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AB259823

Anti-Angiopoietin 2/ANG2 抗体 [EPR24161-73]

Anti-Angiopoietin 2/ANG2 antibody [EPR24161-73]

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Rabbit Recombinant Monoclonal Angiopoietin 2/ANG2 antibody. Suitable for WB and reacts with Mouse, Rat, Human samples.

別名を表示する

Angiopoietin-2, ANG-2, ANGPT2

4 Images
Western blot - Anti-Angiopoietin 2/ANG2 antibody [EPR24161-73] (AB259823)
  • WB

Lab

Western blot - Anti-Angiopoietin 2/ANG2 antibody [EPR24161-73] (AB259823)

Blocking and diluting buffer and concentration : 5% NFDM/TBSTThe molecular weight observed is consistent with what has been described in the literature (PMID : 29118335, PMID : 32908006)

Positive tissue : placenta (PMID : 9204896)

Exposure time : 48 seconds

All lanes:

Western blot - Anti-Angiopoietin 2/ANG2 antibody [EPR24161-73] (ab259823) at 1/1000 dilution

All lanes:

Human placenta tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 56 kDa

Observed band size: 60-75 kDa

false

Western blot - Anti-Angiopoietin 2/ANG2 antibody [EPR24161-73] (AB259823)
  • WB

Lab

Western blot - Anti-Angiopoietin 2/ANG2 antibody [EPR24161-73] (AB259823)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

The molecular weight observed is consistent with what has been described in the literature (PMID : 29118335, PMID : 32908006)

Positive cell line : HUVEC (PMID : 25857554)

Negative control : HEK-293 (PMID : 32908006)

Exposure time : Lanes 1-2 : 3 minutes; Lanes 3-4 : 81 seconds.

All lanes:

Western blot - Anti-Angiopoietin 2/ANG2 antibody [EPR24161-73] (ab259823) at 1/1000 dilution

Lane 1:

HUVEC (human umbilical vein endothelial cell) whole cell lysate at 20 µg

Lane 2:

HEK-293 (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Lane 3:

TF-1 (human erythroleukemia erythroblast) whole cell lysate at 20 µg

Lane 4:

TF-1 treated with PNGase F whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 56 kDa

Observed band size: 60-75 kDa

false

Western blot - Anti-Angiopoietin 2/ANG2 antibody [EPR24161-73] (AB259823)
  • WB

Lab

Western blot - Anti-Angiopoietin 2/ANG2 antibody [EPR24161-73] (AB259823)

Blocking and diluting buffer and concentration : 5% NFDM/TBST The molecular weight observed is consistent with what has been described in the literature (PMID : 29118335, PMID : 32908006) Positive tissue : placenta, ovary (PMID : 9204896) Exposure time : 3 minutes

All lanes:

Western blot - Anti-Angiopoietin 2/ANG2 antibody [EPR24161-73] (ab259823) at 1/1000 dilution

Lane 1:

Mouse placenta tissue lysate at 20 µg

Lane 2:

Mouse ovary tissue lysate at 20 µg

Lane 3:

Mouse pancreas tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 56 kDa

Observed band size: 60-75 kDa

false

Western blot - Anti-Angiopoietin 2/ANG2 antibody [EPR24161-73] (AB259823)
  • WB

Lab

Western blot - Anti-Angiopoietin 2/ANG2 antibody [EPR24161-73] (AB259823)

Blocking and diluting buffer and concentration : 5% NFDM/TBST The molecular weight observed is consistent with what has been described in the literature (PMID : 29118335, PMID : 32908006) Positive tissue : placenta, ovary (PMID : 9204896) Negative control : skeletal muscle (PMID : 9204896). Exposure time : 114 seconds

All lanes:

Western blot - Anti-Angiopoietin 2/ANG2 antibody [EPR24161-73] (ab259823) at 1/1000 dilution

Lane 1:

Rat ovary tissue lysate at 20 µg

Lane 2:

Rat placenta tissue lysate at 20 µg

Lane 3:

Rat skeletal muscle tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 56 kDa

Observed band size: 60-75 kDa

false

関連する標識済み抗体及び組成の異なる製品 (1)

  • Carrier free

    Anti-Angiopoietin 2/ANG2 antibody [EPR24161-73] - BSA and Azide free

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR24161-73

アイソタイプ

IgG

キャリアフリー

No

交差種

Mouse, Rat, Human

アプリケーション

WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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製品の詳細

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Angiopoietin-2 also known as ANG2 or ANGPT2 is a glycoprotein with a molecular mass of approximately 70 kDa. It plays an important role in angiogenesis facilitating blood vessel remodeling and maturation. The protein is mainly expressed in endothelial cells where it functions by binding to the Tie2 receptor partially disrupting the Angiopoietin-Tie2 signaling axis. This disruption modulates vascular stability and permeability. The lyophilized form of the ANG-2 protein is often used in research for functional assays like ANG-2 ELISA to quantify its levels in various biological samples.
Biological function summary

Human angiogenesis processes are significantly affected by Angiopoietin-2 which serves as an antagonist to Angiopoietin-1 in regulating vascular dynamics. Although it does not form a stable complex by itself ANG2 activity is closely linked to its interactions with the Tie2 receptor modulating signal pathways that govern endothelial cell survival and permeability. Because of its dual role either stabilizing or destabilizing blood vessels under different conditions ANG2 serves as an important regulator during both normal physiological events and pathophysiological conditions.

Pathways

Angiopoietin-2 significantly contributes to the angiogenesis signaling network. It is deeply involved in the VEGF (Vascular Endothelial Growth Factor) pathway where ANG2 acts to balance VEGF activities in angiogenesis and vessel permeability. The interplay between Angiopoietin-2 and other proteins such as Angiopoietin-1 which stabilizes the vessels is vital for the precise regulation of blood vessel homeostasis during normal and disturbed conditions.

Angiopoietin-2 has a prominent role in the pathology of diseases like cancer and diabetic retinopathy. In cancer excessive ANG2 expression may lead to increased tumor angiogenesis and metastasis making it a target for cancer therapies. In diabetic retinopathy elevated levels of ANG2 correlate with vascular leakage contributing to disease progression. The relationship between Angiopoietin-1 and Angiopoietin-2 in these conditions emphasizes their collective impact on vascular anomalies associated with these disorders.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Binds to TEK/TIE2, competing for the ANGPT1 binding site, and modulating ANGPT1 signaling (PubMed : 15284220, PubMed : 19116766, PubMed : 19223473, PubMed : 9204896). Can induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANGPT1 (PubMed : 15284220, PubMed : 19116766, PubMed : 19223473, PubMed : 9204896). In the absence of angiogenic inducers, such as VEGF, ANGPT2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal (PubMed : 15284220, PubMed : 19116766, PubMed : 19223473, PubMed : 9204896). Involved in the regulation of lymphangiogenesis (PubMed : 32908006).
See full target information ANGPT2
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組合せ製品

Assay Kits

AB316914

Human Angiopoietin 2/ANG2 ELISA Kit

SimpleStep

Sandwich ELISA - Human Angiopoietin 2/ANG2 ELISA Kit (AB316914)

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Secondary Antibodies

AB150077

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Primary Antibodies

AB9485

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Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

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Primary Antibodies

AB183701

Anti-Angiopoietin 1 antibody [EPR2888(N)]

RabMAb|Recombinant|20ul selling size

Western blot - Anti-Angiopoietin 1 antibody [EPR2888(N)] (AB183701)

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Abcam product promise

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