AB117776

Anti-Angiomotin 抗体

Name: Anti-Angiomotin 抗体 (ab117776) | Abcam
Brand: Abcam Limited
SKU: AB117776
Price: 76000 JPY
Availability: InStock

Anti-Angiomotin antibody

Be the first to review this product! Submit a review

(1 Publication)

Rabbit Polyclonal Angiomotin antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human AMOT aa 1000 to C-terminus.

別名を表示する

KIAA1071, AMOT, Angiomotin

2 Images

Unknown

Immunoprecipitation - Anti-Angiomotin antibody (AB117776)

Detection of Angiomotin by Western Blot of Immunprecipitate.
ab117776 at 1µg/ml staining Angiomotin in 293T whole cell lysate immunoprecipitated using ab117776 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection : Chemiluminescence with exposure time of 1 second.

All lanes:

Immunoprecipitation - Anti-Angiomotin antibody (ab117776)

Predicted band size: 118 kDa

false

Unknown

Western blot - Anti-Angiomotin antibody (AB117776)

All lanes:

Western blot - Anti-Angiomotin antibody (ab117776) at 0.04 µg/mL

Lane 1:

293T whole cell lysates at 50 µg

Lane 2:

293T whole cell lysates at 15 µg

Lane 3:

HeLa whole cell lysates at 50 µg

Predicted band size: 118 kDa

true

Exposure time: 3s

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

IP, WB

applications

免疫原

Synthetic Peptide within Human AMOT aa 1000 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q4VCS5

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Bat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Elephant": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

style

left-column

style

left-column

出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

精製に関する特記事項

ab117776 was affinity purified using an epitope specific to Angiomotin immobilized on solid support.

バッファー組成

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: 99% Tris buffered saline, 0.1% BSA

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

style

left-column

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Angiomotin also known as AMOT is a protein that has significant roles in various cellular processes. It has a molecular mass of approximately 130 kDa. Angiomotin is widely expressed across several tissues including endothelial cells where it functions as a regulator of cell migration and angiogenesis. It interacts directly with actin filaments affecting cell shape and movement therefore playing a role in the structural organization of cells.

Biological function summary

Angiomotin influences endothelial cell behavior through its involvement in cellular proliferation and mobility. Angiomotin forms part of a complex network by binding with other proteins such as Patj and LATS1/2 aiding in the control of the Hippo signaling pathway. This activity regulates organ size and cellular growth impacting tissue architecture and repair mechanisms in the body.

Pathways

Angiomotin's interactions contribute notably to the Hippo and MAPK signaling pathways. Within the Hippo pathway it aid the suppression of excessive cell proliferation by keeping the activity of YAP and TAZ in check. In the context of the MAPK pathway Angiomotin regulates signals that influence cell division and differentiation. The protein influences cellular outcomes by cooperating with other proteins like YAP a transcriptional coactivator often implicated in organ size and cancer.

Angiomotin is associated with various vascular conditions and cancers. Altered expression or dysregulation can contribute to pathological angiogenesis which plays a role in tumor progression and metastasis. In cancer Angiomotin impacts the Hippo pathway's ability to control YAP's oncogenic potential affecting cellular proliferation and tumor development. Understanding these interactions offers potential therapeutic targets for disorders linked to abnormal cell growth and vascular anomalies.

style

left-column

style

left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

ターゲットの情報

Plays a central role in tight junction maintenance via the complex formed with ARHGAP17, which acts by regulating the uptake of polarity proteins at tight junctions. Appears to regulate endothelial cell migration and tube formation. May also play a role in the assembly of endothelial cell-cell junctions. Repressor of YAP1 and WWTR1/TAZ transcription of target genes, potentially via regulation of Hippo signaling-mediated phosphorylation of YAP1 which results in its recruitment to tight junctions (PubMed : 21205866).

See full target information AMOT

style

left-column

文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Developmental cell 40:523-536.e6 PubMed28350986

2017

Flow-Dependent Endothelial YAP Regulation Contributes to Vessel Maintenance.

Applications

Unspecified application

Species

Unspecified reactive species

Hiroyuki Nakajima,Kimiko Yamamoto,Sobhika Agarwala,Kenta Terai,Hajime Fukui,Shigetomo Fukuhara,Koji Ando,Takahiro Miyazaki,Yasuhiro Yokota,Etienne Schmelzer,Heinz-Georg Belting,Markus Affolter,Virginie Lecaudey,Naoki Mochizuki

View all publications

style

left-column

style

left-column

style

right-column

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Angiomotin 抗体

Anti-Angiomotin antibody

Immunoprecipitation - Anti-Angiomotin antibody (AB117776)

Western blot - Anti-Angiomotin antibody (AB117776)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

精製に関する特記事項

バッファー組成

出荷温度

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (1)

代替製品

Primary Antibodies

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise