Anti-Angiotensin II Type 1 Receptor 抗体 (ab59018)
Key features and details
- Goat polyclonal to Angiotensin II Type 1 Receptor
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Angiotensin II Type 1 Receptor antibody
Angiotensin II Type 1 Receptor 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to Angiotensin II Type 1 Receptor -
由来種
Goat -
特異性
All reported variants represent identical protein (NP_000676.1, NP_004826.2, NP_033611.1, NP_114038.1 and NP_114438.1).
Preliminary testing was unsuccessful on Rat Liver in Western blot.
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アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Sheep, Rabbit, Guinea pig, Cow, Dog, Pig, Chimpanzee
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免疫原
Synthetic peptide:
C-EIQKNKPRNDDIFK
, corresponding to internal sequence amino acids 227-240 of Human Angiotensin II Type 1 Receptor -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab59018の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 41 kDa).
1 hour primary incubation is recommended for this product. |
| 特記事項 |
|---|
|
WB
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 41 kDa). 1 hour primary incubation is recommended for this product. |
ターゲット情報
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機能
Receptor for angiotensin II. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. -
組織特異性
Liver, lung, adrenal and adrenocortical adenomas. -
関連疾患
Defects in AGTR1 are a cause of renal tubular dysgenesis (RTD) [MIM:267430]. RTD is an autosomal recessive severe disorder of renal tubular development characterized by persistent fetal anuria and perinatal death, probably due to pulmonary hypoplasia from early-onset oligohydramnios (the Potter phenotype). -
配列類似性
Belongs to the G-protein coupled receptor 1 family. -
翻訳後修飾
C-terminal Ser or Thr residues may be phosphorylated. -
細胞内局在
Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 281607 Cow
- Entrez Gene: 185 Human
- Entrez Gene: 11607 Mouse
- Entrez Gene: 11608 Mouse
- Entrez Gene: 24180 Rat
- Entrez Gene: 81638 Rat
- Omim: 106165 Human
- SwissProt: P30556 Human
see all -
別名
- AG2S antibody
- Agtr 1 antibody
- Agtr1 antibody
see all
画像
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Western blot - Anti-Angiotensin II Type 1 Receptor antibody (ab59018)All lanes : Anti-Angiotensin II Type 1 Receptor antibody (ab59018) at 0.2 µg/ml
Lane 1 : Human liver lysate
Lane 2 : Human liver lysate with Immunizing peptide
Lane 3 : Mouse liver lysate
Lane 4 : Mouse liver lysate with Immunizing peptide
Lysates/proteins at 35 µg per lane.
Predicted band size: 41 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted?
Additional bands at: 45, 75 kDa. We are unsure as to the identity of these extra bands.Primary incubation: 1 hour at room temperature.
Detected by chemiluminescence.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab59018 は 5 報の論文で使用されています。
- Tang JM et al. Response Gene to Complement 32 Maintains Blood Pressure Homeostasis by Regulating a-Adrenergic Receptor Expression. Circ Res 123:1080-1090 (2018). PubMed: 30355157
- Goyal R et al. Antenatal maternal low protein diet: ACE-2 in the mouse lung and sexually dimorphic programming of hypertension. BMC Physiol 15:2 (2015). PubMed: 25971747
- Goyal R et al. Antenatal maternal protein deprivation: sexually dimorphic programming of the pancreatic renin-angiotensin system. J Renin Angiotensin Aldosterone Syst 14:137-45 (2013). PubMed: 22898440
- Goyal R et al. Antenatal maternal hypoxic stress: adaptations in fetal lung Renin-Angiotensin system. Reprod Sci 18:180-9 (2011). PubMed: 20978179
- Goyal R et al. Antenatal maternal hypoxic stress: adaptations of the placental renin-angiotensin system in the mouse. Placenta 32:134-9 (2011). PubMed: 21130492