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  1. Link

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Signal Transduction Signaling Pathway Nuclear Signaling Nuclear Hormone Receptors Testosterone
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リコンビナントRabMAb

Anti-Androgen Receptor 抗体 [EP670Y] (ab52615)

  • Datasheet
  • SDS
Reviews (5)Q&A (5)References (29)

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Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Western blot - Anti-Androgen Receptor antibody [EP670Y] (ab52615)
  • Western blot - Anti-Androgen Receptor antibody [EP670Y] (ab52615)
  • Western blot - Anti-Androgen Receptor antibody [EP670Y] (ab52615)
  • Western blot - Anti-Androgen Receptor antibody [EP670Y] (ab52615)
  • Western blot - Anti-Androgen Receptor antibody [EP670Y] (ab52615)
  • Anti-Androgen Receptor antibody [EP670Y] (ab52615)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP670Y] to Androgen Receptor
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

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関連製品

製品の概要

  • 製品名

    Anti-Androgen Receptor antibody [EP670Y]
    Androgen Receptor 一次抗体 製品一覧
  • 製品の詳細

    Rabbit monoclonal [EP670Y] to Androgen Receptor
  • 由来種

    Rabbit
  • アプリケーション

    適用あり: WBmore details
    適用なし: Flow Cyt,ICC/IF or IHC-P
  • 種交差性

    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide within Human Androgen Receptor aa 900 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: P10275
    (Peptide available as ab174742)

  • ポジティブ・コントロール

    • WB: LnCaP and 22Rv1 cell lysate and mouse and rat testis tissue lysates.
  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • バッファー

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • 精製度

    Protein A purified
  • ポリ/モノ

    モノクローナル
  • クローン名

    EP670Y
  • アイソタイプ

    IgG
  • 研究分野

    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • Nuclear Hormone Receptors
    • Testosterone
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • Nuclear Receptors
    • Testosterone
    • Cancer
    • Signal transduction
    • Nuclear signaling
    • Nuclear hormone receptors
    • Other
    • Developmental Biology
    • Reproduction
    • Hormones
    • Neuroscience
    • Development
    • Neuroscience
    • Processes

関連製品

  • Alternative Versions

    • Anti-Androgen Receptor antibody [EP670Y] - BSA and Azide free (ab208088)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (ab96899)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human Androgen Receptor protein (His tag) (ab82124)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab52615の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (2)
1/1000 - 1/10000. Predicted molecular weight: 99 kDa.

We recommend to use 1%SDS Hot lysis prepare method.

特記事項
WB
1/1000 - 1/10000. Predicted molecular weight: 99 kDa.

We recommend to use 1%SDS Hot lysis prepare method.

追加情報
Is unsuitable for Flow Cyt,ICC/IF or IHC-P.

ターゲット情報

  • 機能

    Steroid hormone receptors are ligand-activated transcription factors that regulate eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Transcription factor activity is modulated by bound coactivator and corepressor proteins. Transcription activation is down-regulated by NR0B2. Activated, but not phosphorylated, by HIPK3 and ZIPK/DAPK3.
    Isoform 3 and isoform 4 lack the C-terminal ligand-binding domain and may therefore constitutively activate the transcription of a specific set of genes independently of steroid hormones.
  • 組織特異性

    Isoform 2 is mainly expressed in heart and skeletal muscle (PubMed:15634333). Isoform 3 is expressed by basal and stromal cells of prostate (at protein level) (PubMed:19244107).
  • 関連疾患

    Androgen insensitivity syndrome
    Spinal and bulbar muscular atrophy X-linked 1
    Defects in AR may play a role in metastatic prostate cancer. The mutated receptor stimulates prostate growth and metastases development despite of androgen ablation. This treatment can reduce primary and metastatic lesions probably by inducing apoptosis of tumor cells when they express the wild-type receptor.
    Androgen insensitivity, partial
  • 配列類似性

    Belongs to the nuclear hormone receptor family. NR3 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • ドメイン

    Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain. In the presence of bound steroid the ligand-binding domain interacts with the N-terminal modulating domain, and thereby activates AR transcription factor activity. Agonist binding is required for dimerization and binding to target DNA. The transcription factor activity of the complex formed by ligand-activated AR and DNA is modulated by interactions with coactivator and corepressor proteins. Interaction with RANBP9 is mediated by both the N-terminal domain and the DNA-binding domain. Interaction with EFCAB6/DJBP is mediated by the DNA-binding domain.
  • 翻訳後修飾

    Sumoylated on Lys-388 (major) and Lys-521. Ubiquitinated. Deubiquitinated by USP26. 'Lys-6' and 'Lys-27'-linked polyubiquitination by RNF6 modulates AR transcriptional activity and specificity.
    Phosphorylated in prostate cancer cells in response to several growth factors including EGF. Phosphorylation is induced by c-Src kinase (CSK). Tyr-535 is one of the major phosphorylation sites and an increase in phosphorylation and Src kinase activity is associated with prostate cancer progression. Phosphorylation by TNK2 enhances the DNA-binding and transcriptional activity and may be responsible for androgen-independent progression of prostate cancer. Phosphorylation at Ser-83 by CDK9 regulates AR promoter selectivity and cell growth. Phosphorylation by PAK6 leads to AR-mediated transcription inhibition.
    Palmitoylated by ZDHHC7 and ZDHHC21. Palmitoylation is required for plasma membrane targeting and for rapid intracellular signaling via ERK and AKT kinases and cAMP generation.
  • 細胞内局在

    Nucleus. Cytoplasm. Predominantly cytoplasmic in unligated form but translocates to the nucleus upon ligand-binding. Can also translocate to the nucleus in unligated form in the presence of RACK1.
  • Target information above from: UniProt accession P10275 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 367 Human
    • Entrez Gene: 11835 Mouse
    • Entrez Gene: 24208 Rat
    • Omim: 313700 Human
    • SwissProt: P10275 Human
    • SwissProt: P19091 Mouse
    • SwissProt: P15207 Rat
    • Unigene: 76704 Human
    • Unigene: 39005 Mouse
    • Unigene: 394224 Mouse
    • Unigene: 439657 Mouse
    • Unigene: 9813 Rat
    see all
  • 製品の状態

    There are 2 isoforms produced by alternative splicing. Isoform 1 is also known as: AR-B; isoform 2 is known as AR-A or variant AR45.
  • 別名

    • AIS antibody
    • ANDR_HUMAN antibody
    • Androgen nuclear receptor variant 2 antibody
    • Androgen receptor (dihydrotestosterone receptor; testicular feminization; spinal and bulbar muscular atrophy; Kennedy disease) antibody
    • Androgen receptor antibody
    • androgen receptor splice variant 4b antibody
    • AR antibody
    • AR8 antibody
    • DHTR antibody
    • Dihydro testosterone receptor antibody
    • Dihydrotestosterone receptor (DHTR) antibody
    • Dihydrotestosterone receptor antibody
    • HUMARA antibody
    • HYSP1 antibody
    • KD antibody
    • Kennedy disease (KD) antibody
    • NR3C4 antibody
    • Nuclear receptor subfamily 3 group C member 4 (NR3C4) antibody
    • Nuclear receptor subfamily 3 group C member 4 antibody
    • SBMA antibody
    • SMAX1 antibody
    • Spinal and bulbar muscular atrophy (SBMA) antibody
    • Spinal and bulbar muscular atrophy antibody
    • Testicular Feminization (TFM) antibody
    • TFM antibody
    see all

画像

  • Western blot - Anti-Androgen Receptor antibody [EP670Y] (ab52615)
    Western blot - Anti-Androgen Receptor antibody [EP670Y] (ab52615)
    All lanes : Anti-Androgen Receptor antibody [EP670Y] (ab52615) at 1/1000 dilution

    Lane 1 : Mouse testis lysate
    Lane 2 : Rat testis lysate
    Lane 3 : Mouse liver lysate
    Lane 4 : Rat liver lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 99 kDa
    Observed band size: 110 kDa why is the actual band size different from the predicted?


    Exposure time: 1 minute


    Blocking/Diluting buffer: 5% NFDM/TBST

    Loading control: Rabbit monoclonal [EPR16891] to GAPDH (ab181602)

  • Western blot - Anti-Androgen Receptor antibody [EP670Y] (ab52615)
    Western blot - Anti-Androgen Receptor antibody [EP670Y] (ab52615)
    All lanes : Anti-Androgen Receptor antibody [EP670Y] (ab52615) at 1/1000 dilution

    Lane 1 : LnCAP whole cell lysate (positive control)
    Lane 2 : PC3 whole cell lysate (negative control)

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 99 kDa
    Observed band size: 120 kDa why is the actual band size different from the predicted?
    Additional bands at: 58 kDa. We are unsure as to the identity of these extra bands.



    Lanes 1 - 2: Merged signal (red and green). Green - ab52615 observed at 120 kDa. Red - loading control, ab8245, observed at 37 kDa.

     

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab52615 and ab8245 (loading control) overnight at 4°C. Antibody binding was detected using Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at a 1:10000 dilution for 1hr at room temperature and then imaged.

  • Western blot - Anti-Androgen Receptor antibody [EP670Y] (ab52615)
    Western blot - Anti-Androgen Receptor antibody [EP670Y] (ab52615)
    All lanes : Anti-Androgen Receptor antibody [EP670Y] (ab52615) at 1/5000 dilution (Purified)

    Lane 1 : 22Rv1 (Human prostate carcinoma epithelial cell) whole cell lysates prepared in 1%SDS Hot lysis method
    Lane 2 : 22Rv1 (Human prostate carcinoma epithelial cell) whole cell lysates prepared in RIPA lysis method

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 99 kDa
    Observed band size: 110 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    This antibody works better in 1%SDS Hot Lysates in WB. For Lysate preparation protocol, please refer to the protocol here (downloadable copy).

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

  • Western blot - Anti-Androgen Receptor antibody [EP670Y] (ab52615)
    Western blot - Anti-Androgen Receptor antibody [EP670Y] (ab52615)
    Anti-Androgen Receptor antibody [EP670Y] (ab52615) at 1/10000 dilution (unpurified) + LnCaP cell lysate at 10 µg

    Secondary
    HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

    Predicted band size: 99 kDa
    Observed band size: 99 kDa

  • Western blot - Anti-Androgen Receptor antibody [EP670Y] (ab52615)
    Western blot - Anti-Androgen Receptor antibody [EP670Y] (ab52615)
    All lanes : Anti-Androgen Receptor antibody [EP670Y] (ab52615) at 1/2000 dilution (purified)

    Lane 1 : Mouse testis tissue lysate
    Lane 2 : Rat testis tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 99 kDa
    Observed band size: 110 kDa why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

  • Anti-Androgen Receptor antibody [EP670Y] (ab52615)
    Anti-Androgen Receptor antibody [EP670Y] (ab52615)

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

データシートおよび資料

  • SDS download

  • Datasheet download

    Download

参考文献 (29)

ab52615 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab52615 は 29 報の論文で使用されています。

  • Zhang Y  et al. Moderate Aerobic Exercise Regulates Follicular Dysfunction by Initiating Brain-Derived Neurotrophic Factor (BDNF)-Mediated Anti-Apoptotic Signaling Pathways in Polycystic Ovary Syndrome. J Clin Med 11:N/A (2022). PubMed: 36233452
  • Wasmuth EV  et al. Allosteric interactions prime androgen receptor dimerization and activation. Mol Cell 82:2021-2031.e5 (2022). PubMed: 35447082
  • Sowalsky AG  et al. Assessment of Androgen Receptor Splice Variant-7 as a Biomarker of Clinical Response in Castration-Sensitive Prostate Cancer. Clin Cancer Res 28:3509-3525 (2022). PubMed: 35695870
  • Kim S  et al. AR-V7 exhibits non-canonical mechanisms of nuclear import and chromatin engagement in castrate-resistant prostate cancer. Elife 11:N/A (2022). PubMed: 35848798
  • Cheaito K  et al. Establishment and characterization of prostate organoids from treatment-naïve patients with prostate cancer. Oncol Lett 23:6 (2022). PubMed: 34820005
View all Publications for this product

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

1-10 of 10 Abreviews or Q&A

ELISA abreview for Anti-Androgen Receptor antibody [EP670Y]

Average
Abreviews
Abreviews
abreview image
Application
ELISA
Sample
Human Cell (HEK293T cells)
Specification
HEK293T cells
Blocking step
Superblock (Thermo Fisher) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Type
Sandwich (Detection)
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Sep 29 2021

Western blot abreview for Anti-Androgen Receptor antibody [EP670Y]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (MEFs)
Gel Running Conditions
Reduced Denaturing
Loading amount
25 µg
Specification
MEFs
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Mar 05 2021

Western blot abreview for Anti-Androgen Receptor antibody [EP670Y]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (LNCaP cells)
Gel Running Conditions
Reduced Denaturing (4-15%)
Loading amount
25 µg
Specification
LNCaP cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Mar 05 2021

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Androgen Receptor [EP670Y] antibody

Good
Abreviews
Abreviews
This product is known to not work in this application or species.
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C
Antigen retrieval step
None
Sample
Hamster Tissue sections (neuronal (amygdala)cells)
Specification
neuronal (amygdala)cells
Permeabilization
No
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Mar 26 2014

Immunocytochemistry abreview for Anti-Androgen Receptor antibody [EP670Y]

Average
Abreviews
Abreviews
Application
Immunocytochemistry
Sample
Human Cell (Human AR receptor construct expressed in live dros)
Specification
Human AR receptor construct expressed in live dros
Fixative
Paraformaldehyde
Permeabilization
Yes - PBS-Triton(0.3%)
Blocking step
PBST/10%Goat serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Apr 26 2012

Question

Thank you very much for your reply.



I would like to use the antibodies against Androgen receptor for detection of androgen receptor in mouse brain floating tissue sections. Is you warranty covering also this application? On your website you mostly show images for prostate, which has much higher expression levels than the brain, so therefore, I would like to check whether your warranty also cover brain tissue for that specific antibody.



Interestingly, though, some our colleagues did use antibody 1852-1 successfully (at least they showed photomicrographs) for detecting AR in mouse brain tissue.



Best regards,

Read More

Abcam community

Verified customer

Asked on Nov 05 2012

Answer

Thank you very much for your interest our products.


To our knowledge, ab133273 not been tested in IHC-FrFl (Immunohistochemistry - Free Floating).

However by participating in our AbTrial program you can now use our products in an untested application or species without financial risk.

Simply follow these easy steps below to apply for our AbTrial Program:

1. Reply to this email, letting us know you are interested in testing this product.

2. Our scientists will email you an inactive personal discount code for the value of the product.

3. Purchase and test the product at the regular price.

4. Submit your results, including your discount code in the additional notes section of your Abreview.

5. Once the Abreview is submitted, the discount code will become active.

6. Apply your discount code on your next order to receive that value off.

Please let me know if you have any questions about this offer and I would be happy to help you further.

I am unable to offer this for ab52615 since it has been tested un-suitable for ICC/IF , IHC-P or IP by our lab.

The Terms and Conditions of this offer can be found at: www.abcam.com/abtrial.

Read More

Abcam Scientific Support

Answered on Nov 05 2012

Question

Dear sir/madam,
I am planning to purchase 1852-1 antibodies against androgen receptor through Abcam Europe. I would like to use this antibody for immunohistochemistry on floating brain sections (murine), as one my colleague has already done. However, since on your website you claim that antibody 3165-1 is suitable for immunohistochemistry while 1852-1 is not (although my colleague has used it and it worked) I would like to ask you if it would be possible to get, together with purchased 1852-1 antibody, also a small sample of 3165-1 antibody so I can compare the two which one might be better for our particular application?
Thank you very much in advance!
Yours sincerely,

Read More

Abcam community

Verified customer

Asked on Nov 01 2012

Answer

Thank you for your inquiry.

I can confirm that in our hands ab52615 (1852-1) is not working in IHC, ICC and IP. Therefore this antibody is not covered by our guarantee since we are not convinced that ab52615 is suitable for these applications.

I can strongly recommend to use ab133273 (3165-1) for your IHC-P experiments. This antibody has been tested to work and we therefore can guarantee this for you.

https://www.abcam.com/Androgen-Receptor-antibody-EPR15352-ab133273.html (or use the following: https://www.abcam.com/Androgen-Receptor-antibody-EPR15352-ab133273.html).

https://www.abcam.com/abpromise

I am sorry to confirm that because we carry over 90,000 products, it isn't feasible for us to keep small sample sizes of our products.

I hope this information is helpful. Please do not hesitate to contact us again with any further questions.

Read More

Abcam Scientific Support

Answered on Nov 01 2012

Question

Phone call enquiring if ab52615 had been used in ICC, or if a sample portion could be sent.

Read More

Abcam community

Verified customer

Asked on Mar 22 2012

Answer

Thank you for contacting us and your interest in our products.

As discussed over the phone, we do not provide tester samples of our antibodies simply due to the logistics that would be involved in this. With over 70,000 products it simply isn't feasible. We do however have a testing discount scheme. As discussed over the phone, this would involve you purchasing ab52615, testing it with your samples with ICC, then sharing the results with us through an Abreview. This should only take 5-10 minutes and provided invaluable information for our customers. As a reward for sharing this information with us (whether the results are positive or negative) you would then be entitled to a free primary antibody of your choice from our catalogue. The only limitation is that the whole process needs to be completed within 4 months. More information on this scheme can be found from the following link:

https://www.abcam.com/collaboratordiscount

I have generated a discount code for you:

Discount code: xxxxx


The expiration date of this code is the 24th of July 2012.

Once you have used ab52615 in your experiments please put this code in the "additional comments" box of the Abreview form. More information on how to submit an Abreview can be found from the following link:

https://www.abcam.com/abreview

Once the review has been published, the code is active and can be used against your next purchase of a primaryantibody. When placing your order for the "free"antibody please quote the discount code.

I hope this information has been of help. If you require any further information please do not hesitate to contact us again.

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Abcam Scientific Support

Answered on Mar 22 2012

Question

Dear Tech support   One of our customers has ordered ab9474. The customer wishes to know what are the chances of the antibody working With Mouse samples? Also, is there a positive control available for this antibody? Thanks in advance for your kind help. Regards,

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Abcam community

Verified customer

Asked on Oct 10 2011

Answer

Thank you for your interest. It is very likely that ab9474 works in mouse but we have no data yet. According to Blast search, the homology between human and mouse AR is very high. http://www.uniprot.org/blast/uniprot/2011101050RXMELAA8 Furthermore, we have some alternative antibodies against AR in our catalogue which proved to recognize mouse AR: ab2742, ab74272, ab3509, ab52615 Normal brain, epididymis, prostate, seminal vesicle; or skin and prostate cancer can be used as good positive control. I hope this helps and if I can assist further, please do not hesitate to contact me.

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Abcam Scientific Support

Answered on Oct 10 2011

Question

I am using your anti-AR antibody, Anti-Androgen Receptor antibody [EP670Y] (ab52615).

According to your website, this antibody’s immunogen is from the residues on the C-terminus of human Androgen Receptor.

I would like to know the peptide mapping of this antibody exactly.

I would be most grateful if you could inform me this information.

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Abcam community

Verified customer

Asked on May 02 2012

Answer

Thank you for contacting Abcam.
The peptide maps between aa ********** on the C-terminus of human Androgen receptor (UniProt: P10275).
Please let me know if there is anything else I can help with you.

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Abcam Scientific Support

Answered on May 02 2012

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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