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Anti-Angiopoietin 2/ANG2 抗体 [MM0020-1F29] (ab56301)

Submit a review Q&A (3)References (8)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Angiopoietin 2/ANG2 antibody [MM0020-1F29] (ab56301)
  • Western blot - Anti-Angiopoietin 2/ANG2 antibody [MM0020-1F29] (ab56301)

Key features and details

  • Mouse monoclonal [MM0020-1F29] to Angiopoietin 2/ANG2
  • Suitable for: IHC-P, WB
  • Reacts with: Human
  • Isotype: IgG2

製品の概要

  • 製品名

    Anti-Angiopoietin 2/ANG2 antibody [MM0020-1F29]
    Angiopoietin 2/ANG2 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [MM0020-1F29] to Angiopoietin 2/ANG2

  • 由来種

    Mouse

  • 特異性

    This antibody recognizes Angiopoietin 2/ANG2 in western blot without cross reactivity with Angiopoietin 1.

  • アプリケーション

    適用あり: IHC-P, WBmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Recombinant full length protein corresponding to Human Angiopoietin 2/ANG2.

  • ポジティブ･コントロール

    • Human endothelial cell lysate.

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • バッファー

    Constituent: PBS
  • Concentration information loading...

  • 精製度

    Protein G purified

  • 特記事項（精製）

    The IgG fraction of the tissue culture supernatant was purified and then lyophilized from 0.2 µm filtered PBS solution.

  • ポリ/モノ

    モノクローナル

  • クローン名

    MM0020-1F29

  • アイソタイプ

    IgG2

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab56301の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P
1/100 - 1/200.
WB
1/400 - 1/1000. Detects a band of approximately 54 kDa (predicted molecular weight: 57 kDa).
特記事項
IHC-P
1/100 - 1/200.
WB
1/400 - 1/1000. Detects a band of approximately 54 kDa (predicted molecular weight: 57 kDa).

ターゲット情報

  • 機能

    Can induce tyrosine phosphorylation of TIE2. Binds to TIE2 receptor and counteracts blood vessel maturation/stability mediated by angiopoietin-1. Its function may be context-dependent. In the absence of angiogenic inducers, such as VEGF, ANG2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal.

  • 配列類似性

    Contains 1 fibrinogen C-terminal domain.

  • ドメイン

    The Fibrinogen C-terminal domain mediates interaction with the TEK/TIE2 receptor.

  • 細胞内局在

    Secreted.
  • Information by UniProt

  • 参照データベース

    • 別名

      • AGPT 2 antibody
      • Agpt2 antibody
      • ANG 2 antibody
      • ANG-2 antibody
      • ANG2 antibody
      • Angiopoietin 2a antibody
      • Angiopoietin 2B antibody
      • Angiopoietin-2 antibody
      • Angiopoietin2 antibody
      • ANGP2_HUMAN antibody
      • ANGPT 2 antibody
      • Angpt2 antibody
      • Tie2 ligand antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Angiopoietin 2/ANG2 antibody [MM0020-1F29] (ab56301)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Angiopoietin 2/ANG2 antibody [MM0020-1F29] (ab56301)

      ab56301 at 1/200 dilution, staining Angiopoietin 2/ANG2 in human placental tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections).

    • Western blot - Anti-Angiopoietin 2/ANG2 antibody [MM0020-1F29] (ab56301)
      Western blot - Anti-Angiopoietin 2/ANG2 antibody [MM0020-1F29] (ab56301)
      Anti-Angiopoietin 2/ANG2 antibody [MM0020-1F29] (ab56301) at 1 µg/ml + Human liver tissue lysate - total protein (ab29889) at 10 µg

      Secondary
      Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 57 kDa
      Observed band size: 52 kDa why is the actual band size different from the predicted?
      Additional bands at: 47 kDa, 65 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 4 minutes


      The band observed at 52 kDa could potentially be a cleaved form of Angiopoietin 2/ANG2 due to the presence of an 18 amino acid signal peptide.

    参考文献 (8)

    ab56301 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab56301 は 8 報の論文で使用されています。

    • Wilson RC  et al. Utilizing Contrast-Enhanced Ultrasonography with Phosphatidylserine Microbubbles to Detect Placental Inflammation in Rhesus Macaques. Molecules 28:N/A (2023). PubMed: 37049657
    • Harbuzariu A  et al. Neuregulin-1/ErbB4 signaling modulates Plasmodium falciparum HRP2-induced damage to brain cortical organoids. iScience 25:104407 (2022). PubMed: 35663028
    • Diao H  et al. miR-135a-5p inhibits tumor invasion by targeting ANGPT2 in gallbladder cancer. Mol Med Rep 24:N/A (2021). PubMed: 34036386
    • Kim YJ  et al. Suppression of breast cancer progression by FBXL16 via oxygen-independent regulation of HIF1α stability. Cell Rep 37:109996 (2021). PubMed: 34818544
    • Guo W  et al. Analysis of vascular-associated factors and the prognosis of poorly differentiated hypopharyngeal carcinoma. Oncol Lett 20:271 (2020). PubMed: 32989405
    • Zhou CF  et al. miR-205-5p inhibits human endometriosis progression by targeting ANGPT2 in endometrial stromal cells. Stem Cell Res Ther 10:287 (2019). PubMed: 31547870
    • Lee YJ  et al. Hyperhomocysteinemia Causes Chorioretinal Angiogenesis with Placental Growth Factor Upregulation. Sci Rep 8:15755 (2018). PubMed: 30361676
    • Lee YJ  et al. Homocysteine Facilitates Prominent Polygonal Angiogenetic Networks of a Choroidal Capillary Sprouting Model. Invest Ophthalmol Vis Sci 58:4332–4343 (2017). PubMed: 28806447

    レビューと Q&A

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    レビューを送る 質問を送る

    1-3 of 3 Abreviews or Q&A

    Question

    I'm using this antibody to stain human placenta by IHC-P and just seeing high background.

    Read More
    Answer

    Thank you for contacting us.

    I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to contact our accounting department so that we may gather the correct information needed for the refund. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used.

    Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department.

    The credit note ID is for your reference only and does not automatically guarantee the credit.

    I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.

    Read More

    Question

    Thank you for the speedy response! I have looked at the two antibodies
    and have decided on ab56301, mainly for the lack of cross reactivity
    with Ang-1.

    Thank you very much for your help! I can't wait to get it!

    Read More
    Answer

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.
    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number xxx with ab56301. The item will be shipped on Monday for Tuesday delivery.
    To check the status of the order please contact our Customer Service team and reference this number.
    Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.
    I wish you the best of luck with your research.

    Read More

    Question

    Thank you for getting back to me, and for the good news!
    The most important thing about the Ang-2 antibody that I need is no,
    to VERY little cross reactivity with Ang-1. I am doing studies with
    Ang-2 and Ang-1 and need to be able to distinguish them on separate
    blots. I am using human endothelial cells and the primary usage will
    be Western blots. I don't believe that I will be IP'ing, so
    that is not important.


    Sorry I did not have the ordering information. The number sounds about
    right with their numbering system, but I don't know if that is it
    for sure. I hope that the information provided helps!
    Please let me know if there is anything else needed! I would like to
    get this Ang-2 Antibody and put it to good use!

    Read More
    Answer

    Thank you for your reply.
    I have aligned the human angiopoietin 1 and 2 proteins and the overall sequence homology is only 61%.
    I would recommend ab64015, since the immunogen does not align well with Ang1 (63% homology)
    https://www.abcam.com/Angiopoietin-2-antibody-ab64015.html
    or ab56301 (https://www.abcam.com/Angiopoietin-2-antibody-MM0020-1F29-ab56301.html) as this antibody recognizes Angiopoietin 2 in western blot without cross reactivity with Angiopoietin 1.
    Please let me know which of the 2 antibodies you would prefer and I will arrange the shipment for you. The order I have is the correct one. Thank you for the additional order information.
    Also, if the shipping address has changed in the meantime (since August), please let me know the new address.
    I look forward to hear back from you.

    Read More

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