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リコンビナントRabMAb

Anti-Androgen Receptor 抗体 [SP107] - N-terminal (ab105225)

Reviews (8) Submit a question References (6)
Western blot - Anti-Androgen Receptor antibody [SP107] - N-terminal (ab105225)
  • Western blot - Anti-Androgen Receptor antibody [SP107] - N-terminal (ab105225)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - N-terminal (ab105225)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - N-terminal (ab105225)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - N-terminal (ab105225)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - N-terminal (ab105225)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - N-terminal (ab105225)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - N-terminal (ab105225)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - N-terminal (ab105225)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - N-terminal (ab105225)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - N-terminal (ab105225)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - N-terminal (ab105225)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - N-terminal (ab105225)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - N-terminal (ab105225)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - N-terminal (ab105225)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - N-terminal (ab105225)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - N-terminal (ab105225)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - N-terminal (ab105225)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - N-terminal (ab105225)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - N-terminal (ab105225)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - N-terminal (ab105225)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - N-terminal (ab105225)
  • Anti-Androgen Receptor antibody [SP107] - N-terminal (ab105225)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP107] to Androgen Receptor - N-terminal
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-Androgen Receptor antibody [SP107] - N-terminal
    Androgen Receptor 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [SP107] to Androgen Receptor - N-terminal

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: IHC-P, WBmore details

  • 種交差性

    交差種: Mouse, Rat, Human
    交差が予測される動物種: Dog, Pig

  • 免疫原

    Synthetic peptide within Human Androgen Receptor aa 300-400 (N terminal). The exact sequence is proprietary.
    Database link: P10275

  • ポジティブ･コントロール

    • IHC-P: Human prostate carcinoma, prostate carcinoma, breast ductal carcinoma, skin squamous cell carcinoma, uterus, endometrial carcinoma, breast, cervix, placenta, prostate, testis, bladder transitional cell carcinoma, hepatocellular carcinoma, lung carcinoma, lung squamous cell carcinoma, pancreatic carcinoma, ovarian carcinoma, Mouse testis, and Rat testis tissue. WB: Rat and Mouse testis tissue lysates; LnCAP and DU 145 cell lysates

  • 特記事項

    This product has switched from a hybridoma to recombinant production method on 23rd May 2023.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab105225の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P (2)
1/400. Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
WB (2)
Use a concentration of 1.55 µg/ml. Predicted molecular weight: 99 kDa.
特記事項
IHC-P
1/400. Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
WB
Use a concentration of 1.55 µg/ml. Predicted molecular weight: 99 kDa.

ターゲット情報

  • 機能

    Steroid hormone receptors are ligand-activated transcription factors that regulate eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Transcription factor activity is modulated by bound coactivator and corepressor proteins. Transcription activation is down-regulated by NR0B2. Activated, but not phosphorylated, by HIPK3 and ZIPK/DAPK3.
    Isoform 3 and isoform 4 lack the C-terminal ligand-binding domain and may therefore constitutively activate the transcription of a specific set of genes independently of steroid hormones.

  • 組織特異性

    Isoform 2 is mainly expressed in heart and skeletal muscle (PubMed:15634333). Isoform 3 is expressed by basal and stromal cells of prostate (at protein level) (PubMed:19244107).

  • 関連疾患

    Androgen insensitivity syndrome
    Spinal and bulbar muscular atrophy X-linked 1
    Defects in AR may play a role in metastatic prostate cancer. The mutated receptor stimulates prostate growth and metastases development despite of androgen ablation. This treatment can reduce primary and metastatic lesions probably by inducing apoptosis of tumor cells when they express the wild-type receptor.
    Androgen insensitivity, partial

  • 配列類似性

    Belongs to the nuclear hormone receptor family. NR3 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.

  • ドメイン

    Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain. In the presence of bound steroid the ligand-binding domain interacts with the N-terminal modulating domain, and thereby activates AR transcription factor activity. Agonist binding is required for dimerization and binding to target DNA. The transcription factor activity of the complex formed by ligand-activated AR and DNA is modulated by interactions with coactivator and corepressor proteins. Interaction with RANBP9 is mediated by both the N-terminal domain and the DNA-binding domain. Interaction with EFCAB6/DJBP is mediated by the DNA-binding domain.

  • 翻訳後修飾

    Sumoylated on Lys-388 (major) and Lys-521. Ubiquitinated. Deubiquitinated by USP26. 'Lys-6' and 'Lys-27'-linked polyubiquitination by RNF6 modulates AR transcriptional activity and specificity.
    Phosphorylated in prostate cancer cells in response to several growth factors including EGF. Phosphorylation is induced by c-Src kinase (CSK). Tyr-535 is one of the major phosphorylation sites and an increase in phosphorylation and Src kinase activity is associated with prostate cancer progression. Phosphorylation by TNK2 enhances the DNA-binding and transcriptional activity and may be responsible for androgen-independent progression of prostate cancer. Phosphorylation at Ser-83 by CDK9 regulates AR promoter selectivity and cell growth. Phosphorylation by PAK6 leads to AR-mediated transcription inhibition.
    Palmitoylated by ZDHHC7 and ZDHHC21. Palmitoylation is required for plasma membrane targeting and for rapid intracellular signaling via ERK and AKT kinases and cAMP generation.

  • 細胞内局在

    Nucleus. Cytoplasm. Predominantly cytoplasmic in unligated form but translocates to the nucleus upon ligand-binding. Can also translocate to the nucleus in unligated form in the presence of RACK1.
  • Information by UniProt

  • 参照データベース

    see all

  • 製品の状態

    There are 2 isoforms produced by alternative splicing. Isoform 1 is also known as: AR-B; isoform 2 is known as AR-A or variant AR45.

  • 別名

    • AIS antibody
    • ANDR_HUMAN antibody
    • Androgen nuclear receptor variant 2 antibody
    • Androgen receptor (dihydrotestosterone receptor; testicular feminization; spinal and bulbar muscular atrophy; Kennedy disease) antibody
    • Androgen receptor antibody
    • androgen receptor splice variant 4b antibody
    • AR antibody
    • AR8 antibody
    • DHTR antibody
    • Dihydro testosterone receptor antibody
    • Dihydrotestosterone receptor (DHTR) antibody
    • Dihydrotestosterone receptor antibody
    • HUMARA antibody
    • HYSP1 antibody
    • KD antibody
    • Kennedy disease (KD) antibody
    • NR3C4 antibody
    • Nuclear receptor subfamily 3 group C member 4 (NR3C4) antibody
    • Nuclear receptor subfamily 3 group C member 4 antibody
    • SBMA antibody
    • SMAX1 antibody
    • Spinal and bulbar muscular atrophy (SBMA) antibody
    • Spinal and bulbar muscular atrophy antibody
    • Testicular Feminization (TFM) antibody
    • TFM antibody
    see all

画像

  • Western blot - Anti-Androgen Receptor antibody [SP107] - N-terminal (ab105225)
    Western blot - Anti-Androgen Receptor antibody [SP107] - N-terminal (ab105225)
    All lanes : Anti-Androgen Receptor antibody [SP107] - N-terminal (ab105225) at 1/1000 dilution

    Lane 1 : Mouse liver tissue lysate
    Lane 2 : Rat liver tissue lysate
    Lane 3 : Mouse testis tissue lysate
    Lane 4 : Rat testis tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 99 kDa
    Observed band size: 110 kDa why is the actual band size different from the predicted?


    Exposure time: 60 seconds


    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    ab181602 was used as a GAPDH loading control.

  • Western blot - Anti-Androgen Receptor antibody [SP107] - N-terminal (ab105225)
    Western blot - Anti-Androgen Receptor antibody [SP107] - N-terminal (ab105225)
    All lanes : Anti-Androgen Receptor antibody [SP107] - N-terminal (ab105225) at 1.55 µg

    Lane 1 : LnCAP (Human prostate carcinoma epithelial cell) cell lysate
    Lane 2 : DU 145 (Human prostate carcinoma epithelial cell) cell lysate
    Lane 3 : Mouse testis tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 0.05 µg/ml

    Predicted band size: 99 kDa
    Observed band size: 120 kDa why is the actual band size different from the predicted?



    Blocking/Diluting buffer and concentration: 5% NFDM /TBST
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - N-terminal (ab105225)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - N-terminal (ab105225)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat testis tissue sections labeling Androgen Receptor with Purified ab105225 at 1/400 dilution (0.37 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 30mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - N-terminal (ab105225)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - N-terminal (ab105225)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse testis tissue sections labeling Androgen Receptor with Purified ab105225 at 1/400 dilution (0.37 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 30mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - N-terminal (ab105225)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - N-terminal (ab105225)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human prostate cancer tissue sections labeling Androgen Receptor with Purified ab105225 at 1/400 dilution (0.37 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 30mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - N-terminal (ab105225)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - N-terminal (ab105225)

    Formalin-fixed, paraffin-embedded human prostate carcinoma tissue stained for Androgen Receptor with ab105225 at 1/400 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - N-terminal (ab105225)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - N-terminal (ab105225)

    Formalin-fixed, paraffin-embedded human prostate adenocarcinoma tissue stained for Androgen Receptor with ab105225 at 1/400 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - N-terminal (ab105225)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - N-terminal (ab105225)

    Formalin-fixed, paraffin-embedded human breast ductal carcinoma tissue stained for Androgen Receptor with ab105225 at 1/400 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - N-terminal (ab105225)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - N-terminal (ab105225)

    Formalin-fixed, paraffin-embedded human skin squamous cell carcinoma tissue stained for Androgen Receptor with ab105225 at 1/400 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - N-terminal (ab105225)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - N-terminal (ab105225)

    Formalin-fixed, paraffin-embedded human uterus tissue stained for Androgen Receptor with ab105225 at 1/400 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - N-terminal (ab105225)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - N-terminal (ab105225)

    Formalin-fixed, paraffin-embedded human endometrial adenocarcinoma tissue stained for Androgen Receptor with ab105225 at 1/400 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - N-terminal (ab105225)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - N-terminal (ab105225)

    Formalin-fixed, paraffin-embedded human breast tissue stained for Androgen Receptor with ab105225 at 1/400 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - N-terminal (ab105225)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - N-terminal (ab105225)

    Formalin-fixed, paraffin-embedded human cervix tissue stained for Androgen Receptor with ab105225 at 1/400 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - N-terminal (ab105225)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - N-terminal (ab105225)

    Formalin-fixed, paraffin-embedded human placenta tissue stained for Androgen Receptor with ab105225 at 1/400 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - N-terminal (ab105225)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - N-terminal (ab105225)

    Formalin-fixed, paraffin-embedded human prostate tissue stained for Androgen Receptor with ab105225 at 1/400 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - N-terminal (ab105225)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - N-terminal (ab105225)

    Formalin-fixed, paraffin-embedded human testis tissue stained for Androgen Receptor with ab105225 at 1/400 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - N-terminal (ab105225)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - N-terminal (ab105225)

    Formalin-fixed, paraffin-embedded human bladder transitional cell carcinoma tissue stained for Androgen Receptor with ab105225 at 1/400 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - N-terminal (ab105225)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - N-terminal (ab105225)

    Formalin-fixed, paraffin-embedded human hepatocellular carcinoma tissue stained for Androgen Receptor with ab105225 at 1/400 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - N-terminal (ab105225)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - N-terminal (ab105225)

    Formalin-fixed, paraffin-embedded human lung adenocarcinoma tissue stained for Androgen Receptor with ab105225 at 1/400 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - N-terminal (ab105225)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - N-terminal (ab105225)

    Formalin-fixed, paraffin-embedded human lung squamous cell carcinoma tissue stained for Androgen Receptor with ab105225 at 1/400 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - N-terminal (ab105225)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - N-terminal (ab105225)

    Formalin-fixed, paraffin-embedded human pancreatic adenocarcinoma tissue stained for Androgen Receptor with ab105225 at 1/400 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - N-terminal (ab105225)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - N-terminal (ab105225)

    Formalin-fixed, paraffin-embedded human ovarian adenocarcinoma tissue stained for Androgen Receptor with ab105225 at 1/400 dilution in immunohistochemical analysis.

  • Anti-Androgen Receptor antibody [SP107] - N-terminal (ab105225)
    Anti-Androgen Receptor antibody [SP107] - N-terminal (ab105225)

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (6)

ab105225 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab105225 は 6 報の論文で使用されています。

  • Wang R  et al. PROTAC Degraders of Androgen Receptor-Integrated Dissolving Microneedles for Androgenetic Alopecia and Recrudescence Treatment via Single Topical Administration. Small Methods 7:e2201293 (2023). PubMed: 36538748
  • Matsumoto T  et al. Androgen promotes squamous differentiation of atypical cells in cervical intraepithelial neoplasia via an ELF3-dependent pathway. Cancer Med 12:10816-10828 (2023). PubMed: 36951594
  • Sakakibara I  et al. Myofiber androgen receptor increases muscle strength mediated by a skeletal muscle splicing variant of Mylk4. iScience 24:102303 (2021). PubMed: 33870126
  • Pareja F  et al. Immunohistochemical analysis of IDH2 R172 hotspot mutations in breast papillary neoplasms: applications in the diagnosis of tall cell carcinoma with reverse polarity. Mod Pathol 33:1056-1064 (2020). PubMed: 31896809
  • Beck ACC  et al. Salivary duct carcinoma: evaluation of treatment and outcome in a tertiary referral institute. Eur Arch Otorhinolaryngol 275:1885-1892 (2018). PubMed: 29785551
  • Aboukameel A  et al. Down-regulation of AR splice variants through XPO1 suppression contributes to the inhibition of prostate cancer progression. Oncotarget 9:35327-35342 (2018). PubMed: 30450161

レビューと Q&A

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1-8 of 8 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Androgen Receptor antibody [SP107] - N-terminal

 Excellent
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Mice prostate tissue)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris-EDTA buffer (pH 9.0)
Permeabilization
Yes - 0.2% Triton X-100 in PBS
Specification
Mice prostate tissue
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 37°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Armen Petrosyan

Verified customer

投稿 Dec 02 2022

Immunohistochemistry (Frozen sections) abreview for Anti-Androgen Receptor antibody [SP107] - N-terminal

 Excellent
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Testis)
Permeabilization
Yes - Triton X100
Specification
Testis
Blocking step
animal free blocker (Vector) as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 May 31 2022

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Androgen Receptor antibody [SP107] - N-terminal

 Good
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Human prostate tissue)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris-EDTA buffer (pH 9.0)
Permeabilization
Yes - 0.2% Triton X-100 in PBS
Specification
Human prostate tissue
Blocking step
Blocking according to ab64261 Rabbit specific HRP/DAB (ABC) Detection IHC Kit plus goat serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 37°C
Fixative
No fixation
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Armen Petrosyan

Verified customer

投稿 Aug 11 2021

ELISA abreview for Anti-Androgen Receptor antibody [SP107] - N-terminal

 Excellent
Abreviews
abreview image
Application
ELISA
Sample
Human Cell (HEK293T cells)
Specification
HEK293T cells
Blocking step
Superblock (Thermo Fisher) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Type
Sandwich (Detection)
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Jul 29 2021

Western blot abreview for Anti-Androgen Receptor antibody [SP107] - N-terminal

 Poor
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (MEFs)
Gel Running Conditions
Reduced Denaturing (4-15%)
Loading amount
25 µg
Specification
MEFs
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Mar 10 2021

Western blot abreview for Anti-Androgen Receptor antibody [SP107] - N-terminal

 Average
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (LNCaP cells)
Gel Running Conditions
Reduced Denaturing (4-15%)
Loading amount
25 µg
Specification
LNCaP cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Mar 05 2021

Immunocytochemistry/ Immunofluorescence abreview for Anti-Androgen Receptor antibody [SP107] - N-terminal

 Excellent
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Mice hepatocytes)
Permeabilization
Yes - 0.2% Triton X-100 in PBS
Specification
Mice hepatocytes
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde
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The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Armen Petrosyan

Verified customer

投稿 Jul 23 2020

Immunocytochemistry/ Immunofluorescence abreview for Anti-Androgen Receptor antibody [SP107] - N-terminal

 Excellent
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (LNCaP - prostate cancer cells)
Permeabilization
Yes - 0.2% Triton X-100 in PBS
Specification
LNCaP - prostate cancer cells
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Armen Petrosyan

Verified customer

投稿 Jul 02 2020

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