1:i32-41 (2009). PubMed: 19386634
- Bantubungi K et al. Stem cell factor and mesenchymal and neural stem cell transplantation in a rat model of Huntington's disease. Mol Cell Neurosci 37:454-70 (2008). PubMed: 18083596
- Hall PA et al. The septin-binding protein anillin is overexpressed in diverse human tumors. Clin Cancer Res 11:6780-6 (2005). IHC-P ; Human . PubMed: 16203764