AB229005

Anti-Angiotensinogen 抗体 [EPR22199-203]

Name: Anti-Angiotensinogen 抗体 [EPR22199-203] (ab229005) | Abcam
Brand: Abcam Limited
SKU: AB229005
Price: 45000 JPY
Availability: InStock
Rating: 5 (2 reviews)

Anti-Angiotensinogen antibody [EPR22199-203]

RabMAb
Recombinant
詳細を見る

(2 Reviews)

(1 Publication)

Rabbit Recombinant Monoclonal Angiotensinogen antibody. Suitable for IP, WB, IHC-P and reacts with Mouse samples. Cited in 1 publication.

別名を表示する

Serpina8, Agt, Angiotensinogen, Serpin A8

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Angiotensinogen antibody [EPR22199-203] (AB229005)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Angiotensinogen antibody [EPR22199-203] (AB229005)

Immunohistochemical analysis of paraffin-embedded mouse testis tissue stained for Angiotensinogen with ab229005 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP) secondary antibody. Cytoplasmic staining in the Leydig cells of mouse testis (PMID : 20361351) is observed. Counterstained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG ready to use.

Heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Angiotensinogen antibody [EPR22199-203] (AB229005)

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue stained for Angiotensinogen with ab229005 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP) secondary antibody. Cytoplasmic staining in the proximal tubules of mouse kidney (PMID : 7648615) is observed. Counterstained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG ready to use.

Heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Supplier Data

Immunoprecipitation - Anti-Angiotensinogen antibody [EPR22199-203] (AB229005)

Angiotensinogen was immunoprecipitated from 0.35 mg mouse liver tissue lysates with ab229005 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab229005 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/5000 dilution.

Lane 1 : Mouse liver lysate (10 μg) (Input).
Lane 2 : ab29005 IP in mouse liver lysate (+).
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab229005 in mouse liver lysate (-).

Blocking/D ilution buffer : 5% NFDM/TBST.
Exposure time : 10 seconds.

All lanes:

Immunoprecipitation - Anti-Angiotensinogen antibody [EPR22199-203] (ab229005)

Predicted band size: 34 kDa,45 kDa,47 kDa,53 kDa,55 kDa,57 kDa

Observed band size: 40 kDa,48 kDa

false

Supplier Data

Western blot - Anti-Angiotensinogen antibody [EPR22199-203] (AB229005)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Angiotensinogen antibody [EPR22199-203] (ab229005) at 1/1000 dilution

Lane 1:

Mouse liver tissue lysate at 20 µg

Lane 2:

Mouse kidney tissue lysate at 20 µg

Lane 3:

Mouse heart tissue lysate at 20 µg

Lane 4:

Mouse brain tissue lysate at 20 µg

Lane 5:

Mouse lung tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 53 kDa

false

Exposure time: 114s

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR22199-203

アイソタイプ

IgG

キャリアフリー

交差種

Mouse

アプリケーション

IP, IHC-P, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2000", "IHCP-species-notes": "" } } }

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製品の詳細

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Angiotensinogen also known as AGT is a precursor to angiotensin peptides and plays a mechanical role in the renin-angiotensin system. It is a glycoprotein with a mass of approximately 60 kDa and is mainly produced in the liver. This protein circulates in the bloodstream where it waits for renin to cleave it. Angiotensinogen's presence is significant in various tissues but primarily it functions within the plasma after hepatic secretion.

Biological function summary

Angiotensinogen is critical in the regulation of blood pressure and fluid balance. It serves as the substrate for renin an enzyme that initiates a cascade leading to the production of angiotensin I which further converts into active forms like angiotensin II. Angiotensinogen does not operate as part of a larger protein complex but interacts closely with enzymes and other substrates to mediate its effects on the cardiovascular system.

Pathways

Angiotensinogen is an essential component of the renin-angiotensin-aldosterone system (RAAS) which helps regulate blood pressure and electrolyte balance. The pathway involves the conversion of angiotensinogen to angiotensin I by renin followed by the conversion to angiotensin II by angiotensin-converting enzyme (ACE). This system also has connections with proteins such as aldosterone which is involved in sodium and water retention influencing cardiovascular health.

Angiotensinogen links to hypertension and cardiovascular diseases. The protein's interaction with renin and ACE results in the formation of angiotensin II a potent vasoconstrictor that increases blood pressure. Elevated levels of angiotensinogen are often observed in individuals with high 117/85 blood pressure indicating a risk for chronic conditions. Managing angiotensinogen levels through RAAS modulation is a therapeutic strategy for controlling these disorders.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Essential component of the renin-angiotensin system (RAS), a potent regulator of blood pressure, body fluid and electrolyte homeostasis.. Angiotensin-2. Acts directly on vascular smooth muscle as a potent vasoconstrictor, affects cardiac contractility and heart rate through its action on the sympathetic nervous system, and alters renal sodium and water absorption through its ability to stimulate the zona glomerulosa cells of the adrenal cortex to synthesize and secrete aldosterone. Acts by binding to angiotensin receptors AGTR1 and AGTR2. Also binds the DEAR/FBXW7-AS1 receptor.. Angiotensin-3. Stimulates aldosterone release.. Angiotensin 1-7. Is a ligand for the G-protein coupled receptor MAS1 (PubMed : 12829792). Has vasodilator and antidiuretic effects. Has an antithrombotic effect that involves MAS1-mediated release of nitric oxide from platelets (PubMed : 12829792, PubMed : 18026570).

See full target information Agt

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 23: PubMed35055180

2022

Biallelic Variants in Cause a Severe Infantile Metabolic Disorder Affecting Mitochondrial Function.

Applications

Unspecified application

Species

Unspecified reactive species

Nicole J Van Bergen,Daniella H Hock,Lucy Spencer,Sean Massey,Tegan Stait,Zornitza Stark,Sebastian Lunke,Ain Roesley,Heidi Peters,Joy Yaplito Lee,Anna Le Fevre,Oliver Heath,Cristina Mignone,Joseph Yuan-Mou Yang,Monique M Ryan,Colleen D'Arcy,Margot Nash,Sile Smith,Nikeisha J Caruana,David R Thorburn,David A Stroud,Susan M White,John Christodoulou,Natasha J Brown

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Angiotensinogen 抗体 [EPR22199-203]

Anti-Angiotensinogen antibody [EPR22199-203]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Angiotensinogen antibody [EPR22199-203] (AB229005)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Angiotensinogen antibody [EPR22199-203] (AB229005)

Immunoprecipitation - Anti-Angiotensinogen antibody [EPR22199-203] (AB229005)

Western blot - Anti-Angiotensinogen antibody [EPR22199-203] (AB229005)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (1)

代替製品

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Primary Antibodies

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Primary Antibodies

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Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Assay Kits

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Abcam product promise