Anti-Angiopoietin 2/ANG2 抗体 (ab8452)
Key features and details
- Rabbit polyclonal to Angiopoietin 2/ANG2
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Angiopoietin 2/ANG2 antibody
Angiopoietin 2/ANG2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Angiopoietin 2/ANG2 -
由来種
Rabbit -
特異性
Angiopoietin 2 Antibody antiserum is directed against mouse angiopoietin-2 and shows no reactivity with angiopoietin-1 from mouse-derived proteins. This reagent cross-reacts with human angiopoietin-2. Partially cross-reactivity is noted with human angiopoietin-1.
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アプリケーション
適用あり: IHC-P, ICC/IF, WBmore details -
種交差性
交差種: Mouse, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.1% Sodium azide
Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution, 98.5% Whole serum -
Concentration information loading... -
精製度
Whole antiserum -
特記事項（精製）
Angiopoietin 2 Antibody antiserum is directed against mouse angiopoietin-2 and shows no reactivity with angiopoietin-1 from mouse-derived proteins. This product was prepared from monospecific antiserum by a delipidation and defibrination. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab8452の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | (1) |
1/200 - 1/500.
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| ICC/IF | (1) |
Use at an assay dependent concentration.
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| WB | (2) |
1/500 - 1/2000.
There is reaction with serum in the cell supernatants, which results in strong background and makes it difficult to see the angiopoietins in cell supernatants. However when precipitated (using soluble Tie2) the signals are very good and strong. |
| 特記事項 |
|---|
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IHC-P
1/200 - 1/500. |
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ICC/IF
Use at an assay dependent concentration. |
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WB
1/500 - 1/2000. There is reaction with serum in the cell supernatants, which results in strong background and makes it difficult to see the angiopoietins in cell supernatants. However when precipitated (using soluble Tie2) the signals are very good and strong. |
ターゲット情報
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機能
Can induce tyrosine phosphorylation of TIE2. Binds to TIE2 receptor and counteracts blood vessel maturation/stability mediated by angiopoietin-1. Its function may be context-dependent. In the absence of angiogenic inducers, such as VEGF, ANG2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal. -
配列類似性
Contains 1 fibrinogen C-terminal domain. -
ドメイン
The Fibrinogen C-terminal domain mediates interaction with the TEK/TIE2 receptor. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 285 Human
- Entrez Gene: 11601 Mouse
- Omim: 601922 Human
- SwissProt: O15123 Human
- SwissProt: O35608 Mouse
- Unigene: 583870 Human
- Unigene: 439874 Mouse
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別名
- AGPT 2 antibody
- Agpt2 antibody
- ANG 2 antibody
see all
画像
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Western blot - Anti-Angiopoietin 2/ANG2 antibody (ab8452)This image is courtesy of Marion Scharpfenecker 2002
Supernatants of mouse angiopoietin-expressing endothelial cells. Soluble Tie 2 was used to precipitate the angiopoietins to reduce background.
Lane 1 - mock
Lane 2 - mouse angiopoietin-2 (clone 2-9) expressing cells
Lane 3 - mouse angiopoietin-1 (clone 1-15) expressing cells
Lane 4 - mouse angiopoietin-1 (clone 1-8) expressing cells
Lane 5 - wt -
Immunocytochemistry/ Immunofluorescence - Anti-Angiopoietin 2/ANG2 antibody (ab8452)ICC/IF image of ab8452 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab8452, 1/200 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (38)
ab8452 は 38 報の論文で使用されています。
- Lee E et al. An agonistic anti-Tie2 antibody suppresses the normal-to-tumor vascular transition in the glioblastoma invasion zone. Exp Mol Med 55:470-484 (2023). PubMed: 36828931
- Malespín-Bendaña W et al. Helicobacter pylori infection induces gastric precancerous lesions and persistent expression of Angpt2, Vegf-A and Tnf-A in a mouse model. Front Oncol 13:1072802 (2023). PubMed: 36874142
- Yu X et al. Depleted Long Noncoding RNA GAS5 Relieves Intervertebral Disc Degeneration via microRNA-17-3p/Ang-2. Oxid Med Cell Longev 2022:1792412 (2022). PubMed: 35340210
- Zhou HJ et al. Caveolae-mediated Tie2 signaling contributes to CCM pathogenesis in a brain endothelial cell-specific Pdcd10-deficient mouse model. Nat Commun 12:504 (2021). PubMed: 33495460
- Zhao Y et al. Activated mesangial cells induce glomerular endothelial cells proliferation in rat anti-Thy-1 nephritis through VEGFA/VEGFR2 and Angpt2/Tie2 pathway. Cell Prolif 54:e13055 (2021). PubMed: 33987885