Name: Anti-Androgen Receptor antibody [EPR1535(2)]
Brand: RabMAb
SKU: ab133273
Rating: 5 (11 reviews)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [EPR1535(2)] (ab133273)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR1535(2)] to Androgen Receptor
Suitable for: WB, IHC-P, ICC/IF
Reacts with: Mouse, Rat, Human

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 647 Carrier Free HRP

製品名

Anti-Androgen Receptor antibody [EPR1535(2)]
Androgen Receptor 一次抗体製品一覧
製品の詳細

Rabbit monoclonal [EPR1535(2)] to Androgen Receptor
由来種

Rabbit
アプリケーション

適用あり: WB, IHC-P, ICC/IFmore details
種交差性

交差種: Mouse, Rat, Human
免疫原

Synthetic peptide within Human Androgen Receptor aa 1-100 (N terminal). The exact sequence is proprietary.
Database link: P10275
(Peptide available as ab191380)
ポジティブ･コントロール
- WB: T47-D, LnCaP and 22Rv1 cell lysates; Rat and mouse prostate lysates. IHC-P: Human prostate, prostatic adenocarcinoma, prostatic hyperplasia tissues; Rat and mouse testis tissues; Breast carcinoma and prostatic carcinoma T3 tissues. ICC/IF: MCF7 cells;EP156T-AR, 957E/hTERT-AR and LNCaP cells.
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

製品の状態

Liquid
保存方法

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
バッファー

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
Concentration information loading...
精製度

Protein A purified
ポリ/モノ

モノクローナル
クローン名

EPR1535(2)
アイソタイプ

IgG
研究分野
- Neuroscience
- Development
- Neuroscience
- Processes

Alternative Versions
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Positive Controls
- LNCaP nuclear extract lysate (ab14857)
Recombinant Protein
- Recombinant Human Androgen Receptor protein (His tag) (ab82124)
Related Products
- Prestained Protein Ladder - Broad molecular weight (10 - 245 kDa) (ab116028)

The Abpromise guarantee

Abpromise保証は、次のテスト済みアプリケーションにおけるab133273の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション	Abreviews	特記事項
WB	(2)	1/2000. Predicted molecular weight: 98 kDa. For unpurified use at 1:1000 For Lysate preparation protocol, please refer to the protocol book in the protocol section and/or here (downloadable copy).
IHC-P	(7)	1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. See IHC antigen retrieval protocols. For unpurified use at 1/100-1/250
ICC/IF	(1)	1/100 - 1/250.

特記事項
WB 1/2000. Predicted molecular weight: 98 kDa. For unpurified use at 1:1000 For Lysate preparation protocol, please refer to the protocol book in the protocol section and/or here (downloadable copy).
IHC-P 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. See IHC antigen retrieval protocols. For unpurified use at 1/100-1/250
ICC/IF 1/100 - 1/250.

機能

Steroid hormone receptors are ligand-activated transcription factors that regulate eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Transcription factor activity is modulated by bound coactivator and corepressor proteins. Transcription activation is down-regulated by NR0B2. Activated, but not phosphorylated, by HIPK3 and ZIPK/DAPK3.
Isoform 3 and isoform 4 lack the C-terminal ligand-binding domain and may therefore constitutively activate the transcription of a specific set of genes independently of steroid hormones.
組織特異性

Isoform 2 is mainly expressed in heart and skeletal muscle (PubMed:15634333). Isoform 3 is expressed by basal and stromal cells of prostate (at protein level) (PubMed:19244107).
関連疾患

Androgen insensitivity syndrome
Spinal and bulbar muscular atrophy X-linked 1
Defects in AR may play a role in metastatic prostate cancer. The mutated receptor stimulates prostate growth and metastases development despite of androgen ablation. This treatment can reduce primary and metastatic lesions probably by inducing apoptosis of tumor cells when they express the wild-type receptor.
Androgen insensitivity, partial
配列類似性

Belongs to the nuclear hormone receptor family. NR3 subfamily.
Contains 1 nuclear receptor DNA-binding domain.
ドメイン

Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain. In the presence of bound steroid the ligand-binding domain interacts with the N-terminal modulating domain, and thereby activates AR transcription factor activity. Agonist binding is required for dimerization and binding to target DNA. The transcription factor activity of the complex formed by ligand-activated AR and DNA is modulated by interactions with coactivator and corepressor proteins. Interaction with RANBP9 is mediated by both the N-terminal domain and the DNA-binding domain. Interaction with EFCAB6/DJBP is mediated by the DNA-binding domain.
翻訳後修飾

Sumoylated on Lys-388 (major) and Lys-521. Ubiquitinated. Deubiquitinated by USP26. 'Lys-6' and 'Lys-27'-linked polyubiquitination by RNF6 modulates AR transcriptional activity and specificity.
Phosphorylated in prostate cancer cells in response to several growth factors including EGF. Phosphorylation is induced by c-Src kinase (CSK). Tyr-535 is one of the major phosphorylation sites and an increase in phosphorylation and Src kinase activity is associated with prostate cancer progression. Phosphorylation by TNK2 enhances the DNA-binding and transcriptional activity and may be responsible for androgen-independent progression of prostate cancer. Phosphorylation at Ser-83 by CDK9 regulates AR promoter selectivity and cell growth. Phosphorylation by PAK6 leads to AR-mediated transcription inhibition.
Palmitoylated by ZDHHC7 and ZDHHC21. Palmitoylation is required for plasma membrane targeting and for rapid intracellular signaling via ERK and AKT kinases and cAMP generation.
細胞内局在

Nucleus. Cytoplasm. Predominantly cytoplasmic in unligated form but translocates to the nucleus upon ligand-binding. Can also translocate to the nucleus in unligated form in the presence of RACK1.
Target information above from: UniProt accession P10275 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
参照データベース
- Entrez Gene: 367 Human
- Entrez Gene: 11835 Mouse
- Entrez Gene: 24208 Rat
- Omim: 313700 Human
- SwissProt: P10275 Human
- SwissProt: P19091 Mouse
- SwissProt: P15207 Rat
- Unigene: 76704 Human
- Unigene: 39005 Mouse
- Unigene: 394224 Mouse
- Unigene: 439657 Mouse
- Unigene: 9813 Rat
see all
製品の状態

There are 2 isoforms produced by alternative splicing. Isoform 1 is also known as: AR-B; isoform 2 is known as AR-A or variant AR45.
別名
- AIS antibody
- ANDR_HUMAN antibody
- Androgen nuclear receptor variant 2 antibody
- Androgen receptor (dihydrotestosterone receptor; testicular feminization; spinal and bulbar muscular atrophy; Kennedy disease) antibody
- Androgen receptor antibody
- androgen receptor splice variant 4b antibody
- AR antibody
- AR8 antibody
- DHTR antibody
- Dihydro testosterone receptor antibody
- Dihydrotestosterone receptor (DHTR) antibody
- Dihydrotestosterone receptor antibody
- HUMARA antibody
- HYSP1 antibody
- KD antibody
- Kennedy disease (KD) antibody
- NR3C4 antibody
- Nuclear receptor subfamily 3 group C member 4 (NR3C4) antibody
- Nuclear receptor subfamily 3 group C member 4 antibody
- SBMA antibody
- SMAX1 antibody
- Spinal and bulbar muscular atrophy (SBMA) antibody
- Spinal and bulbar muscular atrophy antibody
- Testicular Feminization (TFM) antibody
- TFM antibody
see all

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [EPR1535(2)] (ab133273)

Immunohistochemical analysis of formalin fixed paraffin embedded human prostate cancer labelling Androgen Receptor with ab133273 at a concentration of 0.1µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with an OptiView DAB IHC Detection Kit. Heat mediated antigen retrieval was conducted for 32 mins at 100°C with ULTRA cell conditioning solution (CC1, pH 8.5). ab133273 Anti-Androgen Receptor antibody [EPR1535(2)] was incubated at 37°C for 16 mins. Sections were counterstained with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [EPR1535(2)] (ab133273)

Immunohistochemical analysis of formalin fixed paraffin embedded human prostate cancer labelling Androgen Receptor with ab133273 at a concentration of 0.1µg/ml. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with a Bond™ Polymer Refine Detection kit. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution 2) for 20 mins. ab133273 Anti-Androgen Receptor antibody [EPR1535(2)] was incubated for 30 mins at room temperature. Sections were counterstained with Hematoxylin. Image inset shows absence of staining in secondary antibody only control.
Western blot - Anti-Androgen Receptor antibody [EPR1535(2)] (ab133273)

All lanes : Anti-Androgen Receptor antibody [EPR1535(2)] (ab133273) at 1/1000 dilution

Lane 1 : Mouse testis lysate
Lane 2 : Rat testis lysate
Lane 3 : Mouse liver lysate
Lane 4 : Rat liver lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 98 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?

Exposure time: 20 seconds

Blocking/Diluting buffer: 5% NFDM/TBST

Loading Control: Rabbit monoclonal [EPR16891] to GAPDH (ab181602)
Western blot - Anti-Androgen Receptor antibody [EPR1535(2)] (ab133273)

Anti-Androgen Receptor antibody [EPR1535(2)] (ab133273) at 1/5000 dilution (purified) + Mouse prostate lysates at 15 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 98 kDa

Blocking and diluting buffer: 5% NFDM/TBST
Immunocytochemistry/ Immunofluorescence - Anti-Androgen Receptor antibody [EPR1535(2)] (ab133273)Image from Azeem W et al., PLoS One. 2017;12(6):e0177861. Fig 6.; doi: 10.1371/journal.pone.0177861. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

Texas red (Tx-Red) indirect immunofluorescent detection of Androgen Receptor using ab133273 in (A) EP156T-AR, (B) 957E/hTERT-AR and (C) LNCaP cells. The cells were treated with ± 1 nM R1881 for 24 hours.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [EPR1535(2)] (ab133273)shao et al Oncotarget. 2018 Mar 6; 9(18): 14456–14471. Published online 2018 Feb 12. doi: 10.18632/oncotarget.24470

Formalin-fixed, paraffin-embedded tissues labeling Androgen Receptor with ab133273. Left: Tumor from orthotopic injectionof PTEN KD/TE cells. Right: Human postate cancer tissue.

From Figure 3 of Shao et al/

Shao et al Oncotarget. 2018 Mar 6; 9(18): 14456–14471.Published online 2018 Feb 12. doi: 10.18632/oncotarget.24470

Reproduced under the terms of the Creative Commons Attribution License 3.0 (CC BY 3.0).
Immunocytochemistry/ Immunofluorescence - Anti-Androgen Receptor antibody [EPR1535(2)] (ab133273)

Immunocytochemistry/ Immunofluorescence analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling Androgen receptor with purified ab133273 at 1:100 dilution (1.3μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1:200 (2.5 μg/ml). ab150077 Goat anti rabbit IgG(Alexa Fluor^® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [EPR1535(2)] (ab133273)

Immunohistochemical analysis of paraffin-embedded human prostatic adenocarcinoma tissue labelling Androgen Receptor using unpurified ab133273 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Western blot - Anti-Androgen Receptor antibody [EPR1535(2)] (ab133273)

All lanes : Anti-Androgen Receptor antibody [EPR1535(2)] (ab133273) at 1/20000 dilution

Lane 1 : LNCaP (Human prostate carcinoma epithelial cell) whole cell lysates prepared in RIPA lysis method
Lane 2 : LNCaP (Human prostate carcinoma epithelial cell) whole cell lysates prepared in 1%SDS Hot lysis method
Lane 3 : 22Rv1 (Human prostate carcinoma epithelial cell) whole cell lysates prepared in RIPA lysis method
Lane 4 : 22Rv1 (Human prostate carcinoma epithelial cell) whole cell lysates prepared in 1%SDS Hot lysis method

Lysates/proteins at 1/15 dilution per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Predicted band size: 98 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?

Exposure time: 10 seconds

Blocking/Diluting buffer - 5% NFDM/TBST

The androgen receptor variant band detected in 22RV1 cells is reported by PMID: 22315407.

We recommend you to try both RIPA and 1%SDS Hot lysis preparation methods to get desired bands.
Western blot - Anti-Androgen Receptor antibody [EPR1535(2)] (ab133273)

Different batches of ab133273 were tested on LNCaP (Human prostate carcinoma epithelial cell) lysate at 1.0 µg/ml. 15 µg of lysate was loaded in each lane. Bands observed at 120 kDa.
Immunocytochemistry/ Immunofluorescence - Anti-Androgen Receptor antibody [EPR1535(2)] (ab133273)

Immunofluorescent staining of LnCAP cells labelling Androgen Receptor using unpurified ab133273, at 1/100 dilution
Western blot - Anti-Androgen Receptor antibody [EPR1535(2)] (ab133273)

All lanes : Anti-Androgen Receptor antibody [EPR1535(2)] (ab133273) at 1/5000 dilution (purified)

Lane 1 : LNCaP (Human prostate carcinoma epithelial cell) whole cell lysates
Lane 2 : Rat prostate lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

Predicted band size: 98 kDa

Blocking and diluting buffer: 5% NFDM/TBST
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [EPR1535(2)] (ab133273)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat testis tissue sections labeling Androgen Receptor with Purified ab133273 at 1:500 dilution (0.25 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [EPR1535(2)] (ab133273)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse testis tissue sections labeling Androgen Receptor with Purified ab133273 at 1:500 dilution (0.25 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [EPR1535(2)] (ab133273)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostatic hyperplasia tissue sections labeling Androgen Receptor with Purified ab133273 at 1:500 dilution (0.25 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
Western blot - Anti-Androgen Receptor antibody [EPR1535(2)] (ab133273)

Anti-Androgen Receptor antibody [EPR1535(2)] (ab133273) at 1/1000 dilution (unpurified) + LNCaP (Human prostate carcinoma epithelial cell) at 10 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 98 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?

Exposure time: 3 seconds

Blocking/Diluting buffer 5% NFDM/TBST
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [EPR1535(2)] (ab133273)

Immunohistochemical analysis of paraffin-embedded human prostate tissue labelling Androgen Receptor using unpurified ab133273, at1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [EPR1535(2)] (ab133273)

Unpurified ab133273 showing positive staining in Breast carcinoma tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [EPR1535(2)] (ab133273)

Unpurified ab133273 showing negative staining in Normal brain tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [EPR1535(2)] (ab133273)

Unpurified ab133273 showing negative staining in Normal tonsil tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [EPR1535(2)] (ab133273)

Unpurified ab133273 showing positive staining in Endometrial carcinoma tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [EPR1535(2)] (ab133273)

Unpurified ab133273 showing negative staining in Normal breast tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [EPR1535(2)] (ab133273)

Unpurified ab133273 showing negative staining in Normal colon tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [EPR1535(2)] (ab133273)

Unpurified ab133273 showing negative staining in Ovarian carcinoma tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [EPR1535(2)] (ab133273)

Unpurified ab133273 showing negative staining in Colonic adenocarcinoma tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [EPR1535(2)] (ab133273)

Unpurified ab133273 showing negative staining in Normal liver tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [EPR1535(2)] (ab133273)

Unpurified ab133273 showing positive staining in Prostatic carcinoma T3 tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-Androgen Receptor antibody [EPR1535(2)] (ab133273)

Western blot protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Protocol Booklet

Click here to view the general protocols

SDS download

Country/Region

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Datasheet download

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ab133273 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab133273 は 124 報の論文で使用されています。

Galbiati M et al. Bicalutamide and Trehalose Ameliorate Spinal and Bulbar Muscular Atrophy Pathology in Mice. Neurotherapeutics 20:524-545 (2023). PubMed: 36717478
Soave C et al. The Small Molecule Antagonist KCI807 Disrupts Association of the Amino-Terminal Domain of the Androgen Receptor with ELK1 by Modulating the Adjacent DNA Binding Domain. Mol Pharmacol 103:211-220 (2023). PubMed: 36720643
Dhital B et al. Harnessing transcriptionally driven chromosomal instability adaptation to target therapy-refractory lethal prostate cancer. Cell Rep Med 4:100937 (2023). PubMed: 36787737
Nätkin R et al. Adaptive and non-adaptive gene expression responses in prostate cancer during androgen deprivation. PLoS One 18:e0281645 (2023). PubMed: 36809527
Zhang Z et al. Efficacy and mechanism of lipoic acid in the treatment of reproductive injury caused by perfluorooctanoic acid. Exp Ther Med 25:116 (2023). PubMed: 36815965

View all Publications for this product

レビューと Q&A

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レビューを送る質問を送る

1-10 of 13 Abreviews or Q&A

Application

Immunocytochemistry/ Immunofluorescence

Sample

Mouse Cell (Naive Mouse Prostate)

Permeabilization

Specification

Naive Mouse Prostate

Blocking step

Dako Protein block serum-free as blocking agent for 3 hour(s) and 0 minute(s) · Temperature: 21°C

Fixative

Acetone

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Kianoosh Mempel

Verified customer

投稿 Jun 29 2023

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Dog Tissue sections (Ovary, Adenocarcinoma)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Sodium citrate buffer, pH6

Permeabilization

Specification

Ovary, Adenocarcinoma

Blocking step

Serum as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 3% · Temperature: 25°C

Fixative

Formaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Oct 12 2023

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Guinea pig Tissue sections (prostate)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: 1mM EDTA pH8.0

Permeabilization

Specification

prostate

Blocking step

Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

Fixative

Formaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

PROF. 清和尾崎

Verified customer

投稿 Jun 27 2022

Application

ELISA

Sample

Human Cell (HEK293T cells)

Specification

HEK293T cells

Blocking step

Superblock (Thermo Fisher) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C

Type

Sandwich (Detection)

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Jul 28 2021

Application

Western blot

Sample

Human Cell lysate - whole cell (LNCaP cells)

Gel Running Conditions

Reduced Denaturing

Loading amount

25 µg

Specification

LNCaP cells

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Mar 05 2021

Application

Western blot

Sample

Mouse Cell lysate - whole cell (MEFs)

Gel Running Conditions

Reduced Denaturing (4-15%)

Loading amount

25 µg

Specification

MEFs

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Mar 05 2021

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Human Tissue sections (Prostate)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Dako Flex TRS High pH 9

Specification

Prostate

Fixative

Formaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Mark Butters

Verified customer

投稿 Feb 10 2021

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Mouse Tissue sections (Testis)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH9

Permeabilization

Specification

Testis

Blocking step

Serum as blocking agent for 20 minute(s) · Concentration: 2.5% · Temperature: 23°C

Fixative

Formaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Victor Bernal

Verified customer

投稿 Mar 20 2020

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Human Tissue sections (prostate cancer)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: 10mM Sodium Citrate pH6

Permeabilization

Specification

prostate cancer

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Fixative

10% normal buffered formalin

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 May 21 2019

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Mouse Tissue sections (prostate)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: 10mM Citrate pH6

Permeabilization

Specification

prostate

Blocking step

Serum as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Fixative

10% formalin

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投稿 May 21 2019

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参考文献 (124)

レビューと Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-Androgen Receptor antibody [EPR1535(2)]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Androgen Receptor antibody [EPR1535(2)]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Androgen Receptor antibody [EPR1535(2)]

ELISA abreview for Anti-Androgen Receptor antibody [EPR1535(2)]

Western blot abreview for Anti-Androgen Receptor antibody [EPR1535(2)]

Western blot abreview for Anti-Androgen Receptor antibody [EPR1535(2)]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Androgen Receptor antibody [EPR1535(2)]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Androgen Receptor antibody [EPR1535(2)]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Androgen Receptor antibody [EPR1535(2)]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Androgen Receptor antibody [EPR1535(2)]