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AB9474

Anti-Androgen Receptor 抗体 [AR 441]

Anti-Androgen Receptor antibody [AR 441]

4

(12 Reviews)

|

(50 Publications)

Anti-Androgen Receptor antibody [AR 441] (ab9474) is a mouse monoclonal antibody detecting Androgen Receptor in Flow Cytometry, IHC-P. Suitable for Human.

- Over 50 publications
- Trusted since 2002

別名を表示する

DHTR, NR3C4, AR, Androgen receptor, Dihydrotestosterone receptor, Nuclear receptor subfamily 3 group C member 4

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [AR 441] (AB9474)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [AR 441] (AB9474)

ab9474 staining human prostate by IHC-P.

Flow Cytometry - Anti-Androgen Receptor antibody [AR 441] (AB9474)
  • Flow Cyt

Unknown

Flow Cytometry - Anti-Androgen Receptor antibody [AR 441] (AB9474)

Overlay histogram showing PC3 cells stained with ab9474 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab9474, 1/10 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a decreased signal in PC3 cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

Flow Cytometry - Anti-Androgen Receptor antibody [AR 441] (AB9474)
  • Flow Cyt

Unknown

Flow Cytometry - Anti-Androgen Receptor antibody [AR 441] (AB9474)

Overlay histogram showing MCF-7 cells stained with ab9474 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab9474, 1/10 dilution) for 30 min at 22°C. The secondary antibody used was DyLight ® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF-7 cells fixed with methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

AR 441

アイソタイプ

IgG1

軽鎖のタイプ

unknown

キャリアフリー

No

交差種

Human

アプリケーション

IHC-P, Flow Cyt

applications

免疫原

Synthetic Peptide within Human AR aa 300-350. The exact immunogen used to generate this antibody is proprietary information.

P10275

特異性

This antibody is specific to a protein of 110 kD, identified as androgen receptor. This antibody reacts with full length androgen receptor and also with the newly described A form of the receptor. This antibody does not cross react with estrogen, progesterone or glucocorticoid receptors.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/25 - 1/50", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p><a href='/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" }, "Macaque monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" } } }
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製品の詳細

What is this antibody validated in?
Anti-Androgen Receptor antibody [AR 441] (ab9474) is a mouse monoclonal antibody and is validated for use in Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P) in Human samples.

Trusted by the scientific community
Anti-Androgen Receptor [AR 441] (ab9474) was first used in a scientific publication in 2002 and has been cited over 50 times in peer-reviewed journals.

Reviewed by scientists
Anti-Androgen Receptor [AR 441] (ab9474) has over 10 independent reviews from customers.
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein G
精製に関する特記事項
purified immunoglobulin fraction.
バッファー組成
pH: 7.3 - 7.5 Preservative: 0.09% Sodium azide Constituents: PBS
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The androgen receptor (AR) also known as NR3C4 functions mechanically as a type of nuclear receptor that is activated by binding androgens including testosterone and dihydrotestosterone. The AR has a molecular weight of approximately 110 kDa. It is expressed in various tissues notably in the prostate skeletal muscle and certain areas of the brain. Within cells AR is usually located in the cytoplasm when unbound and translocates to the nucleus upon activation by its ligands.
Biological function summary

AR interacts with androgen hormones to regulate gene expression. It forms a complex with coactivators and corepressors to modulate transcription of target genes involved in male sexual development and reproductive function. AR plays an important role in the development and maintenance of male characteristics and reproductive activity. Its expression impacts cellular processes such as proliferation differentiation and apoptosis.

Pathways

AR is intricately involved in the androgen signaling pathway which affects the regulation of genes related to male sexual characteristics. It also plays a part in the PI3K/Akt pathway influencing cell growth and survival. The AR pathway interacts with proteins such as estrogen receptors to mediate cross-talk between hormonal pathways highlighting its broad biological impacts.

AR has a significant connection to prostate cancer where it drives tumor growth and progression. Mutations and overexpression of the androgen receptor are often observed in prostate cancer cells. Another condition linked to AR is androgen insensitivity syndrome where mutations in the AR gene result in partial or complete inability of cells to respond to androgenic hormones. In these conditions AR antagonists like bicalutamide are often used as treatment inhibiting the receptor's activity to manage disease progression.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Steroid hormone receptors are ligand-activated transcription factors that regulate eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues (PubMed : 19022849). Transcription factor activity is modulated by bound coactivator and corepressor proteins like ZBTB7A that recruits NCOR1 and NCOR2 to the androgen response elements/ARE on target genes, negatively regulating androgen receptor signaling and androgen-induced cell proliferation (PubMed : 20812024). Transcription activation is also down-regulated by NR0B2. Activated, but not phosphorylated, by HIPK3 and ZIPK/DAPK3.. Isoform 3. Lacks the C-terminal ligand-binding domain and may therefore constitutively activate the transcription of a specific set of genes independently of steroid hormones.. Isoform 4. Lacks the C-terminal ligand-binding domain and may therefore constitutively activate the transcription of a specific set of genes independently of steroid hormones.
See full target information AR
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文献 (50)

Recent publications for all applications. Explore the full list and refine your search

Clinical case reports 11:e7310 PubMed37215961

2023

A rare case of a toddler with unilateral cryptorchidism-related cranial suspensory ligament remnant.

Applications

Unspecified application

Species

Unspecified reactive species

Hideaki Nakajima,Kazuto Suda,Atsushi Arakawa,Toshihiro Yanai

Scientific reports 13:4683 PubMed36949059

2023

CACNA1D overexpression and voltage-gated calcium channels in prostate cancer during androgen deprivation.

Applications

Unspecified application

Species

Unspecified reactive species

Niamh McKerr,Adone Mohd-Sarip,Hannah Dorrian,Conor Breen,Jacqueline A James,Stephen McQuaid,Ian G Mills,Karen D McCloskey

Cell death & disease 13:927 PubMed36335093

2022

Androgen deprivation restores ARHGEF2 to promote neuroendocrine differentiation of prostate cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xuanrong Chen,Yi Shao,Wanqing Wei,Shimiao Zhu,Yang Li,Yutong Chen,Hanling Li,Hao Tian,Guijiang Sun,Yuanjie Niu,Zhiqun Shang

The Biochemical journal 479:1519-1531 PubMed35781489

2022

Identification of ELK1 interacting peptide segments in the androgen receptor.

Applications

Unspecified application

Species

Unspecified reactive species

Claire Soave,Charles Ducker,Seongho Kim,Thomas Strahl,Rayna Rosati,Yanfang Huang,Peter E Shaw,Manohar Ratnam

Molecular cancer research : MCR 20:841-853 PubMed35302608

2022

Attenuating Adaptive VEGF-A and IL8 Signaling Restores Durable Tumor Control in AR Antagonist-Treated Prostate Cancers.

Applications

Unspecified application

Species

Unspecified reactive species

Pamela J Maxwell,Melanie McKechnie,Christopher W Armstrong,Judith M Manley,Chee Wee Ong,Jenny Worthington,Ian G Mills,Daniel B Longley,James P Quigley,Amina Zoubeidi,Johann S de Bono,Elena Deryugina,Melissa J LaBonte,David J J Waugh

The Journal of clinical endocrinology and metabolism 107:512-524 PubMed34546342

2021

The Cortisol Response of Male and Female Choroidal Endothelial Cells: Implications for Central Serous Chorioretinopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Joost Brinks,Elon H C van Dijk,Szymon M Kiełbasa,Hailiang Mei,Isa van der Veen,Hendrika A B Peters,Hetty C M Sips,Robbert G E Notenboom,Paul H A Quax,Camiel J F Boon,Onno C Meijer

British journal of cancer 125:1377-1387 PubMed34471256

2021

Apalutamide radio-sensitisation of prostate cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Christos Kakouratos,Dimitra Kalamida,Ioannis Lamprou,Erasmia Xanthopoulou,Christos Nanos,Alexandra Giatromanolaki,Michael I Koukourakis

Journal of cell science 134: PubMed34318896

2021

DNA binding alters ARv7 dimer interactions.

Applications

Unspecified application

Species

Unspecified reactive species

Fatma Özgün,Zeynep Kaya,Tunç Morova,Bart Geverts,Tsion E Abraham,Adriaan B Houtsmuller,Martin E van Royen,Nathan A Lack

Cancer science 112:3871-3883 PubMed34050700

2021

Functional analysis of LAT3 in prostate cancer: Its downstream target and relationship with androgen receptor.

Applications

Unspecified application

Species

Unspecified reactive species

Junryo Rii,Shinichi Sakamoto,Masahiro Sugiura,Manato Kanesaka,Ayumu Fujimoto,Yasutaka Yamada,Maihulan Maimaiti,Keisuke Ando,Ken Wakai,Minhui Xu,Yusuke Imamura,Norihisa Shindo,Toru Hirota,Atsushi Kaneda,Yoshikatsu Kanai,Yuzuru Ikehara,Naohiko Anzai,Tomohiko Ichikawa

Reproduction (Cambridge, England) 161:593-602 PubMed33784244

2021

Testosterone regulation on quiescin sulfhydryl oxidase 2 synthesis in the epididymis.

Applications

Unspecified application

Species

Unspecified reactive species

Tse-En Wang,Shiori Minabe,Fuko Matsuda,Sheng-Hsiang Li,Hiroko Tsukamura,Kei-Ichiro Maeda,Lee Smith,Laura O'Hara,Bart M Gadella,Pei-Shiue Tsai
View all publications
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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。
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